Protective effects of andrographolide against diclofenac-induced gastric damage

Tandoh, Augustine; Danquah, Cynthia Amaning; Ossei, Paul Poku Sampene; Benneh, Charles Kwaku; Ayibor, William Gilbert; Woode, Eric

doi:10.1016/j.sciaf.2021.e00944

Cited by 5 publications

(8 citation statements)

References 22 publications

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“…These findings agree with the findings of (Mostafa, R.E. et al, 2020); and (Tandoh, A. et al, 2021), these studies show that diclofenac does increases lesions number and severity significantly due to the mechanisms mentioned above. 17,18 The increased levels of MPO and MDA in the stomach of diclofenac-ulcerated rats is a symptom of accelerated lipid peroxidation and free radical overproduction, resulting in mucosal injury, according to the current study which came in agree with (Akinrinde, A.S. and Hameed, H.O., 2022).…”

Section: Discussionsupporting

confidence: 93%

“…The macroscopic appearance of these mucosal lesions is in accordance with the finding, in which they expressed a very similar gastric mucosal damage with close features for this current study. 17,18 The present study revealed that there is a significant increase in the lesion number and lesion severity (which is represented by the total linear lengths of lesions). in ulcerated rats following diclofenac sodium oral administration (150 mg/kg).…”

Section: Discussionmentioning

confidence: 48%

“…et al, 2020); and (Tandoh, A. et al, 2021), these studies show that diclofenac does increases lesions number and severity significantly due to the mechanisms mentioned above. 17,18 The increased levels of MPO and MDA in the stomach of diclofenac-ulcerated rats is a symptom of accelerated lipid peroxidation and free radical overproduction, resulting in mucosal injury, according to the current study which came in agree with (Akinrinde, A.S. and Hameed, H.O., 2022). 19 In which they expressed an elevation of serum MPO activity (83.30%), when compared with control, and also in agreement with (Fornai, M. et al, 2020) 20 in which they expressed increments of MPO and MDA, overexpression.…”

Section: Discussionmentioning

confidence: 86%

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The Potential Protective Eff ects of Pirfenidone on Diclofenac Sodium Induced Gastric Ulcer in a Rat Model

Abdelfatah¹,

Al-Qadh²

2023

IJDDT

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Introduction: Non-steroidal anti-infl ammatory drugs (NSAIDs), are widely prescribed in clinical practice because of their ability to reduce pain, fever, and infl ammation. However, NSAID-induced gastric mucosal damage is the major side eff ect of these medications. This study aims to reduce gastric mucosal lesions caused by NSAIDs by using pirfenidone by investigating their eff ect on histological, gastric gross mucosal damage. Method: 30 healthy male albino rats were divided into 3 groups each of ten (N=10). A single oral dose of diclofenac sodium (150 mg/kg body weight) was used to induce ulceration for all groups except fi rst. The vehicle (tween 80+NS) was given to the fi rst group, while diclofenac sodium was given to the second group and to all pre-treated groups. The third group were pre-treated with pirfenidone (300 mg/kg). At the end of the experiment, histological examination, and antioxidant and antiinfl ammatory parameters by immunohistochemistry method were evaluated. Results: Diclofenac sodium at a dose (150 mg/kg) produces a signifi cant increment (p > 0.01) in gastric damage score, the expression of tumor necrosis factor-alpha (TNF-α), myeloperoxidase, malonaldehyde, and the ulcer formation percent, compared to the healthy rat’s group. Pirfenidone at a dose of (300 mg/kg) pretreatment in diclofenac induced-ulcer in rats produces a signifi cant reduction (p > 0.01) in gastric damage score, the expression of TNF-alpha, myeloperoxidase, the expression of TNF-alpha, myeloperoxidase, malonaldehyde, and in the ulcer formation percent, yet, less eff ectively than omeprazole and pirfenidone. Conclusion: Diclofenac can be reduced or prevented by the pretreatment of pirfenidone. Pirfenidone showed similar results to the standard treatment (Omeprazole), the protective eff ect of pirfenidone was mainly through their antioxidant and anti-infl ammatory activity by reducing oxidation markers like MPO and MDA, and also reducing infl ammatory cytokines like TNF-alpha

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 86%

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The Potential Protective Eff ects of Pirfenidone on Diclofenac Sodium Induced Gastric Ulcer in a Rat Model

Abdelfatah¹,

Al-Qadh²

2023

IJDDT

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“…Natural phytochemicals protect sunlight‐induced cellular and molecular changes via signal transduction pathways 15 . Andrographolide (ADP), a bicyclic diterpenoid lactone, is the principal constituent of the medicinal plant Andrographis paniculate 16,17 .…”

Section: Introductionmentioning

confidence: 99%

Preventive effect of andrographolide against ultraviolet‐B radiation‐induced oxidative stress and apoptotic signaling in human dermal fibroblasts

Indirapriyadarshini,

Radhiga,

Kanimozhi

et al. 2023

Cell Biochemistry & Function

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Ultraviolet radiation induces oxidative photoaging in the skin cells. In this study, we investigated the ability of andrographolide (ADP) to protect human dermal fibroblasts (HDFa) from UVB radiation‐induced oxidative stress and apoptosis. The HDFa cells were exposed to UVB (19.8 mJ/cm2) radiation in the presence or absence of ADP (7 μM) and then oxidative stress and apoptotic protein expression were analyzed. UVB exposure resulted in a significant decline in the activity of antioxidant enzymes and altered mitochondrial membrane potential (MMP). Furthermore, UVB‐irradiation causes increased intracellular reactive oxygen species (ROS) production, apoptotic morphological changes, and lipid peroxidation levels in the HDFa. Moreover, the pretreatment with ADP reduced the UVB‐induced cytotoxicity, ROS production, and increased antioxidant enzymes activity. Further, the ADP pretreatment prevents the UVB‐induced loss of MMP and apoptotic signaling in HDFa cells. Therefore, the present results suggest that ADP protects HDFa cells from UVB‐induced oxidative stress and apoptotic damage.

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“…This ancient medicinal plant has an extensive ethnobotanical history and can be easily found in South-East Asia, China and India. The established pharmacological effects of A. paniculata extract are anticancer and immunostimulatory activity [1,2], antimalarial [3,4], antiinflammatory [5], antioxidative [6], antimicrobial [7], antiviral [8,9], respiratory system benefits [10], neuroprotective [11], gastroprotective [12], hepatoprotective activity [13]. Due to its versatile bioactivities, A. paniculata was included in the World Health Organization (WHO) monograph of 2002 as an endorsed medicinal plant [14].…”

Section: Introductionmentioning

confidence: 99%

Probe Ultrasonic-Assisted Extraction of Andrographolide Rich Extract from Andrographis paniculata: Processing Parameters Optimization Using Response Surface Methodology

et al. 2022

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The conventional extraction method has low extraction efficiency. This study aims to optimize the ultrasonic-assisted extraction conditions of andrographolide from A. paniculata. A ½ inch ultrasonic probe sonicator was used to extract A. paniculata. The effects of extraction time (X1), duty cycle (X2) and amplitude (X3) on the yield of andrographolide (Y) were investigated by using response surface methodology. Experiments were designed according to 3-level 3-factor Box Behnken Design. The concentrations of andrographolide were quantified by high-performance liquid chromatography. Results of analysis of variance showed the data were well fitted with the 2nd-order polynomial equation with a R2 value of 0.9969 and p-value less than 0.005. Optimum extraction conditions were found to be at 5 min, 11 % duty cycle, and 66 A. A checkpoint experiment was carried out based on the optimum conditions, validated highest andrographolide was 3.50 ± 0.17 w/w%. Response surface methodology was successfully applied to optimize the extraction processing parameters for yielding the andrographolide-rich A. paniculata extract. This study could be relevant for future ultrasonic-assisted extraction scale-up of andrographolide. HIGHLIGHTS Andrographis paniculata was extracted using ultrasonic probe sonicator Variables studied were time, duty cycle, and amplitude Extraction parameters were sucessfully optimized by response surface methodology 50 ± 0.17 w/w% of andrographolide was extracted. GRAPHICAL ABSTRACT

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Protective effects of andrographolide against diclofenac-induced gastric damage

Cited by 5 publications

References 22 publications

The Potential Protective Eff ects of Pirfenidone on Diclofenac Sodium Induced Gastric Ulcer in a Rat Model

The Potential Protective Eff ects of Pirfenidone on Diclofenac Sodium Induced Gastric Ulcer in a Rat Model

Preventive effect of andrographolide against ultraviolet‐B radiation‐induced oxidative stress and apoptotic signaling in human dermal fibroblasts

Probe Ultrasonic-Assisted Extraction of Andrographolide Rich Extract from Andrographis paniculata: Processing Parameters Optimization Using Response Surface Methodology

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