Protective Effects of Alpha-lipoic Acid and Melatonin Against Cadmium-induced Oxidative Stress in Erythrocytes of Rats

Hussei, Samy Ali; Omnia,; El-Ham, M. Abd; F, Ahmad M. Sabry

doi:10.3923/jpt.2014.1.24

Cited by 16 publications

(11 citation statements)

References 68 publications

(47 reference statements)

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“…On the other hand, abovementioned enzyme levels decreased due to Mlt administration in Mlt and Cd + Mlt group animals in present study. These results were consistent with Alabbassi et al (2008) and Hussein et al (2014). Besides, T.pro and Alb levels were found the lowest in Cd group in this study.…”

Section: Discussionsupporting

confidence: 94%

Kadmiyuma Maruz Kalan Ratlarda Oral Melatonin Uygulamasının Oksidatif Stres ve Bağırsak Mikroflorası Üzerine Etkisi

Tavşanlı

Kısadere²

2020

Van Veterinary Journal

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The aim of the present study was to evaluate the effects of oral melatonin treatment on oxidative stress and intestinal microflora in rats exposed to chronic cadmium. Healthy 32 adult male albino Wistar rats were randomly divided into four groups as control (C; n=8), cadmium (Cd; n=8), melatonin (Mlt, n=8), cadmium + melatonin (Cd + Mlt; n=8). Mlt (100 mg/kg) was orally administered 5 times (Mlt and Cd + Mlt), and CdCl2 (2 mg/day) 3 times a week for 4 weeks to rats (Cd and Cd + Mlt). After the treatments, serum total antioxidant status (TAS), total oxidant (TOS) levels as well as plasma ALT, AST, GGT, T.pro, Alb and BUN values were measured. Intestinal contents were aseptically collected, and Enterobacteriaceae, Lactococcus spp. and Lactobacillus spp. counts were performed. As a result, serum TOS levels were defined higher in Cd group than other groups (P <0.05). Lactococcus spp. counts decreased as 0.63 log in Cd group compared to C in small intestine, however it increased as 1.15 log in Cd + Mlt group. In conclusion, Cd + Mlt chelate was found benefical for intestinal microflora due to suppressed the Enterobacteriaceae growth, however ameliorated the Cd induced oxidative stress and Lactobacillus spp./Lactococcus spp. rates in the different part of the intestine.

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Section: Discussionsupporting

confidence: 94%

Kadmiyuma Maruz Kalan Ratlarda Oral Melatonin Uygulamasının Oksidatif Stres ve Bağırsak Mikroflorası Üzerine Etkisi

Tavşanlı

Kısadere²

2020

Van Veterinary Journal

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“…It was established that lead induce oxidative damage and lipid peroxidation in different organs (Hamadouche et al, 2009). Similar results were observed by Hussein et al, (2014) who revealed that, lead intoxicated rats showed significant increase in erythrocytes MDA concentration when compared with normal control group. Furthermore, Omobowale et al, (2014) found a significant increase in MDA and H2O2 generation in the liver and the erythrocytes following exposure to lead.…”

Section: Discussionsupporting

confidence: 78%

Anti-Oxidative Role Of Lipoic Acid Against Lead-Induced Hepatic Toxicity In Japanese Quails

Ismail¹,

Taha²,

Mandour³

et al. 2017

AJVS

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Key words:Quails; Lead; Lipoic acid; Antioxidant; Malondialdehyde, Copper Quails are exposed to deleterious doses of lead from different sources which decrease the bird performance, and growth with economic loses. This work aimed to explore the antioxidative effect of alpha lipoic acid to counteract the adverse effects of lead in Japanese quails. Forty Japanese quails with both sex were grouped into control, lead acetate (100 ppm in drinking water), alpha lipoic acid (1 g/kg diet), and the final group which received both lipoic acid and lead acetate of the same doses. Lead acetate significantly increased the liver enzymatic biomarkers (ALT, AST, and ALP), and bilirubin levels with decline in the serum total protein and albumin concentrations. Also, lead, copper concentrations in liver tissue were significantly increased with decreased hepatic iron level and serum calcium and phosphorus levels following lead acetate administration. Moreover, lead significantly increased hepatic malondialdehyde level with attenuation of antioxidant enzymes; GPX, GST, CAT, and SOD activities and nonenzymatic antioxidant; GSH concentration. Co-administration of alpha-lipoic acid together with lead acetate significantly improved the liver functions, increased the antioxidant activities, and corrected the serum and hepatic major and trace element concentrations without any effect on hepatic lead level. Our results suggested that alpha lipoic acid can counteract the deleterious effects of lead in quails through attenuation of oxidative stress and enhancement of antioxidative capacity in liver tissues.Correspondence to:

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“…LA is a water-and fat-soluble, sulfur-containing compound proven as a metal chelator (Al-Majed et al, 2002). Previous studies have demonstrated that LA has a protective effect on Cd-induced oxidative stress (Veljkovic et al, 2012;Hussein et al, 2014), however, the underlying mechanism is not clear.…”

Section: Introductionmentioning

confidence: 99%

Alpha-lipoic acid protects against cadmium-induced hepatotoxicity via calcium signalling and gap junctional intercellular communication in rat hepatocytes

Zou

Han

et al. 2015

J. Toxicol. Sci.

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-This study investigated the protective effect of alpha-lipoic acid (LA) on cadmium (Cd)-induced hepatotoxicity in BRL 3A rat liver cells. We demonstrated that LA ameliorated Cd-induced cellular injury in cell viability and nuclear fragmentation in BRL 3A cells. Furthermore, LA markedly ameliorated Cd-induced gap junctional intercellular communication (GJIC) inhibition and Cx43 mRNA expression decrease, as well as disassembly of gap junctions. The gap junction blocker carbenoxolone disodium (CBX) as well as LA protected healthy cells from Cd-exposed cells in Transwell co-culture system. LA also protected BRL 3A cells against Cd-induced elevation of the intracellular concentration of free calcium ([Ca 2+ ] i ). Pretreatment with a chelater of intracellular Ca 2+ BAPTA-AM or chelater of extracellular Ca 2+ EGTA attenuated Cd-induced cytotoxicity and GJIC inhibition. CBX exacerbated the decrease in cell viability and further elevated the increase in [Ca 2+ ] i induced by Cd, whereas BAPTA-AM partly attenuated these phenomena, while EGTA had little effects. These results suggested that Cd-induced hepatotoxicity via GJIC inhibition and [Ca 2+ ] i elevation, which originates mainly from intracellular stores. GJIC inhibition has dual effects: (i) it restricts release of Ca 2+ from the cell, which exacerbates the [Ca 2+ ] i elevation and cytotoxicity induced by Cd; and (ii) it protects healthy cells from their dangerous neighbors by blocking intercellular communication. Above all, our results indicated that LA partly prevented Cd-induced cytotoxicity via GJIC and calcium signaling in BRL 3A rat liver cells.

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Protective Effects of Alpha-lipoic Acid and Melatonin Against Cadmium-induced Oxidative Stress in Erythrocytes of Rats

Cited by 16 publications

References 68 publications

Kadmiyuma Maruz Kalan Ratlarda Oral Melatonin Uygulamasının Oksidatif Stres ve Bağırsak Mikroflorası Üzerine Etkisi

Kadmiyuma Maruz Kalan Ratlarda Oral Melatonin Uygulamasının Oksidatif Stres ve Bağırsak Mikroflorası Üzerine Etkisi

Anti-Oxidative Role Of Lipoic Acid Against Lead-Induced Hepatic Toxicity In Japanese Quails

Alpha-lipoic acid protects against cadmium-induced hepatotoxicity via calcium signalling and gap junctional intercellular communication in rat hepatocytes

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