Protective Effects of 2-Methoxyestradiol on Acute Isoproterenol-Induced Cardiac Injury in Rats

Hareeri, Rawan H.; Alam, Abdulrahman M.; Bagher, Amina M.; Alamoudi, Abdulmohsin J.; Aldurdunji, Mohammed M.; Shaik, Rasheed A.; Eid, Basma G.; Ashour, Osama M.

doi:10.1016/j.jsps.2023.101787

Cited by 5 publications

(3 citation statements)

References 79 publications

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“…CC3 is crucial for the terminal phase of apoptosis [49], and the percentage of CC3-positive cells in the conducted study was increased in the myocardium of animals treated with isoprenaline. Other studies on isoprenalineinduced myocardial injury obtained similar results [41,42,48]. In addition, the results of our study showed a strong positive correlation between NF-κB and CC3.…”

Section: Discussionsupporting

confidence: 89%

“…Similar results were presented by Verma et al, who used the same experimental model of isoprenaline-induced myocardial injury [42]. There is also evidence supporting these findings, which confirmed increased expression of BAX mRNA in isoprenaline-treated rats [48]. The conducted study revealed a positive correlation between NF-κB and BAX as evidence of the involvement of NF-κB in the apoptotic pathway in experimental TTS.…”

Section: Discussionsupporting

confidence: 88%

“…In addition, the results of our study showed a strong positive correlation between NF-κB and CC3. On the other hand, BCL-2, as an antiapoptotic marker, was reduced in isoprenaline-induced myocardial injury, like in other studies [ 42 , 48 ]. During the inhibition of apoptosis, the BCL/BAX ratio is elevated, which contributes to better cell survival [ 50 ].…”

Section: Discussionsupporting

confidence: 82%

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Liraglutide Protects Cardiomyocytes against Isoprenaline-Induced Apoptosis in Experimental Takotsubo Syndrome

Bajic,

Sobot,

Amidzic

et al. 2024

Biomedicines

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Takotsubo syndrome (TTS) is a stress-induced cardiomyopathy, characterized by an increased concentration of catecholamines, free radicals, and inflammatory cytokines, endothelial dysfunction, and increased apoptotic activity. High doses of isoprenaline are used in animal models to induce Takotsubo (TT)-like myocardial injury. The aim of the study was to investigate the antiapoptotic effects of liraglutide in experimental TTS and its role in the NF-κB pathway. Wistar rats were pretreated with liraglutide for 10 days, and on days 9 and 10, TT-like myocardial injury was induced with isoprenaline. After the sacrifice on day 11, hearts were isolated for histopathological and immunohistochemical analysis. Liraglutide reduced isoprenaline-induced cardiomyocyte apoptosis by decreasing cleaved caspase-3 (CC3), BCL-2-associated X protein (BAX), and NF-κB and increasing B-cell lymphoma/leukemia-2 (BCL-2). An increase in NF-κB in isoprenaline-treated rats was in positive correlation with proapoptotic markers (BAX and CC3) and in negative correlation with antiapoptotic marker BCL-2. Liraglutide increased BCL-2 and decreased NF-κB, BAX, and CC3, preserving the same correlations of NF-κB to apoptotic markers. It is concluded that liraglutide protects cardiomyocytes against isoprenaline-induced apoptosis in experimental TT-like myocardial injury through downregulation of the NF-κB pathway.

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