Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation

Abstract

The present study was conducted to determine the effects of adding fruit-juices to semen extenders on viability of buck spermatozoa during cryopreservation in two studies. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris-egg yolk based extenders. In study I, the diluted semen samples were supplemented with orange (Citrus sinensis), cucumber (Cucumis sativus) and pineapple (Ananas comosus) juices (2.5, 5, 7.5 and 10 ml/100 ml). In study II, the diluted semen samples were supplemented with 5 ml/100 ml of different combinations of fruitjuices consisting of pineapple and orange juices (TEYPO) at a 1:1 ratio, pineapple and cucumber juices (TEYPC) at a 1:1 ratio, orange and cucumber juices (TEYOC) at a 1:1 ratio, and pineapple, orange and cucumber juices (TEYPOC) at a 1:1:1 ratio, respectively. The diluted semen samples were cryopreserved and thereafter evaluated for sperm viability parameters. The extenders supplemented with 7.5 and 10% orange, and 10% pineapple had higher (P < 0.05) motility compared to the control. There was higher (P < 0.05) acrosome integrity in extenders supplemented with orange and pineapple at all levels compared to the control while cucumber at 10% had higher (P < 0.05) acrosome integrity compared to other levels and the control. The extenders supplemented with cucumber, orange and pineapple had higher (P < 0.05) membrane integrity compared to the control except at 7.5% of extender supplemented with cucumber. Similarly, the extenders supplemented with cucumber, orange and pineapple had lower (P < 0.05) sperm abnormality compared to the control. The extenders supplemented with cucumber, orange and pineapple at 2.5, 10 and 7.5% respectively had lower (P < 0.05) concentrations of MDA compared to other levels and the control. There was higher motility and acrosome integrity in extenders supplemented with different combinations of fruit-juices (P < 0.05) compared to the control. TEYPC had higher (P < 0.05) membrane integrity and lower (P < 0.05) concentrations of MDA compared to the control. The findings revealed that extenders supplemented with orange and pineapple at 10% consistently improved motility, acrosome integrity and membrane integrity, and reduced sperm abnormality compared to the control while no particular combination of these fruit-juices was consistently superior in all the parameters.

Calidad y fertilidad del semen de conejo diluido con jugo de sandía.

“…Similarly, Tvrda et al (2017) suggest that lycopene supplemented to the semen extender acts as an effective motion-promoting and membrane protecting molecule, significantly improving sperm motility, membrane integrity, and mitochondrial activity during preservation. In agreement with this study is the report of Daramola et al (2016) that supplementing extenders with fruit juice (orange and pineapple) at 10% consistently improved motility, acrosome integrity and membrane integrity, and reduced sperm abnormality. The improvement in semen quality could be attributed to the presence of substances in these fruits such as vitamins and phenolic compounds are known to function as antioxidants (Kiwon et al 2003;Cutler et al, 2008).…”

“…The improvement in semen quality could be attributed to the presence of substances in these fruits such as vitamins and phenolic compounds are known to function as antioxidants (Kiwon et al 2003;Cutler et al, 2008). Gardner et al (2000) had earlier reported that concentrations of vitamin and total phenolic contents in fruit-juices have a strong relationship with antioxidant capacity (Daramola et al 2016). Similarly, Amedu and Idoko (2016) reported that Citrullus lanatus promotes normal sperm morphology, sperm concentration, sperm volume and sperm motility in rat semen, probably due to the antioxidant activity of lycopene present, similar reports also corroborates this claims in chilled or frozen rabbit, turkey and fowl semen (Rosato et al 2012).…”

Effects of apple and orange juices on quality of refrigerated goat semen

“…Vitamins A, C and E have been shown to be potent antioxidants (Breininger et al, 2005;Ondei et al, 2009). The inclusion of fruit juices from orange (Citrus sinensis), cucumber (Cucumis sativus) and pineapple (Ananas comosus) as constituents of semen extenders has recently been proved to improve the quality of stored spermatozoa (Daramola et al, 2016). Apple (Malus domestica) and orange (Citrus sinensis) are antioxidantrich fruits with high levels of these vitamins (Cutler et al, 2008) that probably outweigh other food nutrients in reducing the effect damage by reactive oxygen species.…”

Effect of semen cryopreservation with cucumber (Cucumis sativus) fruit juice (CJ) fortified- Extender on milt quality, viability and oxidative enzyme activity of Clarias gariepinus (Burchell, 1822)

“…Van Vuren and Steyn (2017) [25] noted however that the level, type, concentration, temperature, and exposure period of the fruit juice function to evaluate the quality and viability of the milt. Fruit antioxidants include carotenoids, vitamins, phenolic compounds and flavonoids and have proved to function as singlet and triplet oxygen quenchers, free radical scavengers and peroxide decomposers (Anghel et al, 2010;Daramola et al, 2016;) [6,9,10] . Preservation of fish milt has become an uphill task due to the fact that most artificial cryo-protectants in use have oxidative impact on fish milt spermatozoa.…”