Protective effects of folic acid on oxidative damage of rat spleen induced by lead acetate

Li, Ning; Zhao, Yali; Shen, Yue; Cheng, Yi; Qiao, Mingqiang; Luo, Song; Huang, Xianqing

doi:10.1016/j.ecoenv.2021.111917

Cited by 25 publications

(15 citation statements)

References 32 publications

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“…FA preadministration upregulated the expression of various antioxidant response-related molecules, including HO-1, in the ovaries and significantly suppressed oxidative stress and inflammation levels caused by radiation exposure, thus playing a role in alleviating radiation-induced damage. This is consistent with the previously reported effect of FA on spleen injury induced by lead acetate and cardiac dysfunction induced by hyperhomocysteinemia (40,41).…”

Section: Discussionsupporting

confidence: 93%

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice

et al. 2022

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Radiological therapy/examination is the primary source of artificial radiation exposure in humans. While its application has contributed to major advances in disease diagnosis and treatment, ionizing radiation exposure is associated with ovarian damage. The use of natural products, either alone or as an adjunct, has become increasingly common for reducing the side effects of radiological therapy during disease treatment. Herein, we explored the protective effect of folic acid (FA), a widely used B vitamin, against radiation-induced ovarian injury and its mechanism of action. Female mice with normal ovarian function were randomly divided into control, FA, radiation, and radiation + FA groups. The intervention strategy included daily intragastric administration of FA (5 mg/kg) for 3 weeks prior to radiation exposure. Mice in the radiation and radiation + FA groups received a single dose of 5 Gy X-ray irradiation. Changes in the estrous cycle were then recorded, and ovarian tissues were collected. Pathophysiological changes as well as reproductive and endocrine-related indexes were determined via H&E staining, immunohistochemistry, Western blot, and ELISA. The reproductive performance and emotional symptoms of animals were also monitored. Our results indicated that FA intervention effectively alleviated ovarian damage, leading to more regular estrous cycles, lesser impairment of follicular morphology and endocrine status, as well as greater germ cell preservation. Reduced levels of oxidative stress, inflammation, and enhanced DNA repair were associated these changes. FA pre-administration improved the reproductive performance, leading to higher pregnancy rates and greater litter sizes. Further, the anxiety levels of animals were significantly reduced. Our results indicate that FA pre-administration significantly alleviates radiation-induced ovarian damage in rodents, highlighting its potential as a protective strategy against radiation exposure in the female population.

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Section: Discussionsupporting

confidence: 93%

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice

et al. 2022

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“…The liver is the main organ responsible for metabolism and thus reflects the health status of the body, the spleen is the human body’s largest immune organ and is involved in the immune response in body fluids and cells, and the intestinal mucosa is a barrier to the penetration of toxins and substances generated by potential pathogens [ 82 , 83 ]. We found that the morphology of liver, spleen, kidney, and colon tissues of mice treated with different doses of DSM 2950 were normal and free of lesions, suggesting that DSM 2950 was non-toxic and non-pathogenic.…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

et al. 2021

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In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations.

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“…It has no significant side effects in the long-term use of small doses ( Field and Stover, 2018 ). Studies have shown that FA is an effective free radical scavenger, which can inhibit lipid peroxidation and reduce the production of malonic acid (MDA) ( Li et al, 2021 ). FA deficiency can cause an increase in homocysteine and a decrease in methionine, which further increases liver lipid synthesis and induces lipid accumulation in liver cells ( Rizki et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Folic Acid Attenuates High-Fat Diet-Induced Osteoporosis Through the AMPK Signaling Pathway

Zhang

Sun

et al. 2022

Front. Cell Dev. Biol.

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Objective: Obesity caused by a high-fat diet (HFD) will expand adipose tissue and cause chronic low-grade systemic inflammation, leading to osteoporosis. Folic acid (FA) is a water-soluble vitamin that plays an essential role in regulating blood lipids and antioxidants. However, the effects and underlying mechanisms of FA in osteoporosis induced by an HFD remain poorly understood. This study aimed to investigate the effect of FA on bone health by using HFD-induced osteoporosis mice.Materials and Methods: Mice were fed a normal diet, HFD or an HFD supplemented with FA (20 μg/ml in drinking water) for 16 weeks. Throughout the 16 weeks study period, the rats were weighed once every week. GTT, ITT and lipid indexes were detected to evaluate the effects of FA on lipid metabolism in the HFD-fed mice. Morphological and structural changes of the femur and tibial bone were observed using micro-CT, HE staining and bone conversion parameters. The expression of MDA, SOD and inflammatory factors were detected to evaluate the effects of FA on oxidative stress and inflammatory response in the HFD-fed mice. Quantitative real-time PCR and Western blot (WB) were used to investigate the AMPK signaling pathway.Results: After the intervention of FA, the body fat rate of obese mice was reduced, and related metabolic disorders such as insulin resistance, hyperlipidemia, and systemic inflammation were alleviated. In correlation with those modifications, FA attenuated bone loss and improved bone microarchitecture, accompanied the number of osteoclasts and adipocytes decreased. Furthermore, FA promoted the phosphorylation of AMPK, thereby promoting the expression of Carnitine palmitoyltransferase 1 (CPT1), nuclear factor erythroid-2 related factor 2 (Nrf2) and antioxidant enzymes.Conclusion: These findings suggest that FA may modulate lipid metabolism and oxidative stress responses activating the AMPK signaling pathway, thereby alleviating HFD-induced osteoporosis. The results from our study provide experimental evidence to prevent HFD-induced osteoporosis.

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Protective effects of folic acid on oxidative damage of rat spleen induced by lead acetate

Cited by 25 publications

References 32 publications

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

Folic Acid Attenuates High-Fat Diet-Induced Osteoporosis Through the AMPK Signaling Pathway

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