Protective effect ofSpirulina against doxorubicin-induced cardiotoxicity

Khan, Mahmood; Shobha, J. C.; Mohan, Iyyapu Krishna; Naidu, M.U.R.; Challa, Sundaram; Shashi, Singh; Kuppusamy, Periannan; Kutala, Vijay Kumar

doi:10.1002/ptr.1783

Cited by 148 publications

(104 citation statements)

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“…Nascimento & Martins (2005) relataram lesões cardíacas ultramicroscópicas induzidas pela DOX semelhantes às descritas nesta pesquisa. Resultados semelhantes também foram descritos por Khan et al (2005), em camundongos tratados com dose acumulada de 16mg/kg de DOX, por via intraperitoneal. Por outro lado, Dudnakova et al (2003), em análise ultramicroscópica, três semanas após a aplicação de 2,2mg/kg de DOX, observaram perda focal de elementos contráteis, alterações no citoesqueleto e drástica alteração na permeabilidade nuclear nos cardiomiócitos de ratos tratados com DOX, alterações estas não detectadas na presente pesquisa, o que pode estar relacionado à dose e ao tempo decorrido entre a aplicação da DOX e a avaliação morfológica.…”

Section: Discussionunclassified

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Ação do extrato etanólico da casca do pequi (Caryocar brasiliense) na cardiotoxicidade crônica induzida por doxorrubicina em ratos

et al. 2017

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ABSTRACT.-Moura L.R., Orpinelli S.R.T., Sousa J.H., Faleiro M.B.R., Conceição E. Goiânia, Doxorubicin (DOX) is a chemotherapic drug used in the treatment of malignancies, but has the cardiotoxicity as collateral effect. The objective of this study was to evaluate the action of pequi shell etanolic extract (Caryocar brasiliense) (PSEE) through morphological evaluation (macroscopic, microscopic and ultramicroscopic), and to evaluate the expression of metalloproteinases (MMP2 and MMP9) and its tissue inhibitors (TIMP1 and TIMP2) in the myocardium of rats with chronic cardiotoxicity by DOX and treated or not with PSEE. The experiment lasted three months and 30 Wistar rats were divided into six groups of five animals. G1 and G2 received 300mg/kg and 600mg/kg of PSEE, respectively, as pretreatment, by gavage for seven days and continued treatment for 21 days of application of DOX. In G1, G2, G3, G4 and GC, cardiotoxicity was induced with weekly applications of 2mg/kg DOX, intraperitoneally, totaling four applications (8 mg/kg), and in the Sham group (GS) 1ml of saline solution was applied. G3 animals received daily 300mg/ kg of PSEE, and G4, 600mg/kg, by gavage, for 21 days of application of DOX. The GC and GS received 1ml of water daily by gavage also. After the completion of the application, the animals were kept for two months, with three months of experiment. Macroscopic evaluation was performed after 90 days, at which time samples were taken for analysis in electron microscopy, histopathology and immunohistochemistry. At necropsy, ascites was observed in animals that received DOX. There was a low mortality rate (3.33%), being one mouse that developed false road pneumonia. There was no change in weights and measures of the rat hearts. At doses of 300 and 600mg/kg, the PSEE attenuates myocyte vacuolar degeneration. At a dose of 600mg/kg, PSEE reduces amount Anitschkow cells. There was no significant result on the immunostaining of MMP, but considering their inhibitors (TIMP) there was a greater immunostaining of TIMP2 in GC, the group that received only DOX. It was concluded that PSEE is effective in minimizing effects of chronic cardiotoxicity induced by DOX in the myocardium of rats, whereas at doses of 300 and 600mg/ 1 Recebido em 13 de abril de 2016.Aceito para publicação em 12 de setembro de 2016.

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Section: Discussionunclassified

“…Khan et al (2005) relataram que a Spirulina protegeu significativamente camundongos dos efeitos cardiotóxicos da DOX. Xin et al (2011) relataram que o pré-tratamento com polissacarídeo de Lycium barbarum reduziu significativamente o estresse oxidativo induzido pela DOX no tecido cardíaco.…”

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Ação do extrato etanólico da casca do pequi (Caryocar brasiliense) na cardiotoxicidade crônica induzida por doxorrubicina em ratos

et al. 2017

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“…One-third of all indigenous inhabitants of Sub-Saharan Africa carry the gene, because in areas where malaria is common, there is a fitness benefit in carrying only a single sickle-cell gene (sickle cell trait). Those with only one of the two alleles of the sickle-cell disease, while not totally resistant are more tolerant to the infection and thus show less severe symptoms when infected [2][3][4] . Sickle-cell anaemia is the name of a specific form of Sickle-cell disease in which there is homozygosity for the mutation that causes HbS 1 .…”

Section: Introductionmentioning

confidence: 99%

Effects of Athrospira Platensis, Monodora Myristica and Helianthus Annuus on the Rate of Polymerization, Sickle Cell Reversion and Oxygen Affinity of Sickle Cell Hemoglobin

Nwaichi¹,

Monago²,

Amaraegbulem³

2013

J Pharm Sci Innov

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The in vitro effects of the cyanobacteria (Athrospira platensis), spices (Monodora myristica) and the achene (Hellianthus annuus) on the rate of polymerization, sickle cell reversion and oxygen affinity of sickle cell hemoglobin (HbS) was studied spectophotometrically using hemolysates of HbS containing erythrocytes treated with sodium metabisulphite in the presence of the different plant extracts. All fractions of the three plant extracts showed statistically significant decreases (p< 0.05) in the rate of polymerization therefore inhibited the rate of sickling with increasing time. The Benzene Soluble Extract (BESE) fraction of Hellianthus annuus demonstrated the highest antisickling activity (89.15% inhibition of HbSS), while the least was by the BESE fraction of Athrospira platensis (45.16% inhibition of HbSS). The crude aqueous extract (CAE) fraction of Athrospira platensis showed the highest antioxidant effect, while CAE fraction of Monodora myristica gave the least. The water soluble extract (WSE) fraction of Hellianthus annuus expressed maximum rate of sickling reversion. Alcohol extracts of these plants were found to exhibit a more significant antisickling activity relative to the aqueous extracts which is attributable to the presence of some lipophillic amino acids present in the alcoholic fraction of the extracts, and it could also be responsible for the observed high reversion capacity but low antioxidant effect of the lipophillic fraction of the plant extracts.

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“…Antioxidants exist in a variety of non-enzymatic forms, which include Vitamin C, Vitamin E, and Carotenoids. Thus, antioxidants play an important role in the protection of cells against oxidative damage caused by Reactive Oxygen species (ROS) 11 . ROS, such as superoxide-anion, hydroxyl radicals and hydrogen peroxide are generated by general physiological processes and by various exogenous factors that initiat peroxidation of membrane lipids as well as a wide range of other biological molecules through a process that is believed to be implicated in the etiology of several disease conditions including Coronary heart disease, Stroke, Rheumatoid arthritis, Diabetes and Cancer.…”

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confidence: 99%

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2017

IJPSR

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ABSTRACT:Microalgae have been widely used as novel source of bioactive substances. These substances exhibit various biological actions including, antioxidant, antimicrobial, antiviral, antitumoral, antiinflammatory and anti-allergy effects. In the present investigation algal biomass in methanol extracts of chlorophyceae member of three micro algae of Chlorella vulgaris, Desmococcus olivaceous and Chlorococcum humicola was screened. The antioxidant property of the methanolic extracts of these green microalgae was evaluated by measuring the free radical scavenging activity by DPPH assay method. The algal extracts were then evaluated for their suppressive effect on tumor cell growth (MCF7) by using MTT assay and further analysed using GC-MS to determine the profile of specific molecules or compounds. Antioxidant activities of the microalgal methanol extracts were studied by their ability to scavenge DPPH at various concentrations. The DPPH radical scavenging activity was found to be higher in Chlorella vulgaris (RSA 53.96%) at a concentration 20μg compared to standard ascorbic acid (RSA 59.42%). Anticancer property of the methanol extracts of Chlorella vulgaris, Desmococcus olivaceous and Chlorococcum humicola in MCF7 breast cancer cell was studied. In this assay the micro algal Chlorella vulgaris inhibited the growth of tumor cells (MCF7) when compared to Desmococcus olivaceous. GC-MS analysis of methanol extracts of Chlorococcum humicola showed the presence of five compounds which are reported to be involved in antioxidant and anticancer properties. INTRODUCTION:Microalgae produce metabolites in order to survive under varying conditions of salinity, temperature, pressure etc. These metabolites are usually secondary, which could be carbohydrate, or proteins or enzymes or pigments or antibiotics or even toxic compounds.

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Protective effect ofSpirulina against doxorubicin-induced cardiotoxicity

Cited by 148 publications

References 68 publications

Ação do extrato etanólico da casca do pequi (Caryocar brasiliense) na cardiotoxicidade crônica induzida por doxorrubicina em ratos

Ação do extrato etanólico da casca do pequi (Caryocar brasiliense) na cardiotoxicidade crônica induzida por doxorrubicina em ratos

Effects of Athrospira Platensis, Monodora Myristica and Helianthus Annuus on the Rate of Polymerization, Sickle Cell Reversion and Oxygen Affinity of Sickle Cell Hemoglobin

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