Protective effects of Forsythia suspense extract with antioxidant and anti-inflammatory properties in a model of rotenone induced neurotoxicity

Zhang, Shuai; Shao, Siyuan; Song, Xiu-Yun; Xia, Changfa; Yang, Yanan; Zhang, Pei‐Cheng; Chen, Nai‐Hong

doi:10.1016/j.neuro.2015.09.009

Cited by 56 publications

(22 citation statements)

References 52 publications

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“…Our findings suggest that lncRNA may contribute to PD pathogenesis by regulating the expression of the genes related to immune response and inflammation. Moreover, KEGG pathway analysis showed that the altered lncRNA AC007991.2-201, AC084816.1-207, and HOTAIRM1 were enriched in MAPK signaling pathway, which was implicated in the pathogenesis of PD, including inflammation (Zhang et al, 2016) and cell death (Wang et al, 2012). Moreover, we identified that several DE lncRNAs including HOTAIRM1 were enriched in Jak-STAT signaling pathway, which plays a critical role in PD by promoting neuroinflammation and dopaminergic neuron apoptosis (Qin et al, 2016;Zhu et al, 2017).…”

Section: Discussionmentioning

confidence: 83%

Dysregulated Long Non-coding RNAs in Parkinson’s Disease Contribute to the Apoptosis of Human Neuroblastoma Cells

Fan

Yang

et al. 2019

Front. Neurosci.

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The molecular mechanism underlying Parkinson's disease (PD), an increasingly common neurodegenerative disease, remains unclear. Long non-coding RNA (lncRNA) plays essential roles in gene expression and human diseases. We hypothesize that lncRNAs are involved in neuronal degeneration of PD. Using microarray, we identified 122 differentially expressed (DE) lncRNAs and 48 DE mRNAs between the circulating leukocytes from PD patients and healthy controls. There were 714 significant correlations (r ≥ 0.8 or ≤−0.8, p < 0.05) among the DE lncRNAs and mRNAs. Gene function and pathway analysis of the 48 DE mRNAs revealed biological pathways related to PD pathogenesis, including immune response, inflammatory response, MAPK, and Jak-STAT pathway. In a cohort of 72 PD patients and 22 healthy controls, the upregulation of four lncRNAs (AC131056.3-001, HOTAIRM1, lnc-MOK-6:1, and RF01976.1-201) in circulating leukocytes of PD patients were further confirmed. These lncRNAs were also upregulated in THP-1 cells, a human monocytic cell line, after inflammatory stimulation. Interestingly, the conditioned culture medium of THP-1 cells or 6-OHDA significantly increased the expression of these lncRNAs in SH-SY5Y cells, a human neuroblastoma cell line expressing dopaminergic markers. Importantly, overexpression of AC131056.3-001 or HOTAIRM1 increased baseline and 6-OHDAinduced apoptosis of SH-SY5Y cells. Taken together, we identified distinct expression profiles of lncRNA and mRNA in circulating leukocytes between PD patients and healthy controls. Dysregulated lncRNAs such as HOTAIRM1 and AC131056.3-001 may contribute to PD pathogenesis by promoting the apoptosis of dopaminergic neuron.

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Section: Discussionmentioning

confidence: 83%

Dysregulated Long Non-coding RNAs in Parkinson’s Disease Contribute to the Apoptosis of Human Neuroblastoma Cells

Fan

Yang

et al. 2019

Front. Neurosci.

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“…Dopamine and its metabolites, DOPAC, were assayed according to the protocol described by Zhang et al (2016a). Briefly, the striatum of mice was removed after 4 weeks CRS, then weighed and homogenized (1 mg:10 μL) in ice cold 0.6 M HClO 4 solution including internal standard (isoprenaline, 250 ng·mL À1 ).…”

Section: Determination Of Dopamine and Dopacmentioning

confidence: 99%

RTP801 is a critical factor in the neurodegeneration process of A53T α‐synuclein in a mouse model of Parkinson's disease under chronic restraint stress

Zhang

Chu

Wang

et al. 2018

British J Pharmacology

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“…Various signaling pathways are associated with neuroprotective effects, including NF-κB, p38 MAPK and AKT (24,25). AKT exhibits a key role in strengthening cell survival through the inhibition of caspase-activated apoptosis (26).…”

Section: Discussionmentioning

confidence: 99%

Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model

Sun

et al. 2017

Mol Med Report

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The purpose of the present study was to examine the effects of myricetin on reducing cerebral ischemia injury in a rat model. A rat model of permanent middle cerebral artery occlusion (pMCAO) was used in the present study. Rats were randomized into the following five groups: Sham, model, low‑myricetin (1 mg/kg), medium‑myricetin (5 mg/kg) and high‑myricetin (25 mg/kg) groups. Neurological deficit scores were evaluated by an examiner blinded to the experimental groups. Brain infarct size was estimated macroscopically using 2,3,5‑triphenyltetrazolium chloride staining. The levels of inflammatory factors tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑1β, and oxidative stress index superoxide dismutase (SOD), malondiadehyde (MDA), and the glutathione/glutathione disulfide (GSH/GSSG) ratio were measured by ELISA. The degree of brain cell apoptosis was determined using a terminal deoxynucleotidyl transferase dUTP nick‑end labeling assay. Protein expression levels of total or phosphorylated p38 mitogen activated protein kinase (MAPK), nuclear factor (NF)‑κB/p65 and protein kinase B (AKT) were determined using a western blotting assay. The neurological deficit score and infarct area induced by pMCAO decreased in a dose‑dependent manner following myricetin treatment. Furthermore, myricetin reduced the expression levels of IL‑1β, IL‑6, TNF‑α, and MDA, and increased GSH/GSSG ratio and SOD activity. A significant decrease in cell apoptosis was observed in response to myricetin. In addition, myricetin significantly increased the level of phosphorylated AKT protein, and decreased the phosphorylation of p38 MAPK and the level of NF‑κB/p65. Overall, the results of the present study suggested that myricetin exhibits a therapeutic effect by reducing ischemic cerebral injury, and the protective effect of myricetin may be associated with the p38 MAPK, NF‑κB/p65 and AKT signaling pathways.

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Protective effects of Forsythia suspense extract with antioxidant and anti-inflammatory properties in a model of rotenone induced neurotoxicity

Cited by 56 publications

References 52 publications

Dysregulated Long Non-coding RNAs in Parkinson’s Disease Contribute to the Apoptosis of Human Neuroblastoma Cells

Dysregulated Long Non-coding RNAs in Parkinson’s Disease Contribute to the Apoptosis of Human Neuroblastoma Cells

RTP801 is a critical factor in the neurodegeneration process of A53T α‐synuclein in a mouse model of Parkinson's disease under chronic restraint stress

Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model

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