Protective effects of D-005, a lipid extract from Acrocomia crispa fruits, against ischemia/reperfusion-induced acute kidney injury in rats

Abstract

BackgroundAcute kidney injury (AKI) induced by renal ischemia/reperfusion (IR) is associated with enhanced production of reactive oxygen species in renal tissues. D-005, a lipid extract obtained from Acrocomia crispa fruit, has previously shown antioxidant effects. The aim of this work was to evaluate the effects of D-005 on renal IR-induced AKI in rats.MethodsRats were randomized into seven groups including a negative control group (vehicle) without AKI and six groups with renal IR-induced AKI as follows: a positive control (vehicle); D-005 treatment at 25, 100, 200, or 400 mg/kg; and dexamethasone at 3 mg/kg. All treatments were orally administered as single doses 1 hour before AKI induction. Biomarkers (serum creatinine, urea, and uric acid concentrations), oxidative variables, and histopathological AKI changes were evaluated in blood and kidney tissues.ResultsAll D-005 doses protected against IR-induced AKI in rats by significantly decreasing biomarkers and histopathological AKI changes as assessed by reduced serum concentrations of creatinine, urea, and uric acid. In addition, all D-005 doses decreased tubular damage, as shown by fewer detached cells and casts in the tubular lumen. D-005 reversed oxidation disturbance markers by decreasing malondialdehyde and sulfhydryl group concentrations in plasma and in kidney homogenates and by increasing kidney catalase activity. Dexamethasone, the reference substance, protected against IR-induced AKI in rats by reducing biochemical and histological variables of renal damage in a similar manner.ConclusionAdministration of single oral doses of D-005 markedly and significantly protected against renal IR-induced AKI, possibly due to its known antioxidant effects.

Benefits of D-005, a lipid extract from Acrocomia crispa fruits, in the prevention of acute kidney njury induced by nephrotoxicity in rats

“…In this context, this study constitutes the first evidence of the nephroprotective efficacy of this mixture of fatty acids obtained from Cuban Acrocomia crispa on aminoglycosides-induced renal damage. Moreover, it was in accordance with previous results in preventing ischemia-reperfusioninduced renal damage 15 , and further studies will clarify the mechanisms by which D-005 exerts its nephroprotective effect in both models of AKI.…”

“…The chosen dose of GSE has shown antioxidant effects in previous studies and it falls within the range of doses previously used in nephrotoxicity models 18 - 20 . D-005, the vehicle, and GSE were administered orally by intragastric gavage in order to guarantee that rats receive the exact dose and according to previous studies 15 , 21 . All treatments were administered once daily for seven days.…”

“…Rats were randomized into 7 groups (10 rats/ group): a negative control (treated with vehicle, Tween 65/H 2 O) and six groups which were administered kanamycin to induce AKI: a positive control group treated with Tween 65/H 2 O, four groups treated with D-005 (25,100,200, and 400 mg/kg) and one group treated with GSE (200 mg/kg). The dosage schedule of D-005 was based on its antioxidant and antiinflammatory previous effects 15 . The chosen dose of GSE has shown antioxidant effects in previous studies and it falls within the range of doses previously used in nephrotoxicity models [18][19][20] .…”

“…Recently, D-005 has shown to prevent pathological changes induced by kidney ischemia/reperfusion in rats, by diminishing serum concentrations of creatinine, uric acid, and urea and lessening tubular histopathological changes in kidney cortex. This was linked to D-005 antioxidant effects, since it decreased the concentrations of malondialdehyde (MDA) and sulfhydryl (SH) groups in plasma and kidney homogenate, as well as it has restored catalase (CAT) enzymatic activity in the kidney 15 .…”

Acrocomia spp.: neglected crop, ballyhooed multipurpose palm or fit for the bioeconomy? A review

“…Nutraceutical studies have explored the use of acrocomia fruits as a complement to high-protein diets and the bioaccessibility of essential minerals contained in the pulp, identifying it as a potential source of Cu, Fe, Mn, and Zn with a high nutritional value (Almeida et al 2020;Gonçalves et al 2020). Furthermore, lipid extracts from A. crispa fruits were shown to have properties protecting against lung and kidney diseases in mice (Mena et al 2019;Oyarzábal-Yera et al 2019). The use of kernel and pulp cakes as animal feed is still under exploration, e.g., with regard to rumen degradability in sheep and inclusion of pulp cake in the diets of broilers and rabbits (Ferreira et al 2019a(Ferreira et al , 2019bNeta et al 2019).…”