Protective Effects of Geraniol on Long Term Renal Ischemia/Reperfusion Injury in Rats

Abstract

Possible protective effects of geraniol, known as antioxidant properties, were analyzed biochemically and histologically on experimental long-term renal ischemia/reperfusion I/R injury in rats. This study used 3-4 months old male Wistar albino rats and were divided into 4 groups (n = 7) by random selection: Group I (Sham Group), Group II (I/R+SF), Group III (I/R+50 mg/kg geraniol), and Group IV (I/R+100 mg/kg geraniol). A right nephrectomy was performed in all groups under anesthesia. Groups I and II were inoculated with SF (1 ml/kg) and Groups III and IV were inoculated with 50 mg/kg and 100 mg/kg of geraniol, injected intraperitoneally. For Groups II, III, and IV, I/R durations were determined to be 60 mins ischemia and 24 hours reperfusion. At the end of the experiment, Urea (BUN), Creatinine (CRE) activities in the blood serum and the catalase (CAT), glutathione peroxidase (GPx) and Superoxide dismutases (SOD), enzyme activities in kidney tissue were measured. Histologic sections were examined by light microscopy using Hematoxylin & Eosine. As a result, it was determined that 100 mg / kg geraniol against renal I/R injury shows more antioxidant effect and protection than 50 mg / kg geraniol. HIGHLIGHTS • Geraniol (100 mg/kg geraniol) protected the kidneys against oxidative stress. • 100 mg / kg geraniol showed antioxidant properties and supported the antioxidant defense system. • SOD, CAT and GPx activities in renal tissue were analyzed through jel electrophoresis. • Geraniol decreased BUN and CRE levels in kidney parameters in rats. 2 Can, S.; et al.

The Protective Effects of Geraniol Against Damage of Short Term Renal Ischemia-Reperfusion in Rats

“…In a similar study we conducted in 2019, the effect of geraniol was evaluated against longerterm I/R (60 min/24h) damage, and geraniol was seen to have increased SOD and CAT enzyme activities. This difference monitored from the angle of SOD and CAT activities between our present study and a study we have made previously is interesting (Can, Canbek, 2019). On the other hand, there are studies showing that antioxidant enzymes decrease as a result of I/R (Singh, Chander, Chopra, 2005).…”

“…We had investigated the protective effect of geraniol on long-term renal ischemia/reperfusion injury in our previous study. As a result of 60 minutes of ischemia and 24 hours of reperfusion, following administration of 50 mg/kg and 100 mg/kg doses of geraniol intraperitoneally; the 100 mg/kg administered dose of geraniol was observed to have a protective effect on the kidney (Can, Canbek, 2019).…”

Antimicrobial Properties of Distichochlamys citrea M.F. Newman Rhizome n‐Hexane Extract against Streptococcus pyogenes: Experimental Evidences and Computational Screening

“…Besides, an acyclic isoprenoid monoterpene contained in the volatile compounds of Distichochlamys citrea extract was geraniol. The pharmacological effects of geraniol suggest that it could be a viable medication candidate for treating a variety of diseases thanks to its anti‐cancer, anti‐inflammatory, antioxidative, antibacterial, hepatoprotective, cardioprotective, and neuroprotective properties [17–19] . Especially, the monoterpenes geraniol and geranyl acetate, volatile bioactive compounds, present in the Distichochlamys citrea and several other plants were proved to exhibit significant anticancer activities against colon cancer cells [20] .…”

“…of diseases thanks to its anti-cancer, anti-inflammatory, antioxidative, antibacterial, hepatoprotective, cardioprotective, and neuroprotective properties. [17][18][19] Especially, the monoterpenes geraniol and geranyl acetate, volatile bioactive compounds, present in the Distichochlamys citrea and several other plants were proved to exhibit significant anticancer activities against colon cancer cells. [20] In these reports, evidences indicated that they are promising for the development of new cancer therapies, thus deserving further investigations.…”