Protective Effects of Fucoidan against Hydrogen Peroxide-Induced Oxidative Damage in Porcine Intestinal Epithelial Cells

Abstract

This study was conducted to evaluate the effectiveness of fucoidan in ameliorating hydrogen peroxide (H2O2)-induced oxidative stress to porcine intestinal epithelial cell line (IPEC-1). The cell viability test was initially performed to screen out appropriate concentrations of H2O2 and fucoidan. After that, cells were exposed to H2O2 in the presence or absence of pre-incubation with fucoidan. Hydrogen peroxide increased the apoptotic and necrotic rate, boosted reactive oxygen species (ROS) generation, and disturbed the transcriptional expression of genes associated with antioxidant defense and apoptosis in IPEC-1 cells. Pre-incubation with fucoidan inhibited the increases in necrosis and ROS accumulation induced by H2O2. Consistently, in the H2O2-treated IPEC-1 cells, fucoidan normalized the content of reduced glutathione as well as the mRNA abundance of NAD(P)H quinone dehydrogenase 1 and superoxide dismutase 1 while it prevented the overproduction of malondialdehyde. Moreover, H2O2 stimulated the translocation of nuclear factor-erythroid 2-related factor-2 to the nucleus of IPEC-1 cells, but this increase was further promoted by fucoidan pre-treatment. The results suggest that fucoidan is effective in protecting IPEC-1 cells against oxidative damage induced by H2O2, which may help in developing appropriate strategies for maintaining the intestinal health of young piglets.

Cellular antioxidant and emulsifying activities of fucoidan extracted from Nizamuddinia zanardinii using different green extraction methods

“…These findings suggest that all the polysaccharide samples are able to inhibit intracellular H 2 O 2 ‐induced ROS production and between fucoidans, those extracted with UM showed the highest ROS scavenging activity. Similar to our results, Li et al (2019) reported that pre‐incubation with commercial fucoidan (extracted from Undaria pinnatifida , Sigma‐Aldrich, St. Louis, MO, USA) inhibited the ROS accumulation in H 2 O 2 ‐treated IPEC‐1 cells. In another study, fucoidan extracted from Ecklonia cava significantly decreased the intracellular ROS contents in H 2 O 2 ‐induced PC‐12 and MC‐IXC cells (Park et al, 2018).…”

Anti-Inflammatory Mechanisms of Fucoidans to Treat Inflammatory Diseases: A Review

“…The results reported by Li et al (2019) and are useful for future studies, as there is limited literature available regarding the effect of fucoidans on NRF2 signal transduction in the anti-inflammatory process [79].…”

“…Furthermore, the authors also described the potential of fucoidans to induce antioxidant proteins, such as HO-1 and SOD, using Western blot analysis [78]. Recently, Li et al (2019) reported that fucoidan treatment can enhance NRF2 translocation from the cytosol to the nucleus as well as NRF2 expression in hydrogen peroxide-induced oxidative damage in porcine intestinal epithelial cells. In addition, reported the NRF2 stimulatory properties of fucoidans in an ICR mouse model and HL-7702 cells.…”

Polysaccharides from brown seaweed: Physicochemical properties, absorption in the intestine, and beneficial effects on intestinal barrier

“…BSP modulates intestinal epithelial cells by preserving cellular antioxidant status and promoting cytokine production. Fucoidan from Undaria pinnatifida protected porcine intestinal epithelial cells IPEC‐1 against hydrogen peroxide (H 2 O 2 )‐induced oxidative damage, which might help develop appropriate strategies for maintaining the intestinal health of young piglets (Y. Li et al., 2019). H 2 O 2 , a source of reactive oxygen species (ROS), rapidly penetrated the cell membrane and generated highly toxic hydroxyl radicals by reacting with intracellular transition metals.…”