Protective Effect of Water-Soluble C60 Fullerene Nanoparticles on the Ischemia-Reperfusion Injury of the Muscle Soleus in Rats

Abstract

The biomechanical parameters of muscle soleus contraction in rats and their blood biochemical indicators after the intramuscular administration of water-soluble C60 fullerene at doses of 0.5, 1, and 2 mg/kg 1 h before the onset of muscle ischemia were investigated. In particular, changes in the contraction force of the ischemic muscle soleus, the integrated power of the muscle, the time to achieve the maximum force response, the dynamics of fatigue processes, and the parameters of the transition from dentate to smooth tetanus, levels of creatinine, creatine kinase, lactate and lactate dehydrogenase, and parameters of prooxidant–antioxidant balance (thiobarbituric acid reactive substances, hydrogen peroxide, and reduced glutathione and catalase) were analyzed. The positive therapeutic changes in the studied biomechanical and biochemical markers were revealed, which indicate the possibility of using water-soluble C60 fullerenes as effective prophylactic nanoagents to reduce the severity of pathological conditions of the muscular system caused by ischemic damage to skeletal muscles.

Molecule-to-Material-to-Bio Nanoarchitectonics with Biomedical Fullerene Nanoparticles

“…C 60 fullerenes can efficiently capture free radicals with their abundant conjugated double bonds and low-energy LUMO orbitals. Ritter and coworkers investigated intramuscularly administered C 60 fullerene aqueous solution for therapeutic and preventive value as an antioxidant [ 173 ]. The study found that C 60 fullerene aqueous solution at a dose of 1 mg/kg reduces severity of ischemic damage in rat muscle soleus by 60–75%.…”

Comparative Analysis of the Effect of Antioxidants C60-Fullerene and N-Acetylcysteine on the Correction of the Retention Time of the Maximum Level of Contraction of Skeletal Muscles of Rats During the Development of Muscle Fatigue

“…Молекулі С 60 притаманна висока відновлювальна здатність; вона може приєднувати до шістьох електронів одночасно. Завдяки цій властивості С 60фуллерени та їхні похідні діють у біологічних системах як ефективні вловлювачі вільних радикалів, зокрема активних форм кисню (ÀÔК), гіперпродукція яких веде до виникнення багатьох патологій [16][17][18], включаючи ішемічні ушкодження м'язів [19,20]. Жодних токсичних ефектів не було зафіксовано за досліджень дії водорозчинних С 60 -фуллеренів у системах in vitro та in vivo [21,22].…”

Effect of C60 Fullerene on Recovery of Muscle Soleus in Rats after Atrophy Induced by Achillotenotomy

“…In our previous studies, it was shown that administration of water-soluble C 60 fullerenes after initiation of ischemic damage leads to significant positive therapeutic effects [ 23 ]. A positive trend has been shown when using them for muscle injury [ 24 ], muscle dysfunction associated with pesticide poisoning [ 25 ], as well as the development of fatigue processes [ 26 ]. All these data stimulated us to test water-soluble C 60 fullerenes as potential therapeutic agents that reduce pathological effects in the muscular system of rats during the development of AT-associated dystrophy.…”