Protective effects of bioactive peptides in foxtail millet protein hydrolysates against experimental colitis in mice

Zhang, Bowei; Xu, Yinbo; Zhao, Chenchen; Zhang, Yunhui; Lv, Huan; Ji, Xiaofu; Wang, Jin; Pang, Wenwen; Wang, Xiaowen; Wang, Shuo

doi:10.1039/d1fo02482e

Cited by 27 publications

(22 citation statements)

References 30 publications

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“…These results are in line with the changes observed in the cell culture model employed in this report, and increase the available evidence about how these food-derived peptides might exert a positive effect on intestinal inflammation. Similarly, Zhang et al 20 reported that protein hydrolysates obtained from foxtail millet could inhibit inflammasome activation and IL-1β expression through the NLRP3/ASC/caspase-1 pathway, both in animal studies and in CACO-2 cells, as tested in this research.…”

Section: Resultsmentioning

confidence: 55%

“…Therefore, as in the previous case, treatment with the two hydrolysates, at both concentrations, showed an anti-inflammatory effect by significantly reducing the expression of IL-6 induced by LPS. In this regard, Zhang et al 20 evaluated whether the oral administration of foxtail millet protein hydrolysates could improve the colon symptoms of dextran sulfate sodium-induced colitis mice. Among other parameters analysed, the authors reported a reduction in the levels of TNF-α and IL-6, in line with the results obtained for the kiwicha peptides employed in this study.…”

Section: Paper Food and Functionmentioning

confidence: 99%

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Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway

Martínez‐López

Rivero‐Pino

Villanueva³

et al. 2022

Food Funct.

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Section: Resultsmentioning

confidence: 55%

Section: Paper Food and Functionmentioning

confidence: 99%

Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway

Martínez‐López

Rivero‐Pino

Villanueva³

et al. 2022

Food Funct.

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“…In the protein-expression level, the dosage of GP-1 and GP-2 all had higher Occludin than the PC group. As revealed in the Caco-2 cells as well as the colitis mice, the treatment of bioactive peptides in foxtail millet protein hydrolysate (FMPH) increased intestinal ZO-1 and Occludin expressions compared with the DSS-induced groups [ 34 ]. In addition, the FMPH was involved in the NF-κB signal by inhibiting its phosphorylation process and then reduced the secretion of TNF-α and IL-6.…”

Section: Discussionmentioning

confidence: 99%

The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice

Xing

Cao

et al. 2022

Nutrients

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The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension. In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice. The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells. In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues. In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment. In the analysis of gut microbiota, the GP was checked to modulate the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria. The above results imply that GP could attenuate DSS-induced colitis by suppressing the inflammatory cytokines and regulating the gut microbiota.

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“…Millet-derived bioactive peptides have been demonstrated to have antioxidant and antifungal activities [ 17 ], and could inhibit the secretion of proinflammatory cytokines in RAW264.7 cells in vitro [ 18 ]. In addition, we found that millet protein hydrolysate, which is the active ingredient of foxtail millet, has protective effects against intestinal mucosal damage and has inhibitory effects on inflammatory responses [ 19 ]. These results suggest that millet protein may have protective effects against gastric ulcers, but its activity has not been studied.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Foxtail Millet Protein Hydrolysate on Ethanol and Pyloric Ligation-Induced Gastric Ulcers in Mice

et al. 2022

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Foxtail millet has been traditionally considered to possess gastroprotective effects, but studies evaluating its use as a treatment for gastric ulcers are lacking. Here, we assessed the antiulcer effects of foxtail millet protein hydrolysate (FPH) and explored its mechanism by using blocking agents. In a mouse model of ethanol-induced gastric ulcers, pretreatment with FPH reduced the ulcerative lesion index, downregulated the expression of inflammatory cytokines in the gastric tissue, increased the activity of antioxidant enzymes, and improved the oxidative status. FPH increased constitutive the activity of nitric oxide synthase (cNOS), NO levels, and mucin expression in gastric mucosa, and inhibited the activation of the ET-1/PI3K/Akt pathway. In a mouse model of pyloric ligation-induced gastric ulcers, FPH inhibited gastric acid secretion and decreased the activity of gastric protease. Pretreatment of mice with the sulfhydryl blocker NEM and the NO synthesis inhibitor L-NAME abolished the gastroprotective effect of FPH, but not the KATP channel blocker glibenclamide and the PGE2 synthesis blocker indomethacin. Among the peptides identified in FPH, 10 peptides were predicted to have regulatory effects on the gastric mucosa, and the key sequences were GP and PG. The results confirmed the gastroprotective effect of FPH and revealed that its mechanism was through the regulation of gastric mucosal mucus and NO synthesis. This study supports the health effects of a millet-enriched diet and provides a basis for millet protein as a functional food to improve gastric ulcers and its related oxidative stress.

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Protective effects of bioactive peptides in foxtail millet protein hydrolysates against experimental colitis in mice

Abstract: It is of great significance to develop a dietary intervention strategy to prevent inflammatory bowel disease (IBD). A millet-rich diet can ameliorate IBD, but the active ingredients and mechanisms remain...

Cited by 27 publications

References 30 publications

Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway

Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway

The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice

Protective Effect of Foxtail Millet Protein Hydrolysate on Ethanol and Pyloric Ligation-Induced Gastric Ulcers in Mice

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