Protective effect of XY99-5038 on hydrogen peroxide induced cell death in cultured retinal neurons

Yan, Xin; Fong, Yuhay T.; Wolf, Gerald; Wolf, David E.; Cao, Wei

doi:10.1016/s0024-3205(01)01122-5

Cited by 23 publications

(13 citation statements)

References 21 publications

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“…Similar to our findings reported here, a cell culture medium treated by YXQ-EQ induced apoptosis in non-small cell lung cancer cells and an YXQ-EQ treated natural plant extract protected neurons against oxidative stress induced apoptosis [20,29]. More importantly, long-term clinical observations have seen anticancer effects of XY-S [9,11,12].…”

Section: Discussionsupporting

confidence: 84%

“…Previous studies have shown that YXQ-EQ treated PBS (named as YXQ-EQ water or YXQ-EQW), YXQ-EQ treated cell culture medium, and YXQ-EQ treated natural nontoxic plant extracts (XY99-5038 and XY-S) possess some biological effects of YXQ-EQ, including anticancer, antiviral and antioxidative activity [9,11,12,20,29,30]. Therefore, we examined whether YXQ-EQW and XY-S could inhibit the growth of HT-29 cells.…”

Section: Resultsmentioning

confidence: 99%

“…No cytotoxicity to normal cells was observed in these studies with YXQ-EQ. In fact, in vitro studies have found that YXQ-EQ was capable of protecting neurons from oxidative stress induced apoptosis and significantly prolonged survival of neurons [20,21]. …”

Section: Introductionmentioning

confidence: 99%

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External Qi of Yan Xin Qigong Inhibits Activation of Akt, Erk/12 and NF-ĸB and Induces Cell Cycle Arrest and Apoptosis in Colorectal Cancer Cells

Yan¹,

Shen²,

Jiang

et al. 2013

Cell Physiol Biochem

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Background/Aims: Colorectal cancer (CRC) is the second leading cause of cancer death in the Western countries. Novel approaches of treatment are needed for CRC. The purpose of the present study was to investigate cytotoxic effect of external Qi of Yan Xin Qigong (YXQ-EQ) on human colorectal cancer cells. Methods: The effect of YXQ-EQ on viability, cell cycle progression and apoptosis in colorectal cancer HT-29 cells was investigated. Phosphorylation of Akt and Erk1/2, activation of NF-ĸB and the expression of proteins involved in regulation of cell cycle and apoptosis were examined by Western blot analysis. Results: YXQ-EQ markedly decreased viability and blocked colony formation of HT-29 cells. YXQ-EQ downregulated cyclin D1 expression and increased accumulation of cyclin-dependent kinase inhibitors p21^Cip1 and p27^Kip1, resulting in G1 cell cycle arrest. YXQ-EQ induced apoptosis in HT-29 cells in association with decreased expression of antiapoptotic proteins Bcl-xL, XIAP, survivin and Mcl-1 and elevated expression of proapoptotic protein Bax. YXQ-EQ significantly repressed phosphorylation of Akt and Erk1/2 and NF-ĸB activation in HT-29 cells, suggesting that YXQ-EQ may exert cytotoxic effect through regulating signaling pathways critical for cell proliferation and survival. Furthermore, YXQ-EQ treated PBS and an YXQ-EQ treated plant extract induced apoptosis in HT-29 cells. Conclusion: These findings show that YXQ-EQ has potent cytotoxic effect on HT-29 cells and suggest that YXQ-EQ could be potentially used for colorectal cancer treatment either directly or indirectly via carriers.

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Section: Discussionsupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

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External Qi of Yan Xin Qigong Inhibits Activation of Akt, Erk/12 and NF-ĸB and Induces Cell Cycle Arrest and Apoptosis in Colorectal Cancer Cells

Yan¹,

Shen²,

Jiang

et al. 2013

Cell Physiol Biochem

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“…Susceptibility of the cells of the central nervous system to the action of H 2 O 2 has also been reported. Thus H 2 O 2 caused cytotoxicity by inducing apoptosis in cultured retinal neurons, cortical neuron, cerebellar granule neuron and oligodendroglia [30,17,31,32]. H 2 O 2 has been reported to directly stimulate endonuclease activity in renal tubular epithelial cells, leading to DNA fragmentation and cell death via apoptosis [33].…”

Section: Discussionmentioning

confidence: 99%

Comparative effects of α-tocopherol and γ-tocotrienol against hydrogen peroxide induced apoptosis on primary-cultured astrocytes

Mazlan¹,

Then²,

Top³

et al. 2006

Journal of the Neurological Sciences

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“…Studies over the past three decades have demonstrated influence of YXQ-EQ on both the structural and functional properties of biomolecules [17][18][19][20][21][22]. Furthermore, YXQ-EQ has been shown to induce apoptosis in cancer cells while protect neurons from oxidative stress induced cell death via modulating signaling pathways and gene expression [6][7][8][9][10][11]23]. These studies provide some molecular and cellular insights into the observed clinical benefits of YXQ-EQ.…”

Section: Introductionmentioning

confidence: 99%

YXQ-EQ Induces Apoptosis and Inhibits Signaling Pathways Important for Metastasis in Non-Small Cell Lung Carcinoma Cells

Yan¹,

Shen²,

Jiang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Lung cancer is one of the most prevalent malignancies in the world. The 5-year survival rate for non-small cell lung cancer (NSCLC) patients is only approximately 15%, with metastasis as the primary cause of death. This study was aimed to investigate cytotoxic effect of external qi of Yan Xin Qigong (YXQ-EQ) toward human lung adenocarcinoma A549 cells as well as its effect on signaling pathways promoting migration, invasion and epithelial-to-mesenchymal transition (EMT) in A549 cells. Methods: Cytotoxic effect of YXQ-EQ was evaluated using MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] and cologenic assays. Apoptosis of treated cells was determined by Annexin V/propidium iodide staining and flow cytometry analysis, while cell migration and invasion were determined using transwell assays and EMT was assessed by morphological changes in cells. Protein expression and phosphorylation were examined by immunoblot analyses. Results: YXQ-EQ induced apoptosis in A549 cells, resulting in a pronounced reduction in viability and clonogenic formation. This was associated with inhibition of phosphorylation of AKT and ERK1/2 and reduced expression of anti-apoptotic proteins BCL-xL, XIAP and survivin. Furthermore, YXQ-EQ inhibited EGF/EGFR signaling and EGF mediated migration and invasion of A549 cells. While TGF-β1 induced phosphorylation of SMAD2/3 and EMT in A549 cells, YXQ-EQ suppressed TGF-β/SMAD signaling and induced cell death in these cells in the presence of TGF-β1. Conclusion: Our findings suggest that YXQ-EQ could exert anti-lung cancer effects via inhibiting signaling pathways that are important for NSCLC cell survival and NSCLC metastasis.

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Protective effect of XY99-5038 on hydrogen peroxide induced cell death in cultured retinal neurons

Cited by 23 publications

References 21 publications

External Qi of Yan Xin Qigong Inhibits Activation of Akt, Erk/12 and NF-ĸB and Induces Cell Cycle Arrest and Apoptosis in Colorectal Cancer Cells

External Qi of Yan Xin Qigong Inhibits Activation of Akt, Erk/12 and NF-ĸB and Induces Cell Cycle Arrest and Apoptosis in Colorectal Cancer Cells

Comparative effects of α-tocopherol and γ-tocotrienol against hydrogen peroxide induced apoptosis on primary-cultured astrocytes

YXQ-EQ Induces Apoptosis and Inhibits Signaling Pathways Important for Metastasis in Non-Small Cell Lung Carcinoma Cells

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