Protective effects and active ingredients of Salvia miltiorrhiza Bunge extracts on airway responsiveness, inflammation and remodeling in mice with ovalbumin-induced allergic asthma

Luo, Jun; Zhang, Li; Zhang, Xinyi; Long, Yan; Zou, Feng-Cai; Yan, Chunsong; Zou, Wei

doi:10.1016/j.phymed.2018.09.170

Cited by 40 publications

(20 citation statements)

References 22 publications

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“…Previous studies showed that Salvianolic acid B and sodium tanshinone II A sulfonate could prevent pulmonary fibrosis through anti-inflammatory and anti-fibrotic process [ 21 ]. Ovalbumin-sensitized allergic asthma could be ameliorated with the treatment of Salvia miltiorrhiza water extraction in mice [ 22 ]. Our previous results also indicated that Salvianolic acid C has shown the ability of inhibiting SARS-CoV-2 infection [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation

Wang

et al. 2021

Acta Pharmacol Sin

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can induce acute inflammatory response like acute lung inflammation (ALI) or acute respiratory distress syndrome, leading to severe progression and mortality. Therapeutics for treatment of SARS-CoV-2-triggered respiratory inflammation are urgent to be discovered. Our previous study shows that Salvianolic acid C potently inhibits SARS-CoV-2 infection. In this study, we investigated the antiviral effects of a Salvia miltiorrhiza compound, Danshensu, in vitro and in vivo, including the mechanism of S protein-mediated virus attachment and entry into target cells. In authentic and pseudo-typed virus assays in vitro, Danshensu displayed a potent antiviral activity against SARS-CoV-2 with EC 50 of 0.97 μM, and potently inhibited the entry of SARS-CoV-2 S protein-pseudo-typed virus (SARS-CoV-2 S) into ACE2-overexpressed HEK-293T cells (IC 50 = 0.31 μM) and Vero-E6 cell (IC 50 = 4.97 μM). Mice received SARS-CoV-2 S via trachea to induce ALI, while the VSV-G treated mice served as controls. The mice were administered Danshensu (25, 50, 100 mg/kg, i.v., once) or Danshensu (25, 50, 100 mg·kg -1 ·d -1 , oral administration, for 7 days) before SARS-CoV-2 S infection. We showed that SARS-CoV-2 S infection induced severe inflammatory cell infiltration, severely damaged lung tissue structure, highly expressed levels of inflammatory cytokines, and activated TLR4 and hyperphosphorylation of the NF-κB p65; the high expression of angiotensinogen (AGT) and low expression of ACE2 at the mRNA level in the lung tissue were also observed. Both oral and intravenous pretreatment with Danshensu dose-dependently alleviated the pathological alterations in mice infected with SARS-CoV-2 S. This study not only establishes a mouse model of pseudo-typed SARS-CoV-2 (SARS-CoV-2 S) induced ALI, but also demonstrates that Danshensu is a potential treatment for COVID-19 patients to inhibit the lung inflammatory response.

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Section: Introductionmentioning

confidence: 99%

Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation

Wang

et al. 2021

Acta Pharmacol Sin

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“…As a routine strategy, bronchodilators and anti‐inflammatory treatments are commonly administered for asthma; however, long‐term administration of glucocorticoids can lead to serious side‐effects (Luo et al., 2019). Nowadays, new therapeutic approaches, such as cell therapy, have attracted the attention of investigators (Mohammadian et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

Human amniotic membrane mesenchymal stem cell‐conditioned medium reduces inflammatory factors and fibrosis in ovalbumin‐induced asthma in mice

Dalouchi

Falak

Bakhshesh³

et al. 2021

Experimental Physiology

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New Findings What is the central question of this study?Is mesenchymal stem cell‐conditioned medium capable of improving the pathological alterations of ovalbumin‐induced asthma in mice? What is the main finding and its importance?Our study indicated that human amniotic membrane mesenchymal stem cell‐conditioned medium is capable of modulating inflammation, fibrosis, oxidative stress and the pathological consequences of ovalbumin‐induced allergic asthma in mice. Abstract Paracrine factors secreted by mesenchymal stem cells (MSCs) have immunomodulatory, anti‐inflammatory and antifibrotic properties, and the conditioned medium (CM) of these cells might have functional capabilities. We examined the effects of human amniotic membrane MSC‐CM (hAM‐MSC‐CM) on ovalbumin (OVA)‐induced asthma. Forty male Balb/c mice were randomly divided into the following four groups: control; OVA (sensitized and challenged with OVA); OVA+CM (sensitized and challenged with OVA and treated with hAM‐MSC‐CM); and OVA+Placebo (sensitized and challenged with OVA and treated with placebo). Forty‐eight hours after the last challenge, serum and bronchoalveolar lavage fluid samples were collected and used for evaluation of inflammatory factors and cells, respectively. Lung tissue sections were stained with Haematoxylin and Eosin or Masson's Trichrome to evaluate pathological changes, and oxidative stress was assessed in fresh lung tissues. Treatment with hAM‐MSC‐CM significantly hindered histopathological changes and fibrosis and reduced the total cell count and the percentage of eosinophils and neutrophils in bronchoalveolar lavage fluid. Furthermore, it reduced serum levels of immunoglobulin E, interleukin‐4, transforming growth factor‐β and lung malondialdehyde. It also increased serum levels of interferon‐γ and interleukin‐10, in addition to the enzymatic activity of glutathione peroxidase, catalase and superoxide dismutase in lung tissue in comparison to the OVA and OVA+Placebo groups. This study showed that administration of hAM‐MSC‐CM can improve pathological conditions, such as inflammation, fibrosis and oxidative stress, in OVA‐induced allergic asthma.

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“…Thus, phenolic compounds including flavonoids are desirable in inhibiting the progression of inflammation due to their antioxidant activity and anti-inflammatory activity. In particular, rosmarinic acid, mostly existing in rosemary and ethanolic extracts obtained from plants, was found to exert anti-inflammatory effects on cells, possibly through inactivation of enzymes such as 5-lipoxygenase, inducible nitric oxide synthase and cyclooxygenase-2 (COX2), and signal proteins such as TNF-α, mitogen-activated protein kinases (MAPKs), nuclear factor kapper B (NF-kB), E-cadherin, vimentin, p38, c-Jun NH 2 -terminal kinase 1/2 (JNK1/2) and Janus kinase 2 (JAK2), as well as signal transducers and activators of transcription 1/3 (STAT1/3) [22][23][24]. Interestingly, previous studies found that perilla extract or rosmarinic acid obviously decreased the degree of gene expression of ICAM-1 and COX-2 in 12-tetradecanoylphorbol 13-acetate treated animals, suggesting anti-inflammatory and ROS scavenging effects [25,26].…”

Section: Discussionmentioning

confidence: 99%

Extracts of ThaiPerilla frutescensnutlets attenuate tumour necrosis factor-α-activated generation of microparticles, ICAM-1 and IL-6 in human endothelial cells

et al. 2020

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Elevation of endothelial microparticles (EMPs) play an important role in the progression of inflammation-related vascular diseases such as cardiovascular diseases (CVDs). Thai perilla (Perilla frutescens) nutlets are rich in phenolic compounds and flavonoids that exert potent antioxidant and anti-inflammatory effects. We found that the ethyl acetate (EA) and ethanol (Eth) extracts of Thai perilla nutlets contain phenolic compounds such as luteolin, apigenin, chryseoriol and their glycosides, which exhibit antioxidant activity. The goal of the present study was to investigate the effects of the extracts on endothelial activation and EMPs generation in tumour necrosis factor-α (TNF-α)-induced EA.hy926 cells. We found that TNF-α (10 ng/ml) activated EA.hy926 cells and subsequently generated EMPs. Pre-treatment with the extracts significantly attenuated endothelial activation by decreasing the expression of the intracellular adhesion molecule-1 (ICAM-1) in a dose-dependent manner. Only the Eth extract showed protective effects against overproduction of interleukin-6 (IL-6) in the activated cells. Furthermore, the extracts significantly reduced TNF-α-enhanced EMPs generation in a dose-dependent manner. In conclusion, Thai perilla nutlet extracts, especially the Eth extract, may have potential to protect endothelium against vascular inflammation through the inhibition of endothelial activation and the generation of endothelial microparticles (EMPs).

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Protective effects and active ingredients of Salvia miltiorrhiza Bunge extracts on airway responsiveness, inflammation and remodeling in mice with ovalbumin-induced allergic asthma

Cited by 40 publications

References 22 publications

Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation

Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation

Human amniotic membrane mesenchymal stem cell‐conditioned medium reduces inflammatory factors and fibrosis in ovalbumin‐induced asthma in mice

Extracts of ThaiPerilla frutescensnutlets attenuate tumour necrosis factor-α-activated generation of microparticles, ICAM-1 and IL-6 in human endothelial cells

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