Protective Effects of Bryonia multiflora Extract on Pancreatic Beta Cells, Liver and Kidney of Streptozotocin-Induced Diabetic Rats: Histopathological and Immunohistochemical Investigations

Abstract

Objective: In this study, the ameliorative potential and antioxidant capacity of treated with different doses of Bryonia multiflora extract (BE) was investigated using histopathological and immunohistochemical changes in pancreatic beta cells, liver and kidney tissues of streptozotocin (STZ)-induced diabetic rats. Material and Methods: A total of forty-two healthy adult Wistar albino male rats were divided randomly into six groups as Control (C); Diabetes mellitus (DM); DM+Akarboz 20 mg/kg; DM+100 mg/kg BE extract (BE1); DM+200 mg/kg BE extract (BE2); DM+400 mg/kg BE extract (BE3). Experimental diabetes was established by a single-dose [45 mg/kg, intra-peritoneal (i.p)] STZ injection. Essential dosages of BE extracts and Akarboz were applied with gastric gavage for 21 day. Blood glucose levels were recorded throughout the all experiment period. Results: Histopathological studies showed that hepatorenal and pancreatic protection by depending on the dose level of BE extracts was further supported by the almost normal histology in DM+ BE extract-treated group as compared to the degenerative changes such as disorder of architectural structure, inflammatory cell infiltration, hydropic degeneration and necrosis in pancreas, liver and kidney tissues of STZ-treated rats. Immunohistochemical investigation revealed that STZ-induced degenerative changes in beta-cells in the pancreas of the diabetic rats has reduced insulin immunoreactivity. On the other hand, insulin immunoreactivity in the β cells of pancreas of BE -treated diabetic rats has significantly increased. Additionally, Glutathione peroxidase 1 (GPx1) immunoreactivity was lower in the tissues of diabetic rats (DM group) compared to the other groups. Conclusion: In conclusion, BE extract has a protective effect on tissue damage probably due to its antioxidant activity and possess the ability to regenerate β-cell in STZ-induced diabetic rats.

Antidiabetic Activity of Pistia strateotes L.Aqueous Extract in Alloxan-induced Diabetic Rats

“…These accord with previous studies. 23,24 However, treatment with the P. stratiotes extract for 3 weeks was able to amelioratethese alterations to near normal status. This implies the potential of P. stratiotes extract to ameliorate hepatic and renal diabetes-associated complications.…”

Chemical compositions and hypoglycemic activities of the protein and mucilage of Casimiroa edulis (Llave & Lex) seeds and fruits

“…Intraperitoneal injection of the animals with STZ was done to induce diabetes (Uyar et al, 2017). Blood glucose level was measured after 72 hours by using glucometer after 1, 2, 3, and 4 week's intervals.…”

“…In addition to hepatic GSH reduction as result of increased ROS production and free radical scavenging activity inhibition agreed with Abd El-Fattah et al (2018) and Ahmed et al (2015), as well as other hepatic metabolic disturbances reduction in albumin and total protein, GGT serum elevation, AST and ALT, and ALP elevation after STZ induction was in accordance with histopathological findings and indicator of liver damage and thus otherwise alterations in the hepatic functions (Ahmed et al, 2015). Wang et al (2016) suggested that both oral anti-diabetic drugs and insulin may lead to insulin resistance besides many [E] Showing nearly normal histological structure in group-treated glibenclamide.…”

“…Casimiroa edulis belongs to family Rutaceae and it is widely distributed in Mexico and Central America (Landaverde et al, 2009). C. edulis is an evergreen tree that reaches up to 18 m tall and it is of economically high importance as it produces an edible fruit known as white Sapote (Ya-Ming et al, 2011), the plant under study previously reported to be used as sedative, hypnotic, anticonvulsant, and antihypertensive and also used for dermatological cases. Alkaloids, coumarins, limonoids, and flavonoids, i.e., zapotin and 3, 5-trimethoxy flavones, were previously reported (Awaad et al, 2011).…”

“…Alkaloids, coumarins, limonoids, and flavonoids, i.e., zapotin and 3, 5-trimethoxy flavones, were previously reported (Awaad et al, 2011). Hyperglycemia is a chronic diabetic disease that led to the oxidative stress which was observed particularly in the liver that has a major and critical role in the regulation of carbohydrate metabolism (Uyar et al, 2017).…”