Protective Effects of Baicalin on Arsenic Trioxide-induced Oxidative Damage and Apoptosis in Human Umbilical Vein Endothelial Cells

Tsai, Chuen-Tinn; Tsai, Chia-Wen; Chang, Wen-Shin; Lin, Ji-Ping; Hsia, Te‐Chun; Bau, Da‐Tian

doi:10.21873/invivo.12243

Cited by 13 publications

(9 citation statements)

References 38 publications

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“…The subtle alterations in DNA adducts are one of the end points for understanding how ROS induced intracellular damage and cell death. Consistent with previous findings (28), treatment of As 2 O 3 was capable of inducing FPG-digestible endonuclease III-digestible DNA adducts dose-dependently at the range of 4~16 μM (Figure 5 and Figure 6). As previously reported, FPG can specifically digest oxidized purines, such as 8-oxoguanine, 5-hydroxycytosine, 5-hydroxyuracil, 2,6-diamino-4-hydroxy- 5-N-methylformamidopyrimidine, and 4,6-diamino-5formamidopyrimidine (35).…”

Section: Discussionsupporting

confidence: 92%

“…NADPH oxidase activity. The NADPH oxidase activity of HUVEC cells was checked as previously described (28). After drug treatments, HUVEC cells were harvested and centrifuged for 10 min at 4˚C at 10,000 rpm.…”

Section: Methodsmentioning

confidence: 99%

“…Comet assay for oxidative DNA adducts. The FPG-and endonuclease III-digestible adducts of HUVEC cells were examined as previously described (28). Briefly, after preparation of typical 3-layer agarose gel slides, the slides were lysed, washed, and incubated at 37˚C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

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Protective Effects of Crocetin on Arsenic Trioxide-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

Tsai

Chang

et al. 2021

In Vivo

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Background/Aim: The clinical use of arsenic trioxide (As 2 O 3 ) is hampered due to its cardiotoxicity. Therefore, it is critical to prevent As 2 O 3 -induced loss of endothelial integrity. The purpose of this study was to examine As 2 O 3 -induced endothelial dysfunction and evaluate the efficacy of crocetin on reversing As 2 O 3 -induced cardiotoxicity. Materials and Methods: Cultured human umbilical vein endothelial cells (HUVECs) were used to examine As 2 O 3 -induced oxidative stress, apoptosis, production of reactive oxygen species (ROS) and DNA adducts. In addition, the impact of crocetin on As 2 O 3induced cardiotoxicity was evaluated. Results: As 2 O 3 decreased the viability of HUVEC cells and led to apoptosis. Additionally, As 2 O 3 elevated NADPH oxidase activity, and the levels of intracellular ROS. Furthermore, the formamidopyrimidine DNA-glycosylase-and endonuclease III-digestible adducts were induced by As 2 O 3 . Crocetin treatment reversed the As 2 O 3induced reduction in cell viability, the induction of apoptosis, the activation of NADPH oxidase activity, ROS levels and DNA adducts. Conclusion: Crocetin protects from As 2 O 3 -induced cardio-toxicity.Arsenic trioxide (As 2 O 3 ) is a very toxic agent used in Chinese medicine, which has been used to successfully treat refractory acute promyelocytic leukemia (1). The efficacy of As 2 O 3 has been reported for its capacity to induce acute promyelocytic leukemia cells to undergo apoptosis, however, its cardio-toxicity has hindered its therapeutic application (2, 3). In addition, accumulated literature has pointed that chronic exposure to As 2 O 3 from drinking water is closely associated with various human diseases, including atherosclerosis (4), diabetes mellitus (5), hypertension (6), ischemic heart disease (7, 8), peripheral vascular disease (9), and cancer (10,11). Up to date, relatively few published articles are available on the influence of acute or chronic As 2 O 3 exposure on the vascular endothelial system. At the same time, the identification of potential traditional Chinese medicine for the prevention of the diseases associated with chronic exposure to As 2 O 3 is of great interest. Εndothelial cells, human umbilical vein endothelial cells (HUVEC) are frequently used as the cell model, are the main targets of vasculopathy caused by As 2 O 3 exposure. As 2 O 3 has been reported to cause oxidative stress, and induce endothelial cells to undergo programmed cell death (12). Oxidative stress and its intracellular consequences are believed to be the major cause of As 2 O 3 cytotoxicity (13).Crocetin (C 20 H 24 O 4 ; molecular weight 328.4g/mol) is a primary constituent of saffron (Crocus sativus L), which has been known to possess lots of beneficial pharmacological effects, such as anti-inflammatory ( 14) and anti-apoptotic (15, 16). Crocetin has also been reported to effectively eliminate ischemia-reperfusion-induced oxidative damage in rats and scavenge free radicals (17). More interesting, crocetin has beneficial cardiovascular effects, su...

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Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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Protective Effects of Crocetin on Arsenic Trioxide-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

Tsai

Chang

et al. 2021

In Vivo

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“…Baicalin was also found to inhibit the apoptosis of BMECs by reducing the generation of cellular ROS [ 20 ] and attenuated cell damage in the rat pheochromocytoma cell line (PC12) that was induced by hydrogen peroxide [ 23 ]. Arsenic trioxide is an environmental pollutant that can inhibit the viability of human umbilical vein endothelial cells (HUVECs) and promote cell apoptosis, while Baicalin can reverse the toxic effects of arsenic trioxide on HUVECs [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Baicalin improves the functions of granulosa cells and the ovary in aged mice through the mTOR signaling pathway

Fan

Bai

et al. 2022

J Ovarian Res

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Background The mammalian follicle is the basic functional unit of the ovary, and its normal development is required to obtaining oocytes capable of fertilization. As women get older or decline in ovarian function due to certain pathological factors, the growth and development of follicles becomes abnormal, which ultimately leads to infertility and other related female diseases. Kuntai capsules are currently used in clinical practice to improve ovarian function, and they contain the natural compound Baicalin, which is a natural compound with important biological activities. At present, the role and mechanism of Baicalin in the development of ovarian follicles is unclear. Methods Human primary granulosa cells collected from follicular fluid, and then cultured and treated with Baicalin or its normal control, assessed for viability, subjected to RT-PCR, western blotting, flow cytometry, and hormone analyses. The estrus cycle and oocytes of CD-1 mice were studied after Baicalin administration and compared with controls. Ovaries were collected from the mice and subjected to hematoxylin-eosin staining and immunohistochemistry analysis. Results We showed that Baicalin had a dose-dependent effect on granulosa cells cultured in vitro. A low concentration of Baicalin (for example, 10 μM) helped to maintain the viability of granulosa cells; however, at a concentration exceeding 50 μM, it exerted a toxic effect. A low concentration significantly improved the viability of granulosa cells and inhibited cell apoptosis, which may be related to the resultant upregulation of Bcl-2 expression and downregulation of Bax and Caspase 3. By constructing a hydrogen peroxide-induced cell oxidative stress damage model, we found that Baicalin reversed the cell damage caused by hydrogen peroxide. In addition, Baicalin increased the secretion of estradiol and progesterone by upregulating P450arom and stAR. The results of the in vivo experiment showed that the intragastric administration of Baicalin to aged mice improved the estrous cycle and oocyte quality. Furthermore, we observed that Baicalin enhanced the viability of granulosa cells through the mTOR pathway, which in turn improve ovarian function. Conclusion These results indicate that Baicalin could improve the viability of ovarian granulosa cells and the secretion of steroid hormones and thus could help to improve degenerating ovarian function and delay ovarian aging.

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“…Arsenic trioxide (As 2 O 3 ) was chosen since arsenic is one of the most toxic metals derived from the natural environment. The current major cause of human arsenic toxicity is from contamination of drinking water from natural geological sources [ 16 ], and possibly partly upon the medical applications of arsenic trioxide (As 2 O 3 ). Fortunately, the use of arsenical pesticides in agriculture has decreased dramatically [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Joushomme

Garenne

Dufossée

et al. 2022

IJMS

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It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-exposure to RF and chemical treatments known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). The specific absorption rates (S.A.R.) used were 1.5 and 6 W/kg during DHM experiments and ranged from 5 to 24 W/kg during the recording of cellular impedance. Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant.

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Protective Effects of Baicalin on Arsenic Trioxide-induced Oxidative Damage and Apoptosis in Human Umbilical Vein Endothelial Cells

Cited by 13 publications

References 38 publications

Protective Effects of Crocetin on Arsenic Trioxide-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

Protective Effects of Crocetin on Arsenic Trioxide-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

Baicalin improves the functions of granulosa cells and the ovary in aged mice through the mTOR signaling pathway

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

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