Protective effects of atorvastatin and quercetin on isoprenaline-induced myocardial infarction in rats

Zaafan, Mai A.; Zaki, Hala F.; El-Brairy, Amany I.; Kenawy, Sanaa A.

doi:10.1016/j.bfopcu.2013.03.001

Cited by 49 publications

(38 citation statements)

References 32 publications

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“…In our study, HM group demonstrated a significant ST-segment elevation which could be due to the deleterious effects of isoproterenol on cardiac cell membrane integrity with subsequent reduced mechanical capacity of the ventricles (Thippeswamy et al, 2009). Similar ECG changes were reported in isoproterenol-infarcted (Zaafan et al, 2013) and hypercholesterolemic isoproterenol-infarcted rats (AboGresha et al, 2014).…”

Section: Discussionsupporting

confidence: 87%

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Rosuvastatin and ellagic acid protect against isoproterenol-induced myocardial infarction in hyperlipidemic rats

Elhemely

Omar

Ain-Shoka

et al. 2014

Beni-Suef University Journal of Basic and Applied Sciences

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Hyperlipidemia Isoproterenol RosuvastatinEllagic acid a b s t r a c t Hyperlipidemia (HL) with subsequent coronary atherosclerosis is the major trigger of ischemia and hence, myocardial infarction (MI) occurs. The present study aimed to elucidate the effects of pretreatment with rosuvastatin and ellagic acid, as well as their combination on isoproterenol-induced MI in hyperlipidemic rats. Adult rats were fed with a cholesterol-rich diet for seven weeks and received rosuvastatin (10 mg/kg) and/or ellagic acid (30 mg/kg) by oral gavage daily starting from the fifth week then subcutaneously injected with two doses 24-h apart of 100 mg/kg isoproterenol in the last two days. ECG pattern was monitored and both cardiac biomarkers (cTnI, CK-MB, LDH and AST) and lipid profile (TC, TG, HDL-c and LDL-c) were measured in serum. MDA and GSH levels were quantified in cardiac homogenates and heart tissue damage was examined by histopathology. Furthermore, the expression levels of iNOS, eNOS, Bax and Bcl-2 in heart samples were assessed by western blotting. Three-week pretreatment with rosuvastatin and/or ellagic acid markedly ameliorated HL-and isoproterenol-induced alterations in ECG, cardiac markers, oxidation markers, lipid profile and heart architecture. Both drugs downregulated iNOS and upregulated eNOS, while only rosuvastatin and the combination downregulated Bax. This study provides evidence that rosuvastatin and ellagic acid possess cardioprotective effect on the hyperlipidemic-myocardial infarction rat model and the combination does not offer extra protection than monotherapy.

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Section: Discussionsupporting

confidence: 87%

“…Two doses 24-h apart of 100 mg/kg isoproterenol, prepared in saline, were injected subcutaneously in rats (Zaafan et al, 2013). The injected area was massaged for 30 s after injection.…”

Section: Induction Of MImentioning

confidence: 99%

Rosuvastatin and ellagic acid protect against isoproterenol-induced myocardial infarction in hyperlipidemic rats

Elhemely

Omar

Ain-Shoka

et al. 2014

Beni-Suef University Journal of Basic and Applied Sciences

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“…SERCA was stimulated by low quercetin concentrations (<25 μM), which is close to the one we used in the present study. Another study showed that quercetin decreased isoprenaline‐induced changes in oxidative stress and inflammatory biomarkers with marked improvement in ECG and histopathologic alterations . However, despite the increase in Ca 2+ transient amplitude and higher rates of reuptake, SR Ca 2+ load was found unchanged under acute exposure to quercetin.…”

Section: Discussionmentioning

confidence: 99%

Dissection of the Effects of Quercetin on Mouse Myocardium

Santos

Oliveira

Santos‐Miranda

et al. 2017

Basic Clin Pharma Tox

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Quercetin is a plant flavonoid with several biological activities. This study aimed to describe quercetin effects on contractile and electrophysiological properties of the cardiac muscle as well as on calcium handling. Quercetin elicited positive inotropism that was significantly reduced by propranolol indicating an involvement of the sympathetic nervous system. In cardiomyocytes, 30 μM quercetin increased I at 0 mV from -0.95 ± 0.01 A/F to -1.21 ± 0.08 A/F. The membrane potential at which 50% of the channels are activated (V ) shifted towards more negative potentials from -13.06 ± 1.52 mV to -19.26 ± 1.72 mV and did not alter the slope factor. Furthermore, quercetin increased [Ca ] transient by 28% when compared to control. Quercetin accelerated [Ca ] transient decay time, which could be attributed to SERCA activation. In resting cardiomyocytes, quercetin did not change amplitude or frequency of Ca sparks. In isolated heart, quercetin increased heart rate and decreased PRi, QTc and duration of the QRS complex. Thus, we showed that quercetin activates β-adrenoceptors, leading to increased L-type Ca current and cell-wide intracellular Ca transient without visible changes in Ca sparks.

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“…In this study the SC injection of isoprenaline is used to induce MI, which causes damage as ischemia/reperfusion while in previous studies coronary artery occlusion of the left anterior-descending artery (LAD) is used. Isoprenaline effect mechanism in the cardiac damage, including the production of free radicals, increase in cytosolic calcium overload and mitochondria damage or dysfunction (19). Isoprenaline is a synthetic catecholamine and betaadrenergic receptor agonist that upon oxidation, generates highly toxic free radicals and stimulates membrane phospholipids peroxidation and severe myocardial membrane damage.…”

Section: Discussionmentioning

confidence: 99%

“…Isoprenaline is a synthetic catecholamine and betaadrenergic receptor agonist that upon oxidation, generates highly toxic free radicals and stimulates membrane phospholipids peroxidation and severe myocardial membrane damage. It is reported that high levels of circulating catecholamines cause cardiac toxicity (19,20). Isoprenaline or isoproterenol-induced myocardial injury represents the occurrence of oxidative damage, lipid peroxidation, LV hemodynamic and function changes following hypertrophy, inflammatory cells infiltration, fibrous and tissue necrosis.…”

Section: Discussionmentioning

confidence: 99%

Effect of endurance exercise training on morphological changes in rat heart tissue following experimental myocardial infarction

Jazi

Abdi

Ahmadi

et al. 2017

JBRMS

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Introduction: Cardiac remodeling after myocardial infarction, is associated with progressive ventricular dysfunction and cardiovascular death. The purpose of this study was to examine the effect of endurance exercise training on morphological changes in rat heart tissue following experimental myocardial infarction. Materials and methods: Rats used in this experiment (8-10 weeks old, 235 ± 5 g body weight) after homogenization according to body weight, were randomly divided to three groups: Healthy (Sham), control (Sedentary) and endurance training groups. Myocardial infarction was induced by subcutaneous injection of isoprenaline (150 mg/kg) in two consecutive days with an interval of 24 hours. Endurance training initiated 2 days after infarction and continued for 4 weeks. In order to assess the necrosis lesion and fibrosis tissue, Hematoxylin-Eosin and Masson's trichrome staining were used, respectively. Data were analyzed by using one-way analysis of variance (ANOVA) at P< 0.05 level. Results:The results indicated that subcutaneous injection of isoprenaline, induced myocardial infarction in rat heart tissue. Rate of necrotic lesion in the Endurance training group was significantly higher than Sedentary (control) group (P=0.005).Also, the size of fibrosis tissue in the Endurance training group was significantly higher than the sedentary (control) group (P= 0.001). Conclusion: In general, results of this study showed that 4 weeks of endurance exercise training after myocardial infarction exacerbate tissue injury in rat heart.

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Protective effects of atorvastatin and quercetin on isoprenaline-induced myocardial infarction in rats

Cited by 49 publications

References 32 publications

Rosuvastatin and ellagic acid protect against isoproterenol-induced myocardial infarction in hyperlipidemic rats

Rosuvastatin and ellagic acid protect against isoproterenol-induced myocardial infarction in hyperlipidemic rats

Dissection of the Effects of Quercetin on Mouse Myocardium

Effect of endurance exercise training on morphological changes in rat heart tissue following experimental myocardial infarction

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