Protective effects of Antarctic krill oil in dextran sulfate sodium-induced ulcerative colitis mice

Zhou, Xiaoling; Xiang, Xingwei; Zhou, Yufang; Zhou, Tianyi; Deng, Shanggui; Zheng, Bin; Zheng, Pingan

doi:10.1016/j.jff.2021.104394

Cited by 30 publications

(36 citation statements)

References 68 publications

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“…Additionally, DAO and LPS levels in the intestine and serum reflect disruption of the intestinal mechanical barrier ( 32 ). Immunogenic endotoxins such as LPS are produced by the intestinal microbiota and released from the gut into the blood, exacerbating the inflammatory response during circulation ( 20 ). DAO is a strongly reactive intracellular enzyme present in mammalian intestinal epithelial cells and is released into the intestinal tract and bloodstream as the intestinal epithelial cells are stripped and begin to disintegrate ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

“…Immunogenic endotoxins such as LPS are produced by the intestinal microbiota and released from the gut into the blood, exacerbating the inflammatory response during circulation ( 20 ). DAO is a strongly reactive intracellular enzyme present in mammalian intestinal epithelial cells and is released into the intestinal tract and bloodstream as the intestinal epithelial cells are stripped and begin to disintegrate ( 20 ). In this study, DAO and LPS levels in the colon and serum of mice in all SP-treated groups decreased significantly compared with those in the model group ( P < 0.05), indicating that SP could reduce intestinal mucosal damage and help restore the intestinal mucosal barrier.…”

Section: Discussionmentioning

confidence: 99%

“…Based on our previously reported method ( 20 ), in this study, 32 four-week-old male ICR mice were purchased from Shanghai Slack Laboratory Animal Co., Ltd. All mice were fed a standard diet for 1 week in an animal room and were subjected to a 12 h/12 h dark/light cycle, a temperature of 24 ± 1°C, and humidity of 60 ± 5%. At the end of the adaptation period, the mice were randomly divided into 4 groups (8 mice per group).…”

Section: Methodsmentioning

confidence: 99%

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Protective Effects of Shrimp Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

et al. 2021

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Inflammatory bowel disease, an intestinal relapsing inflammatory disease, not only impairs gastrointestinal function but also increases the chances of developing colon cancer. Currently, the effects of shrimp peptide (SP) in mice model of ulcerative colitis (UC) are still unclear. In particular, it is uncertain whether SP affects the gut flora with UC mice. In this study, we investigated the anti-inflammatory effects of SP on a dextran sulfate sodium (DSS)-induced mouse model of UC. Firstly, the molecular weight of SP was mainly distributed in the range of 180–1,000 Da (61.95% proportion), and the amino acid composition showed that SP contained 17 amino acids, of which, the essential amino acids accounted for 54.50%. In vivo, oral SP significantly attenuated the severity of colitis, such as diarrhea, weight loss, and rectal bleeding. Furthermore, treatment with SP remarkably ameliorated intestinal barrier integrity, thus lowering the levels of the inflammatory cytokines and ameliorating antioxidant indices and intestinal injury indicators in the serum and colon. Lastly, the cecal contents were used to sequence and analyze the 16S rRNA genes of bacteria. Results suggested that treatment with SP could restore the balance of intestinal flora in modeled mice by regulating the abundance of pathogenic and beneficial bacteria. Furthermore, SP could significantly improve intestinal flora dysfunction in mice with UC. In summary, our findings show that SP has a prophylactic and therapeutic effect in UC in vivo, thereby highlighting its broad medicinal applications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Protective Effects of Shrimp Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

et al. 2021

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“…Its total biomass is up to 6.5–10 million tons and has been thought as the largest underexploited resource of the ocean [ 3 , 4 ]. Presently, Antarctic krill is thought as an abundant and high-quality resource for various food and health-care products because some bioactive substances, including oil, peptides and protein, astaxanthin, and chitin, have been prepared from Antarctic krill ( E. superba ) and its processing by-products [ 5 , 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

et al. 2021

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For utilizing the largest source of marine proteins, Antarctic krill (Euphausia superba) proteins were defatted and hydrolyzed separately using pepsin, alcalase, papain, trypsin, and netrase, and alcalase hydrolysate (EPAH) showed the highest DPPH radical (DPPH·) and hydroxyl radical (HO·) scavenging activity among five hydrolysates. Using ultrafiltration and chromatography methods, fifteen antioxidant peptides were purified from EPAH and identified as Asn-Gln-Met (NQM), Trp-Phe-Pro-Met (WFPM), Gln-Asn-Pro-Thr (QNPT), Tyr-Met-Asn-Phe (YMNF), Ser-Gly-Pro-Ala (SGPA), Ser-Leu-Pro-Tyr (SLPY), Gln-Tyr-Pro-Pro-Met-Gln-Tyr (QYPPMQY), Glu-Tyr-Glu-Ala (EYEA), Asn-Trp-Asp-Asp-Met-Arg-Ile-Val-Ala-Val (NWDDMRIVAV), Trp-Asp-Asp-Met-Glu-Arg-Leu-Val-Met-Ile (WDDMERLVMI), Asn-Trp-Asp-Asp-Met-Glu-Pro-Ser-Phe (NWD-DMEPSF), Asn-Gly-Pro-Asp-Pro-Arg-Pro-Ser-Gln-Gln (NGPDPRPSQQ), Ala-Phe-Leu-Trp-Asn (AFLWA), Asn-Val-Pro-Asp-Met (NVPDM), and Thr-Phe-Pro-Ile-Tyr-Asp-Tyr-Pro-Gln (TFPIYDPQ), respectively, using a protein sequencer and ESI/MS. Among fifteen antioxidant peptides, SLPY, QYPPMQY and EYEA showed the highest scavenging activities on DPPH· (EC50 values of 1.18 ± 0.036, 1.547 ± 0.150, and 1.372 ± 0.274 mg/mL, respectively), HO· (EC50 values of 0.826 ± 0.027, 1.022 ± 0.058, and 0.946 ± 0.011 mg/mL, respectively), and superoxide anion radical (EC50 values of 0.789 ± 0.079, 0.913 ± 0.007, and 0.793 ± 0.056 mg/mL, respectively). Moreover, SLPY, QYPPMQY and EYEA showed strong reducing power, protective capability against H2O2-damaged plasmid DNA, and lipid peroxidation inhibition ability. Furthermore, SLPY, QYPPMQY, and EYEA had high stability under temperatures lower than 80 °C, pH values ranged from 6–8, and simulated GI digestion for 180 min. The results showed that fifteen antioxidant peptides from alcalase hydrolysate of Antarctic krill proteins, especially SLPY, QYPPMQY and EYEA, might serve as effective antioxidant agents applied in food and health products.

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“…The content of DAO and LPS in the intestine and blood incarnate the integrity of the intestinal mechanical barrier and the degree of damage [ 41 ]. The endotoxin produced by the bacteria represented by Gram-negative bacteria will leak out from the intestine into the blood and participate in the blood circulation of the body, intensifying the inflammatory response [ 42 ]. DAO is a hugely active intracellular enzyme mainly found in intestinal epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice

et al. 2021

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Considerable literature has been published on polysaccharides, which play a critical role in regulating the pathogenesis of inflammation and immunity. In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated. The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway. For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum. Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora. Specifically, mice treated with MP showed a repaired Firmicutes/Bacteroidetes ratio and an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon. These data suggest that the MP could be a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis.

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Protective effects of Antarctic krill oil in dextran sulfate sodium-induced ulcerative colitis mice

Cited by 30 publications

References 68 publications

Protective Effects of Shrimp Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

Protective Effects of Shrimp Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice

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