Protective Effects of Chlorogenic Acid Against Amyloid‐Beta‐Induced Oxidative Stress and Ion Transport Dysfunction in SH‐SY5Y Cells

Abstract

Caloric restriction mimetics mimic the beneficial effects of dietary restriction approaches, such as caloric restriction approaches, without limiting the dietary intake. Chlorogenic acid (CGA) acts as a caloric restriction mimetic; however, its neuroprotective mechanisms remain ambiguous. Therefore, in this study, we aimed to elucidate the neuroprotective effects of CGA on SH‐SY5Y cells treated with amyloid‐beta (Aβ)1‐42. Aβ1‐42 (1.25 μM) induced oxidative stress by significantly increasing the pro‐oxidant levels and decreasing the antioxidant levels in the cells. Additionally, it decreased the Na+/K+‐ATPase and Ca2+‐ATPase ion transporter activities and enhanced the acetylcholinesterase activity. However, CGA (100 μM) reversed these Aβ1‐42‐induced changes in SH‐SY5Y cells. Overall, CGA exerted neuroprotective effects against Aβ1‐42‐induced oxidative stress and impaired ion transporter and acetylcholinesterase activities, serving as a promising therapeutic candidate for neurodegenerative diseases, such as Alzheimer's disease.