Protective effects of fisetin against myocardial ischemia/reperfusion injury

Abstract

The underlying mechanism of the myocardial protective effect of fisetin was studied in a rat ischemia/reperfusion injury model. Sprague-Dawley rats were randomly assigned to seven groups and pretreated with different solutions by gavage administration. A rat model of cardiac ischemia/reperfusion injury was established. Plasma levels of Von Willebrand factor (vWF) were determined by ELISA, flow cytometry was used to determine the level of cardiomyocyte apoptosis and 2,3,5-triphenyltetrazolium staining was used to determine the size of myocardial infarcts. Hematoxylin and eosin-stained sections of myocardial tissues were examined for pathological changes. Expressions of nuclear factor (NF)-κB and matrix metallopeptidase 9 (MMP-9) were measured by immunohistochemistry. Compared with the model group, rats pretreated with fisetin, quercetin and aspirin showed significant prolongation of clotting time, prothrombin time, thrombin time and activated partial thromboplastin time. Fisetin treatment better maintained the integrity of myocardial fibers and nuclear integrity, reduced the percentage of apoptotic myocardial cells, inhibited expression of NF-κB, decreased the loss of MMP-9 and reduced nuclear translocation of NF-kB. Rats pretreated with fisetin also demonstrated a significant decrease in plasma levels of vWF. In addition, the protective effect of fisetin on myocardial cells was found to be dose dependent.

Anti-osteoporosis Effect of Fisetin against Ovariectomy Induced Osteoporosis in Rats: <i>In silico</i>, <i>in vitro</i> and <i>in vivo</i> Activity

“…Estrogen also boost the production of osteoclastogenic cytokines, which act as pro-resorptive factors via boosting the expression of RANKL in osteoblast lineage cells 17) . Flavonoids are the low molecular weight polyphenolic phytoconstituent commonly synthesized in lot of plants 18,19) . Fisetin (IUPAC name-3,3′ ,4′ ,7-tetrahydroxyflavone) , flavonoid, commonly found in various vegetable and fruits such as grape, cucumber, strawberry, onion and persimmon 20,21) .…”

Integrating Ethnobotany, Phytochemistry, and Pharmacology of Cotinus coggygria and Toxicodendron vernicifluum: What Predictions can be Made for the European Smoketree?

“…A protective effect on ischemic cardiomyocytes was observed when fisetin was tested in a rat ischemia/reperfusion injury model. Fisetin reduced apoptosis in myocardial cells, prolonged coagulation and decreased Von Willebrand factor plasma levels (Long et al, 2020). Given the association of neurodegenerative diseases with alterations in gut microbiota, the effects of fisetin as neuroprotective agent have been studied in a mice model of Parkinson's disease.…”

Cardioprotective effect of Butanol Fraction of Rivea ornata against H2O2 Induced Oxidative Stress in H9c2 Cells: Role of its Phenolic Content and Antioxidant Effect

“…To overcome these challenges, administration of antioxidant rich products or medicinal substance before or after reperfusion phase is may be effective strategy to limit the extent of myocardial damage and therefore protect the heart from effects of oxidative stress [6]. So, various researchers from scientific community have been trying to find the effective antioxidant from natural sources, mainly from medicinal plants to combat detrimental effect of reactive oxygen species in ischemic reperfusion injury [7]. The early reports (clinical and preclinical studies) were mainly focus on antioxidant rich plant extract or its compounds particularly phenolic compounds and flavonoids to overcome this issue [8].…”