Protective effects of Ficus carica seed oil on ischemia and reperfusion injury in a rat model of acute mesenteric ischemia

Abstract

BACKGROUND:The increase in free oxygen radicals and proinflammatory cytokines in the ischemia-reperfusion injury caused by acute mesenteric ischemia are the key responsibilities of intestinal histopathological alterations. It has been reported that Ficus carica and its various parts contain antioxidant and anti-inflammatory compounds recently. Thus, in the present study, we aimed to investigate how Ficus carica seed oil affects intestinal ischemia-reperfusion injury in a rat model. METHODS:In this study, 50 male Wistar albino rats were randomly divided into five equal groups. Negative control (NC), sham-operated (Sham), ischemia and reperfusion (IR), 3 ml/kg/day Ficus carica seed oil (FC3), 6 ml/kg/day Ficus carica seed oil (FC6). IR, FC3 and FC6 groups underwent ischemia and reperfusion procedure for 45+120 min. Only abdominal midline laparotomy was performed in the Sham group for 165 minutes.RESULTS: Tissue levels of TNFα and IL-1β, which were proinflammatory cytokines, were significantly reduced in the FC6 group than the IR group (p<0.05). In FC3 and FC6 groups, the tissue MPO and MDA enzyme levels were significantly lower than the IR group, but there was a significantly greater decrease in the FC6 group than the FC3 group (p<0.05). SOD and CAT enzymes and reduced glutathione levels of FC3 and FC6 groups were significantly lower than IR group (p<0.05); however, there was no statistically significant difference between the FC3 and FC6 groups. FC3 and FC6 groups were histopathologically graded statistically lower than the IR group, and the FC6 group showed a significant decrease than the FC3 group (p<0.05). CONCLUSION:Oral administration of fig seed oil may reverse biochemical and histopathological findings resulting from ischemia-reperfusion injury in an experimental model of acute mesenteric ischemia in rats, probably because of its antioxidant and anti-inflammatory compounds.

When less is more: Experimental Bishop–Koop technique for reduction in the use of laboratory animals for intestinal pathophysiological studies

“…In addition, SI morphological damage and villus recovery were comparable with several previous results published by our group and others. 15–19,40 In the present study, we observed the greatest damage at 30–60 min post-reperfusion in both the BK-IIRI and OSMA groups, with denuded villi and loss of villi (scores 4 and 5, respectively, on the Park/Chiu score) as the main characteristic lesions. The kinetic study facilitated the recovery of the intestinal mucosa, with edema (Park/Chiu score 1–2) as the predominant lesion at 24 h post-reperfusion and slight intestinal damage at 1 week after IIRI (Figure 5c).…”

“…For example, in the SI, superior mesenteric artery clamping model is currently used to evaluate IIRI, requiring one animal for each sample and study time. [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] The same situation occurs in the field of experimental colitis, where old and recent scientific articles use one animal in each LI sample collection. [30][31][32][33][34][35][36][37] For the reasons mentioned above, it is a challenge to develop models that enable reducing the number of animals involved in these studies.…”

Effect of Ficus carica (fig) seed oil administration on GSH levels, necrosis and cast formation in myoglobinuric acute kidney injury

“…In Orak's study, the effects of fig seed oil on small intestine ischemia-reperfusion injury due to experimental mesenteric artery occlusion were investigated by giving 3 ml/kg and 6 ml/kg doses for 10 days. In this study, a non-dose-dependent increase in tissue CAT and GSH levels was obtained in the groups given fig seed oil in intestinal tissue, and a significant improvement was observed in histopathological parameters, more prominently in the high dose group 41 . In our study, an increase in GSH level was observed similarly in the groups to which we gave fig seed oil, a significant difference was obtained in the high-dose group, moreover, it was observed that the histopathological parameters, which we determined similarly with this study, improved more in the high-dose group.…”

The Effects of Ficus Carica Seed Oil Administration on Renal Tas, Tos, Osi, Pon and Total Thiol Values in Renal Ischemia Reperfusion Damage

“…Scientific literature on Ficus carica seed oil reveals that effective dosage is a minimum of 1 ml/kg and an optimum of 3 ml/kg. Therefore, in our study, a Furthermore, it was determined that this dosage was effective as a result of the applied treatment (10,20).…”