Protective Effects of Emodin on Oxidized Fish Oil-Induced Metabolic Disorder and Oxidative Stress through Notch-Nrf2 Crosstalk in the Liver of Teleost Megalobrama amblycephala

Song, Changyou; Li, Hongxia; Tang, Yuanyue; Ge, Xianping; Liu, Bo; Xu, Pao

doi:10.3390/antiox11061179

Cited by 18 publications

(11 citation statements)

References 63 publications

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“…26 Besides, emodin mitigates cisplatin-induced augmentation of ROS and oxidative stress in osteosarcoma MG63 cells through activation of the Nrf2/antioxidant response element signaling pathway. 27 In a previous study by Song et al, 28 data suggested that emodin inhibited oxidized fish oil-induced ROS generation in the liver of teleost Megalobrama amblycephala . Moreover, emodin reduced mitochondrial-dependent apoptosis, repressed mitochondrial ROS generation, and accelerated adenosine triphosphate recovery both in a mouse model of renal ischemia/reperfusion injury and in a cellular model of hypoxia/reoxygenation injury.…”

Section: Discussionmentioning

confidence: 92%

Emodin protects against apoptosis and inflammation by regulating reactive oxygen species-mediated NF-κB signaling in interleukin‐1β-stimulated human nucleus pulposus cells

Zhu

Guo

Zhang³

et al. 2023

Hum Exp Toxicol

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Intervertebral disc degeneration (IDD) is a complex degradative disorder associated with inflammation. Emodin, an anthraquinone derivative, possesses strong anti-inflammatory activity. This study focused on the in vitro therapeutic action of emodin in a cellular model of IDD. Human nucleus pulposus cells (NPCs) were stimulated with interleukin‐1 β (IL-1 β) to induce inflammation. Cell Counting Kit-8 and terminal deoxynucleotidyl transferase dUTP nick end labeling staining assays were performed to evaluate the viability and apoptosis of NPCs, respectively. Caspase-3 activity was measured to indirectly assess cell apoptosis. Western blot analysis was performed to detect protein expression levels. Reverse transcription‐polymerase chain reaction was performed for the detection of relative mRNA levels of tumor necrosis factor- α (TNF- α) and IL-6. Enzyme-linked immunosorbent assay was performed to analyze TNF- α and IL-6 secretion. Our results showed that emodin treatment mitigated IL-1β-induced reduction of cell viability in NPCs. Moreover, the increase in reactive oxygen species (ROS) production, apoptotic rate, and caspase-3 activity in IL-1 β-stimulated NPCs was reduced by emodin treatment. Treatment with emodin also abolished IL-1 β-induced inflammation in NPCs, as indicated by reduced secretion of IL-6 and TNF- α. Besides, the increase in expression levels of phosphorylated p65 and nuclear p65 in IL-1 β-stimulated NPCs was suppressed by emodin treatment. Furthermore, inhibition of nuclear factor kappa B (NF- κB) activation with pyrrolidine dithiocarbamate aggravated the protective effects of emodin. These results suggested that emodin protected NPCs against IL-1 β-induced apoptosis and inflammation via inhibiting ROS-mediated activation of NF- κB.

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Section: Discussionmentioning

confidence: 92%

Emodin protects against apoptosis and inflammation by regulating reactive oxygen species-mediated NF-κB signaling in interleukin‐1β-stimulated human nucleus pulposus cells

Zhu

Guo

Zhang³

et al. 2023

Hum Exp Toxicol

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“…Compared to skin, gill, and muscle tissues, the liver and intestine exhibited a more cumulative response to microplastics, indicating an increase in stress responses with prolonged exposure time. However, with prolonged exposure to negative stimuli, the homeostasis in the fish body may become imbalanced [ 64 ], and the stress-related indicators may cease to increase [ 65 ]. In our study, the values of indicators such as ACP in the liver and AKP in the intestine began to decrease on the fifth day after exposure to microplastics.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Stress Responses of Coreius guichenoti to Microplastics with Different Particle Sizes

Wu,

Qiu,

Lin

et al. 2023

Toxics

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The wild population resources of Coreius guichenoti have sharply declined in recent decades, and any negative factors may have a significant impact on their survival. In this study, the enzymatic stress responses of C. guichenoti to 25 and 48 μm polyethylene fragments were explored for the first time. This was achieved by evaluating the changes in physiological and biochemical indicators of the species in response to the environmental stimuli of microplastics. In this study, we observed an early stress response in the external tissues of C. guichenoti following exposure to microplastics. The TP content in skin and muscle and the MDA content in skin, gill and muscle initially showed a significant increase. The skin, gill, and muscle exhibited greater stress responses to M5 particles, whereas M3 particles caused a greater response in the intestine and especially the liver. After the removal of microplastic exposure, the stress state of the C. guichenoti would be alleviated in a short period, but it could not fully recover to the pre-exposure level. In summary, microplastics pose a significant threat to C. guichenoti. While their negative effects can be alleviated by the removal of microplastics exposure, full recovery does not occur in a short period. Continuous monitoring of microplastics in natural waters and targeted aquatic ecological restoration are essential to ensure the normal growth and reproduction of the wild population of C. guichenoti.

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“…HSP70 protects cells from H 2 O 2 , regulating oxidative stress to enable normal cellular function (Hartl et al, 2011). Organisms activate antioxidant defense systems primarily to eliminate toxic substances such as ROS to overcome cellular damage caused by oxidative stress (Song et al, 2022). Representative antioxidant enzymes operating in this context include superoxide dismutase (SOD) and catalase (CAT).…”

Section: Introductionmentioning

confidence: 99%

Melatonin injection and red light irradiation affect the antioxidant response and cell damage in disk abalone (Haliotis discus hannai) exposed to high water temperatures

Kim,

Park

et al. 2024

J Exp Zool Pt A

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The effects of red light‐emitting diode (LED) light irradiation (630 nm, 0.5 W/m2) and melatonin (10−8 and 10−7 M) on oxidative stress and physiological responses in abalones exposed to high temperatures (28°C) were investigated. Changes in messenger RNA (mRNA) expressions of melatonin receptor (MT‐R), heat shock protein 70 (HSP70), and antioxidant enzymes, as well as alterations in H2O2 levels in the hemolymph, were examined. The results revealed that high‐temperature‐stressed abalones treated with melatonin injections or exposed to red LED light showed a significant increase in MT‐R mRNA expression, while HSP70 mRNA expression decreased. Notably, HSP70 mRNA expression levels in the red LED light‐irradiated group were similar to those in the group injected with 10−8 M melatonin after 24 h exposure. Abalones treated with melatonin at 20°C or irradiated with red LED light exhibited decreased H2O2 levels and reduced antioxidant enzyme mRNA expression compared with those of the control group. However, the high‐temperature environment induced oxidative stress in abalones, leading to increased antioxidant enzyme mRNA expression compared with that under 20°C conditions. Moreover, abalones exposed to high‐temperature stress exhibited hepatopancreatic DNA damage, which was attenuated by melatonin treatment or red LED light irradiation. Hence, red LED light reduces oxidative stress, boosts antioxidant enzymes, and alleviates DNA damage in high‐temperature‐stressed abalones, akin to 10−8 M melatonin treatment. Therefore, considering the practical challenges of continuous melatonin administration to abalones, utilizing red LED light emerges as a practical, effective alternative to protect abalones from oxidative stress compared to 10−8 M melatonin treatment.

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Protective Effects of Emodin on Oxidized Fish Oil-Induced Metabolic Disorder and Oxidative Stress through Notch-Nrf2 Crosstalk in the Liver of Teleost Megalobrama amblycephala

Cited by 18 publications

References 63 publications

Emodin protects against apoptosis and inflammation by regulating reactive oxygen species-mediated NF-κB signaling in interleukin‐1β-stimulated human nucleus pulposus cells

Emodin protects against apoptosis and inflammation by regulating reactive oxygen species-mediated NF-κB signaling in interleukin‐1β-stimulated human nucleus pulposus cells

Enzymatic Stress Responses of Coreius guichenoti to Microplastics with Different Particle Sizes

Melatonin injection and red light irradiation affect the antioxidant response and cell damage in disk abalone (Haliotis discus hannai) exposed to high water temperatures

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