Protective Effects of Bee Pollen on Multiple Propionic Acid-Induced Biochemical Autistic Features in a Rat Model

Alfawaz, Hanan; El‐Ansary, Afaf; Al-Ayadhi, Laila; Bhat, Ramesa Shafi; Hassan, Wail M.

doi:10.3390/metabo12070571

Cited by 8 publications

(11 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Long chain fatty acids such as linoleic (Omega-3), α-linolenic (Ω-6), oleic (Ω-9), and α-palmitic acid (saturated) are among the major fatty acids in BP [ 5 , 64 ]. Unsaturated fatty acids may reach 60% of pollen lipids, while palmitic acid is the most abundant saturated fatty acid [ 65 ]. Other fatty acids such as oleic acid and myristic acid have also been reported as the main lipidic components [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World

Kacemi,

Campos

2023

Nutrients

View full text Add to dashboard Cite

The emphasis on healthy nutrition is gaining a forefront place in current biomedical sciences. Nutritional deficiencies and imbalances have been widely demonstrated to be involved in the genesis and development of many world-scale public health burdens, such as metabolic and cardiovascular diseases. In recent years, bee pollen is emerging as a scientifically validated candidate, which can help diminish conditions through nutritional interventions. This matrix is being extensively studied, and has proven to be a very rich and well-balanced nutrient pool. In this work, we reviewed the available evidence on the interest in bee pollen as a nutrient source. We mainly focused on bee pollen richness in nutrients and its possible roles in the main pathophysiological processes that are directly linked to nutritional imbalances. This scoping review analyzed scientific works published in the last four years, focusing on the clearest inferences and perspectives to translate cumulated experimental and preclinical evidence into clinically relevant insights. The promising uses of bee pollen for malnutrition, digestive health, metabolic disorders, and other bioactivities which could be helpful to readjust homeostasis (as it is also true in the case of anti-inflammatory or anti-oxidant needs), as well as the benefits on cardiovascular diseases, were identified. The current knowledge gaps were identified, along with the practical challenges that hinder the establishment and fructification of these uses. A complete data collection made with a major range of botanical species allows more robust clinical information.

show abstract

Section: Resultsmentioning

confidence: 99%

Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World

Kacemi,

Campos

2023

Nutrients

View full text Add to dashboard Cite

show abstract

“…Postural imbalance directly causes the relationship with dopaminergic neuron fluctuation in the striatum. Moreover, impairment in GABA/glutamate hemostasis leads to excitotoxicity, which is a major factor for the progression of disorder and contributes to dysregulating the apoptotic schedule of neurons 12 . Apoptosis mainly occurs via the overactivation of the p38 MAPK pathway, which comprises GSK‐3β expression, resulting in excitotoxicity and impaired motor coordination 13 .…”

Section: Introductionmentioning

confidence: 99%

“…In the embryogenesis of rats, the formation of a neuronal circuit for the cranial nerve occurs on GD 12 or 13 after the closing of the neural tube on GD 11. 11 This time duration (GD [12][13] is crucial for the genesis of several sensory and motor nerves. Disruption in cranial nerve morphology, behavioral impairment, and changes in relevant gene expression have been observed following VPA exposure during pregnancy at particular time window.…”

mentioning

confidence: 99%

“…Moreover, impairment in GABA/glutamate hemostasis leads to excitotoxicity, which is a major factor for the progression of disorder and contributes to dysregulating the apoptotic schedule of neurons. 12 Apoptosis mainly occurs via the overactivation of the p38 MAPK pathway, which comprises GSK-3β expression, resulting in excitotoxicity and impaired motor coordination. 13 The excitotoxic mechanism in autism has a strong link to the overexpression of the NMDA receptor via inhibition of GSK-3β.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Syringic acid alleviates valproic acid induced autism via activation of p38 mitogen‐activated protein kinase: Possible molecular approach

Mallan

Singh

2023

Environmental Toxicology

View full text Add to dashboard Cite

Autism spectrum disorder (ASD) is a multifactorial neurodevelopmental disorder characterized by restrictive and repetitive behavior followed by impairment in social, verbal, and non‐verbal interaction and communication. Valproic acid (VPA) is a well‐known anti‐epileptic drug, but its prenatal exposure to animals causes social impairment, neurotransmitters imbalance, and neuroinflammation with ASD‐like phenotypes. Syringic acid (SA) is a polyphenolic compound with anti‐inflammatory, anti‐apoptotic, antioxidant, and neuromodulator activity. The purpose of study was to investigate the protective effect of Syringic acid (SA) in prenatal VPA‐treated rats through behavioral, neuroinflammation, oxidative stress, neurotransmitters, neuronal integrity, and apoptotic marker. Single dose of VPA was administered 600 mg/kg, i.p. on a gestational day (GD) 12th and SA was administrated from PnD 26th to 54th at the dose of 25, 50, and 100 mg/kg, p.o. On PnD 56th behavioral parameters (Pain sensitivity, open field test, narrow beam walks test and social impairment test) were performed and all animals were sacrificed, and brain tissue was isolated for oxidative stress (GSH, CAT, and LPO), neuroinflammation (TNF‐α and IL‐6) and neurotransmitters (GABA and Glutamate), histopathology (H&E, Nissl), immunohistochemistry (p38 MAPK) analysis. Rat treated with SA dose‐dependently prevented behavioral alteration, restored antioxidant enzymes, neurotransmitters level, decreased neuroinflammatory markers, and improved neuronal integrity. Furthermore, immunohistochemistry confirmed the reduced p38 MAPK marker expression by SA in VPA induced autistic behavior.

show abstract

“…Propionic acid (PPA) is widely used to model mitochondrial dysfunction in neurons and has been shown to disrupt mitochondrial membrane potential, biogenesis, and respiration in vitro [31][32][33][34][35]. PPA is known to impair neuronal metabolism and alter behavior in vivo and is an established animal model to study neurodevelopmental mechanisms involved in autism spectrum disorder (ASD) [36][37][38][39][40][41][42][43][44]. However, the effect of PPA on mitochondrial morphology and dynamics remains understudied.…”

Section: Introductionmentioning

confidence: 99%

Transmission Electron Microscopy Reveals Propionic Acid-induced Alterations to Mitochondrial Morphology in SH-SY5Y Cells

Buchanan¹,

Mahony²,

Bam³

et al. 2022

Preprint

View full text Add to dashboard Cite

Mitochondrial dysregulation is implicated in numerous neurological disorders. Mitochondrial dynamics, including biogenesis, fusion and fission, are essential components of mitostasis which is modulated by complex regulatory mechanisms. Although expression studies are often used to investigate mitochondrial dynamics, these studies may be limited by the interdependent and temporal nature of mitostasis. Transmission electron microscopy (TEM) and cryogenic preparation methods provide a direct approach to examine mitochondrial ultrastructure in neurons. We investigated the utility of TEM to visualize mitochondrial morphological changes in SH-SY5Y cells treated with propionic acid (PPA). We examined whether morphological alterations were associated with differences in membrane potential or expression of biogenesis, fusion and fission genes. PPA induced a significant decrease in mitochondrial area (p<0.01 5mM), Feret's diameter and perimeter (p<0.05 5mM), and in area2 (p<0.05 3mM, p<0.01 5mM) – consistent with a shift towards fission. Morphological changes were not associated with significant differences in mitochondrial membrane potential. However, we observed decreased gene expression of NRF1 (p<0.01), TFAM (p<0.05), and STOML2 (p<0.0001). These data support a disruption of the balance in dynamics to preserve function under stress. This demonstrates the utility of TEM to provide insight into mitochondrial dynamics and function which can inform targeted mechanistic investigations into neuropathology.

show abstract

Protective Effects of Bee Pollen on Multiple Propionic Acid-Induced Biochemical Autistic Features in a Rat Model

Cited by 8 publications

References 58 publications

Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World

Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World

Syringic acid alleviates valproic acid induced autism via activation of p38 mitogen‐activated protein kinase: Possible molecular approach

Transmission Electron Microscopy Reveals Propionic Acid-induced Alterations to Mitochondrial Morphology in SH-SY5Y Cells

Contact Info

Product

Resources

About