Protective effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna subjected to hydrogen peroxide

Untitled

“…Daphnia are known to have myogenic heart responding to a variety of agonists and antagonists, similarly to vertebrate animals and optical examination of this organ after treatment with bioactive agents has been used by some authors (Bekker, Krijgsman, 1951;Villegas-Navarro et al, 2003;Campbell et al, 2004;Bownik et al, 2015).This organ is clearly visible through transparent exoskeleton in a light microscope and it is possible to measure its activity. Video analysis of heart functioning in slow motion proved to be a reliable method for a precise determination of heart rate and measure times of cardiac phases without the necessity of slowing down the heart rate by lowering the ambient temperature (Campbell et al, 2004).…”

“…Video analysis of heart functioning in slow motion proved to be a reliable method for a precise determination of heart rate and measure times of cardiac phases without the necessity of slowing down the heart rate by lowering the ambient temperature (Campbell et al, 2004). Alteration of heart rate in Daphnia was previously documented as a response to environmental conditions or bioactive substances (Navarro et al, 2003;Campbell et al, 2004;Bownik et al, 2014;Bownik and Stępniewska, 2015) however, there is a lack of data on such effects induced by clove oil.…”

Assessment of biopolymer stabilized silver nanoparticle for their ecotoxicity on Ceriodaphnia cornuta and antibiofilm activity

“…Bownik and Stępniewsk [54] reported that Daphnia exposed to 5 mg/L H 2 O 2 + 25 mg/L ECT (ectoine) showed reduced heart rate and thoracic limb movement. The results suggest that SAAgNPs was less toxic than silver nitrate but had induced physiological abnormalities (reduced swimming, heart rate and thoracic limb movement) in C. cornuta.…”

Ectoine as a promising protective agent in humans and animals

“…In our earlier studies (53-55) ectoine showed protective effects in Daphnia magna subjected to high temperature by inhibiting heat shock protein Hsp70, catalase activity, and NO radicals. Moreover, ectoine-treated Daphnia magna were more resistant to toxic disinfectants formaldehyde and hydrogen peroxide than untreated controls, as manifested through significantly lower mortality rates, better swimming activity, and better physiological parameters such as heart rate and thoracic limb activity (56). In practice, it could be used as a stabilising agent during the transport of some edible species sensitive to different forms of stress.…”