Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress

Xu, Mulin; Wei, Quanwei; Zheng, Kaizhi; Mao, Dagan; Zheng, Yutu; Li, Yan

doi:10.1016/j.acthis.2014.08.003

Cited by 11 publications

(8 citation statements)

References 46 publications

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“…It is known that corticosterone levels are elevated under stress induced by excess ROS, leading to increased MDA levels and tissue injury (Bhatia et al, 2011;Prabsattroo et al, 2015;Arun et al, 2016aArun et al, , 2016b. Some natural products and plants have been developed and used as alternative medicines to diminish stress by reducing the levels of cortisol and oxidants (Bitgul et al, 2013;Xu et al, 2014;Prabsattroo et al, 2015;Lee et al, 2017). Consistent with previous reports, we found that PE leaf extract significantly reduced corticosterone and testicular MDA levels in rats subjected to restraint (Figs.…”

Section: Discussionsupporting

confidence: 92%

Phyllanthus emblica leaf extract ameliorates testicular damage in rats with chronic stress

Arun

Burawat

Yannasithinon

et al. 2018

J. Zhejiang Univ. Sci. B

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Stress affects the male reproductive system and can cause sub-fertility or infertility. Although Phyllanthus emblica L. (PE) extract has been shown to have high antioxidant capacity and protective properties in damaged tissue, the preventive effects of PE extract on testicular function from stress-related impairment have never been demonstrated. This study aimed to investigate the effects of PE aqueous leaf extract on testicular impairment and protein marker changes in rats suffering from chronic stress. Adult male rats were divided into four groups: a control group, a chronic stress (CS) group, and two groups with CS that received different doses of PE extract (50 or 100 mg/kg body weight (BW)). In the treatment groups, the animals were given PE extract daily before stress induction for 42 consecutive days. Stress was induced through immobilization (4 h/d) followed by forced cold swimming (15 min/d). Sperm quality and the histology of the testes and caudal epididymis were examined, as were levels of serum corticosterone, testosterone, and malondialdehyde (MDA). The expressions of testicular steroidogenic acute regulatory (StAR) and tyrosine-phosphorylated proteins were investigated using immuno-Western blot analysis, as these proteins are assumed to play important roles in spermatogenesis and androgen synthesis. The results showed that PE (50 mg/kg BW) significantly increased sperm concentration and testosterone levels, while decreasing corticosterone levels, MDA levels, sperm head abnormalities, and acrosome-reacted sperm in CS rats. In addition, PE at both doses was found to diminish testicular histopathology in the CS rats. We also found that 50 mg/kg BW of PE significantly improved StAR protein expression and altered the intensities of some tyrosine-phosphorylated proteins in testis. We conclude that PE leaf extract at 50 mg/kg BW can prevent testicular damage in rats with CS.

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Section: Discussionsupporting

confidence: 92%

Phyllanthus emblica leaf extract ameliorates testicular damage in rats with chronic stress

Arun

Burawat

Yannasithinon

et al. 2018

J. Zhejiang Univ. Sci. B

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“…To additionally clarify the decreased testosterone levels, the disturbances of other steroidogenic machineries such as cytochrome P-450 cholesterol side-chain cleavage enzymes, * P<0.05, compared with the control group scavenger receptor class B, and hydroxysteroid dehydrogenases need to be further investigated. In various studies, the increase of malondialdehyde levels and the decrease of enzymatic activities of catalase, glutathione, and superoxide dismutase have been demonstrated in stress testicular tissue (Zayachkivska et al, 2006;Brzozowski et al, 2008;Bhatia et al, 2011;Warzecha et al, 2011;Kwiecien et al, 2012;Hari Priya and Sreenivasula Reddy, 2012;Xu et al, 2014). The alteration of oxidative stress markers might affect normal spermatogenesis, resulting in decreased sperm concentration (Table 1 and Fig.…”

Section: Discussionmentioning

confidence: 99%

Changes of testicular phosphorylated proteins in response to restraint stress in male rats

Arun

Burawat

Sukhorum

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats. Methods: Male Sprague-Dawley rats were divided into two groups (non-immobilized control and restraint-immobilized/stress groups, n=8 each group). The stress animals were immobilized (12 h/d) by a restraint cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared between groups. In addition, the expression of steroidogenic acute regulatory (StAR) and phosphotyrosine proteins (previously localized in Sertoli and late spermatid cells) in testicular lysate was assayed by immuno-Western blotting. Results: Testosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased while the corticosterone level was increased as compared with the control (P<0.05). Histologically, stress rats showed low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular StAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a testicular 95-kDa phosphorylated protein was significantly decreased in stress rats. Conclusions: This study has demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive parameters in stress rats. It could be an explanation of some infertility in stress males.

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“…Xie et al (12) also demonstrated that exercise protects endothelium continuity through increasing NO production in collateral-dependent porcine coronary arterioles. Furthermore, NO synthesis depends on physical stimuli that modulate the activity of NO synthase (NOS) (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus

Wang

Zhang

et al. 2019

Exp Ther Med

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Previous studies by our group have indicated that exercise intervention can ameliorate endothelial dysfunction, which is an early pathophysiological change of prediabetes mellitus. The present study aimed to test the hypothesis that nitric oxide synthases (NOSs), which are expressed in blood vessel endothelium, contribute to the mitigation of vascular endothelium-dependent dysfunction by aerobic exercise in prediabetes mellitus. A prediabetic rat model was established by feeding the rats an additional high-energy diet, and was confirmed by testing blood glucose levels, the area-under-the-curve for the blood glucose tests (P<0.05) and the changes to the histological morphology of the thoracic aorta. Further examination identified that NOS expression changed significantly between the control and prediabetes groups, indicating endothelial dysfunction in the prediabetic rats. Following aerobic exercise, a significant increase in NOS, endothelial (eNOS) mRNA and protein expression (P<0.05), and a significant decrease in NOS, inducible (iNOS) mRNA and protein expression (P<0.05) was identified in the prediabetic rats compared with the control group. No significant change in nitric oxide synthase, brain expression was observed in the prediabetic rat group compared with the control group. Notably, there was also a significant increase and decrease in eNOS and iNOS activity, respectively, in the prediabetes group compared with the control group (P<0.05). Furthermore, nitric oxide (NO) concentration in the vascular endothelium was detected, which revealed a significant increase in NO concentration in the prediabetic rats following aerobic exercise compared with the control (P<0.05). The present study provided results that demonstrated that aerobic exercise ameliorated the vascular endothelium-dependent dysfunction through the NOS/NO signaling pathway, which is primarily regulated by NOS expression and activity, in prediabetes mellitus. The current study provided the theoretical basis for the use of exercise intervention to prevent diabetes mellitus during the early stage.

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Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress

Cited by 11 publications

References 46 publications

Phyllanthus emblica leaf extract ameliorates testicular damage in rats with chronic stress

Phyllanthus emblica leaf extract ameliorates testicular damage in rats with chronic stress

Changes of testicular phosphorylated proteins in response to restraint stress in male rats

Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus

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