Protective Effects and Mechanisms of G5 PAMAM Dendrimers against Acute Pancreatitis Induced by Caerulein in Mice

Tang, Yin; Han, Yingchun; Liu, Lu; Shen, Wenwen; Zhang, Huayu; Wang, Yunan; Cui, Xin; Wang, Yuhui; Liu, George; Qi, Rong

doi:10.1021/bm501390d

Cited by 39 publications

(39 citation statements)

References 40 publications

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“…SAP models were prepared according to the protocol by Tang et al[17]. The mice were deprived of food but allowed access to water 24 h prior to the start of the experiments.…”

Section: Methodsmentioning

confidence: 99%

IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitisin vitro

Sun¹,

He²,

Qu³

et al. 2016

WJG

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AIMTo investigate the role of interferon regulatory factor 5 (IRF5) in reversing polarization of lung macrophages during severe acute pancreatitis (SAP) in vitro.METHODSA mouse SAP model was established by intraperitoneal (ip) injections of 20 μg/kg body weight caerulein. Pathological changes in the lung were observed by hematoxylin and eosin staining. Lung macrophages were isolated from bronchoalveolar lavage fluid. The quantity and purity of lung macrophages were detected by fluorescence-activated cell sorting and evaluated by real-time polymerase chain reaction (RT-PCR). They were treated with IL-4/IRF5 specific siRNA (IRF5 siRNA) to reverse their polarization and were evaluated by detecting markers expression of M1/M2 using RT-PCR.RESULTSSAP associated acute lung injury (ALI) was induced successfully by ip injections of caerulein, which was confirmed by histopathology. Lung macrophages expressed high levels of IRF5 as M1 phenotype during the early acute pancreatitis stages. Reduction of IRF5 expression by IRF5 siRNA reversed the action of macrophages from M1 to M2 phenotype in vitro. The expressions of M1 markers, including IRF5 (S + IRF5 siRNA vs S + PBS, 0.013 ± 0.01 vs 0.054 ± 0.047, P < 0.01), TNF-α (S + IRF5 siRNA vs S + PBS, 0.0003 ± 0.0002 vs 0.019 ± 0.018, P < 0.001), iNOS (S + IRF5 siRNA vs S + PBS, 0.0003 ± 0.0002 vs 0.026 ± 0.018, P < 0.001) and IL-12 (S + IRF5 siRNA vs S + PBS, 0.000005 ± 0.00004 vs 0.024 ± 0.016, P < 0.001), were decreased. In contrast, the expressions of M2 markers, including IL-10 (S + IRF5 siRNA vs S + PBS, 0.060 ± 0.055 vs 0.0230 ± 0.018, P < 0.01) and Arg-1 (S + IRF5 siRNA vs S + PBS, 0.910 ± 0.788 vs 0.0036 ± 0.0025, P < 0.001), were increased. IRF5 siRNA could reverse the lung macrophage polarization more effectively than IL-4.CONCLUSIONTreatment with IRF5 siRNA can reverse the pancreatitis-induced activation of lung macrophages from M1 phenotype to M2 phenotype in SAP associated with ALI.

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“…SAP models were prepared according to the protocol by Tang et al[17]. The mice were deprived of food but allowed access to water 24 h prior to the start of the experiments.…”

Section: Methodsmentioning

confidence: 99%

IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitisin vitro

Sun¹,

He²,

Qu³

et al. 2016

WJG

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“…In particular, PAMAM dendrimers show anti-inflammatory activities via different mechanisms determined by surface groups. 46, 47 Neutral dendrimers have been found to target inflammatory cells within the brain. They can localize in activated microglia and astrocytes in the presence of neuroinflammation.…”

Section: Discussionmentioning

confidence: 99%

Targeted nanosystems: Advances in targeted dendrimers for cancer therapy

Yang

2016

Nanomedicine: Nanotechnology, Biology and Medicine

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Dendrimers possess discrete highly compact nanostructures constituted of successive branched layers. Soon after the inception of dendrimers, recognition of their tunable structures and biologically favorable properties provoked a great enthusiasm in delving deeply into the utility of dendrimers for biomedical and pharmaceutical applications. One of the most important nanotechnology applications is the development of nanomedicines for targeted cancer therapies. Tremendous success in targeted therapies has been achieved with the use of dendrimer-based nanomedicines. This article provides a concise review on latest advances in the utility of dendrimers in immunotherapies and hormone therapies.

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“…The nuclear proteins, cytoplasmic proteins, and total proteins of HEp-2 cells were, respectively, extracted according to previously reported methods with minor modification [13]. Protein expression was detected by Western blott under the same conditions as in our previous study [9].…”

Section: Protein Extraction and Western Blotmentioning

confidence: 99%

The Telomerase and Alternative Lengthening of Telomeres Mechanisms Regulate Laryngeal Cancer Cell Apoptosis via the PI3K/Akt Pathway

Wang

Sheng

Cai³

et al. 2018

ORL

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Purpose: To investigate the possible telomerase and alternative lengthening of telomeres (ALT) mechanisms influencing the apoptosis of laryngeal squamous cells. Materials and Methods: The effects of the telomerase mechanism were observed by knockdown of human telomerase reverse transcriptase (hTERT). The ALT mechanism was induced by silencing related genes including TRF2, RAD51, and NBS1. Effects of telomerase and ALT mechanisms on tumor development were confirmed by xenograft tumors model. Tumor cell apoptosis was investigated by flow cytometry and Hoechst staining. Caspase-3 activity assay and Western blot were performed to investigate the possible mechanisms. Results: After silencing ALT- and telomerase mechanism-related genes, Bax and Bcl-2 were increased, and nuclear factor (NF)-κB translocation and PI3K/Akt phosphorylation were inhibited. Conclusions: The inhibition of telomere-related genes inhibited the growth of laryngeal squamous cell carcinoma by promoting cell apoptosis via the PI3K/Akt pathway.

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Protective Effects and Mechanisms of G5 PAMAM Dendrimers against Acute Pancreatitis Induced by Caerulein in Mice

Cited by 39 publications

References 40 publications

IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitisin vitro

IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitisin vitro

Targeted nanosystems: Advances in targeted dendrimers for cancer therapy

The Telomerase and Alternative Lengthening of Telomeres Mechanisms Regulate Laryngeal Cancer Cell Apoptosis via the PI3K/Akt Pathway

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