Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex‐sorted bull sperm by inhibiting the oxidative stress

Zhang, Peipei; Wang, Jingjing; Li, Chong-Yang; Huang, Hao; Wang, Haoyu; Du, Weihua; Pang, Yuxin; Zhao, Shanjiang; Zou, Huiying; Tang, Hao; Yang, Shu-Ping; Ruan, Weimin; Huang, Jinming; Jiao, Peng-Cheng; Zhu, Huabin; Zhao, Xiao‐Fan

doi:10.1111/rda.13748

Cited by 7 publications

(7 citation statements)

References 70 publications

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“…Bovine embryos derived from sex-sorted spermatozoa had an increased incidence of arrest at the 4-cell stage, and reduced survival and blastocyst rates [208]. Supplementation of vitamin C or lycopene in washing and fertilization medium improves the fertilization, cleavage, and blastocyst rates of oocytes inseminated with sex-sorted bull sperm [209]. Apoptosis (Annexin V) ↓ (protection) ∆Ψm ↑ (VC: extent bull specific) Effects of endogenous, experimentally induced (Ind.…”

Section: Effects Of Sperm Os On Embryo Developmentmentioning

confidence: 99%

Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

et al. 2021

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Reactive oxygen species (ROS) generated at low levels during mitochondrial respiration have key roles in several signaling pathways. Oxidative stress (OS) arises when the generation of ROS exceeds the cell’s antioxidant scavenging ability and leads to cell damage. Physiological ROS production in spermatozoa regulates essential functional characteristics such as motility, capacitation, acrosome reaction, hyperactivation, and sperm-oocyte fusion. OS can have detrimental effects on sperm function through lipid peroxidation, protein damage, and DNA strand breakage, which can eventually affect the fertility of an individual. Substantial evidence in the literature indicates that spermatozoa experiencing OS during in vitro manipulation procedures in human- and animal-assisted reproduction are increasingly associated with iatrogenic ROS production and eventual impairment of sperm function. Although a direct association between sperm OS and human assisted reproductive techniques (ART) outcomes after in vitro fertilization (IVF) and/or intracytoplasmic sperm injection (ICSI) is still a matter of debate, studies in animal models provide enough evidence on the adverse effects of sperm OS in vitro and defective fertilization and embryo development. This review summarized the literature on sperm OS in vitro, its effects on functional ability and embryo development, and the approaches that have been proposed to reduce iatrogenic sperm damage and altered embryonic development.

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Section: Effects Of Sperm Os On Embryo Developmentmentioning

confidence: 99%

Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

et al. 2021

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“…4. In addition, freezing-thawing induces sperms to produce ROS and lowers the activity of antioxidants in bull (Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…As a result, a series of inflammatory events occur, resulting in membrane integrity loss, increased permeability, structural DNA damage and apoptosis (Alahmar, 2019). In addition, freezing–thawing induces sperms to produce ROS and lowers the activity of antioxidants in bull (Zhang et al, 2020). …”

Section: Introductionmentioning

confidence: 99%

Ameliorative effect of chitosan nanoparticles in capacitation media on post‐thawing in vitro fertilizing ability of bovine spermatozoa

Mahmoud,

Abd‐Allah,

Abdel‐Halim

et al. 2023

Reprod Domestic Animals

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This study aimed to investigate the effect of supplementation of chitosan nanoparticles (CSNPs) on the capacitation of bovine spermatozoa during the in vitro fertilization process. Hyperactivated motility (HAM) and acrosome reaction (AR) of sperm cells as well as in vitro fertilization and cleavage rates are the main parameters used to estimate the effect of CSNPs on bovine spermatozoa's fertilizing ability. In this study, three different concentrations of CSNPs (10, 20 and 100 μg/mL) were prepared and characterized. Motile spermatozoa were separated from frozen–thawed semen by a swim‐up technique and capacitated in Sperm‐TALP medium supplemented with heparin only without CSNPs treatment (positive control), heparin + 10 μg/mL CSNPs, heparin + 20 μg/mL CSNPs, heparin + 100 μg/mL CSNPs and the last one served as a negative control tube which supplemented with 10 μg/mL CSNPs without adding heparin. Sperm cells were incubated for 90 min at 39°C in a 5% CO2 incubator and evaluated every 30 min at intervals. Cumulus oophorus complex (COCs) were matured in a 5% CO2 incubator at 39°C and inseminated in vitro with frozen–thawed bull sperm of the above concentrations. The inseminated oocytes were incubated at 39°C in a 5% CO2 incubator for 24 h and then examined for evidence of fertilization. The results of this investigation showed that HAM and AR were best affected by CSNPs at a concentration of 20 μg/mL during an incubation time of 60 min. As time went on, the overall proportion of spermatozoa with progressive motility (PM) decreased across all groups, and a substantially lower value was found at the dose mentioned above. Additionally, the impact of sperm treated with CSNPs on fertilization rate was assessed. The outcomes demonstrated that in comparison to the other concentrations (10 and 100 μg/mL), the positive control and the negative control, the proportion of fertilized oocytes was significantly higher in the CSNPs concentration (20 μg/mL). In conclusion, it could be inferred from this investigation that CSNPs support sperm functions during IVF and can be used for biomedical interventions in bovine spermatozoa. Additionally, a high IVF rate was achieved by using sperm treated with CSNPs as CSNPs enhance sperm capacitation and acrosome reaction.

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“…Sexing control is an important technique in livestock breeding that facilitates the production of offspring of a specific sex, according to the farmers’ preference, through specific interventions [ 1 ]. In livestock production, the demand for specific animal products often results in the need for biased sex control of animal offspring [ 2 ]. For example, the production of goat milk requires female dairy goats.…”

Section: Introductionmentioning

confidence: 99%

TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos

Ren

Hao

Ren

et al. 2021

J Animal Sci Biotechnol

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Background Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion. To derive greater economic benefit, farmers require more female offspring (does); however, the buck-to-doe offspring sex ratio is approximately 50%. At present, artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock offspring. However, flow cytometry has not been successfully utilised for the separation of X/Y sperm aimed at sexing control in dairy goats. Results In this study, a novel, simple goat sperm sexing technology that activates the toll-like receptor 7/8 (TLR7/8), thereby inhibiting X-sperm motility, was investigated. Our results showed that the TLR7/8 coding goat X-chromosome was expressed in approximately 50% of round spermatids in the testis and sperm, as measured from cross-sections of the epididymis and ejaculate, respectively. Importantly, TLR7/8 was located at the tail of the X-sperm. Upon TLR7/8 activation, phosphorylated forms of glycogen synthase kinase α/β (GSK3 α/β) and nuclear factor kappa-B (NF-κB) were detected in the X-sperm, causing reduced mitochondrial activity, ATP levels, and sperm motility. High-motility Y-sperm segregated to the upper layer and the low-motility X-sperm, to the lower layer. Following in vitro fertilisation using the TLR7/8-activated sperm from the lower layer, 80.52 ± 6.75% of the embryos were XX females. The TLR7/8-activated sperm were subsequently used for in vivo embryo production via the superovulatory response; nine embryos were collected from the uterus of two does that conceived. Eight of these were XX embryos, and one was an XY embryo. Conclusions Our study reveals a novel TLR7/8 signalling mechanism that affects X-sperm motility via the GSK3 α/β-hexokinase pathway; this technique could be used to facilitate the efficient production of sexed dairy goat embryos.

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Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex‐sorted bull sperm by inhibiting the oxidative stress

Cited by 7 publications

References 70 publications

Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

Ameliorative effect of chitosan nanoparticles in capacitation media on post‐thawing in vitro fertilizing ability of bovine spermatozoa

TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos

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