Protective Effects of Ginsenosides (20R)‐Rg3 on H2O2‐Induced Myocardial Cell Injury by Activating Keap‐1/Nrf2/HO‐1 Signaling Pathway

Zhao, Yan; Wang, Yu; Zhang, Min; Gao, Yugang; Yan, Zhaowei

doi:10.1002/cbdv.202001007

Cited by 13 publications

(5 citation statements)

References 45 publications

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“…It is generally believed that excessively ROS levels and the decreased antioxidant enzyme and propylene glycol (MDA) levels indicate the occurrence of oxidative stress. By contrast with the LPS-induced oxidative stress model, an oxidative stress model was created by treating IPEC-J2 cells with 10 mol/mL H 2 O 2 , as previously reported ( 44 , 45 ). In the H 2 O 2 -induced oxidative stress model, baicalin significantly increased the mRNA expression of HO-1 and NQO-1 and significantly increased nuclear protein expression of Nrf2, which revealed that baicalin could alleviate oxidative stress responses in IPEC-J2 cells.…”

Section: Discussionmentioning

confidence: 99%

Baicalin Alleviates LPS-Induced Oxidative Stress via NF-κB and Nrf2–HO1 Signaling Pathways in IPEC-J2 Cells

et al. 2022

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Baicalin is a natural plant extract with anti-inflammatory and anti-oxidant activities. However, the molecular mechanism of baicalin on oxidative stress in IPEC-J2 cells exposed to LPS remains to be unclear. In this study, LPS stimulation significantly increased Toll-like receptor 4, tumor necrosis factor-α, and interleukins (IL-6 and IL-1β) expression in IPEC-J2 cells, and it activated the nuclear factor (NF-κB) expression. While, baicalin exerted anti-inflammatory effects by inhibiting NF-κB signaling pathway. LPS stimulation significantly increased the levels of the oxidative stress marker MDA, inhibited the anti-oxidant enzymes catalase and superoxide dismutase, which were all reversed by baicalin pre-treatment. It was found that baicalin treatment activated the nuclear import of nuclear factor-erythroid 2 related factor 2 (Nrf2) protein, and significantly increased the mRNA and protein expression of its downstream anti-oxidant factors such as heme oxygenase-1 and quinone oxidoreductase-1, which suggested that baicalin exerted anti-oxidant effects by activating the Nrf2-HO1 signaling pathway. Thus, pretreatment with baicalin inhibited LPS - induced oxidative stress and protected the normal physiological function of IPEC-J2 cells via NF-κB and Nrf2–HO1 signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Baicalin Alleviates LPS-Induced Oxidative Stress via NF-κB and Nrf2–HO1 Signaling Pathways in IPEC-J2 Cells

et al. 2022

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“…In addition to this, dysregulated levels of oxidation in the liver further induce liver damage. Among other things, ROS are transmitted to mitochondria via the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system as well as the respiratory chain [9,10]. Apart from mitochondria, overexpression of cytochrome P450 2E1 (CYP2E1) and 4-Hydroxynonenal (4-HNE) further damage hepatocytes [11].…”

Section: Introductionmentioning

confidence: 99%

Alleviation of D-gal-induced senile liver injury by Rg3, a signature component of red ginseng

Ding

et al. 2023

Aging

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To investigate the mechanism by which ginsenoside Rg3 regulates oxidative stress (OS) and inflammation through NF/KB pathway to delay mouse liver injury. This work randomized Balbc mice as four groups: Normal, D-gal, Rg3-L, Rg3-H. Paraffin-embedded liver tissue sections were prepared, later, BAX/BCL-2 protein expression was observed by HE, Sirius red, TUNEL and immunofluorescence to detect apoptotic injury and α-SMA/TGF-β protein expression to detect fibrosis, and liver inflammation-related protein NF-KB was detected. HE and TUNEL staining showed that Rg3 reduced necrotic cells and fibrosis in liver-injured mice, Rg3 increased anti-inflammatory cytokine IL-18 and reduced TNF-α, IL-1β and IL-6 expression. Conclusion: Ginsenoside Rg3 can effectively antagonize D-gal's role in mouse liver injury, and its mechanism may be associated with regulating inflammatory pathway by Rg.

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“…The cells were then washed with PBS three times to remove the DCFH-DA which did not enter the cells. Cellular ROS content assessment was performed using a fluorescent microscope (Olympus, Tokyo, Japan) [67].…”

Section: Evaluation Of Oxidative Stressmentioning

confidence: 99%

Palmatine Protects PC12 Cells and Mice from Aβ25-35-Induced Oxidative Stress and Neuroinflammation via the Nrf2/HO-1 Pathway

Wang,

Pei,

Chen

et al. 2023

Molecules

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Alzheimer’s disease is a common degenerative disease which has a great impact on people’s daily lives, but there is still a certain market gap in the drug research about it. Palmatine, one of the main components of Huangteng, the rattan stem of Fibraurea recisa Pierre (Menispermaceae), has potential in the treatment of Alzheimer’s disease. The aim of this study was to evaluate the neuroprotective effect of palmatine on amyloid beta protein 25–35-induced rat pheochromocytoma cells and AD mice and to investigate its mechanism of action. CCK8 assays, ELISA, the Morris water maze assay, fluorescent probes, calcein/PI staining, immunofluorescent staining and Western blot analysis were used. The experimental results show that palmatine can increase the survival rate of Aβ25-35-induced PC12 cells and mouse hippocampal neurons, reduce apoptosis, reduce the content of TNF-α, IL-1β, IL-6, GSH, SOD, MDA and ROS, improve the learning and memory ability of AD mice, inhibit the expression of Keap-1 and Bax, and promote the expression of Nrf2, HO-1 and Bcl-2. We conclude that palmatine can ameliorate oxidative stress and neuroinflammation produced by Aβ25-35-induced PC12 cells and mice by modulating the Nrf2/HO-1 pathway. In conclusion, our results suggest that palmatine may have a potential therapeutic effect on AD and could be further investigated as a promising therapeutic agent for AD. It provides a theoretical basis for the development of related drugs.

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Protective Effects of Ginsenosides (20R)‐Rg3 on H₂O₂‐Induced Myocardial Cell Injury by Activating Keap‐1/Nrf2/HO‐1 Signaling Pathway

Cited by 13 publications

References 45 publications

Baicalin Alleviates LPS-Induced Oxidative Stress via NF-κB and Nrf2–HO1 Signaling Pathways in IPEC-J2 Cells

Baicalin Alleviates LPS-Induced Oxidative Stress via NF-κB and Nrf2–HO1 Signaling Pathways in IPEC-J2 Cells

Alleviation of D-gal-induced senile liver injury by Rg3, a signature component of red ginseng

Palmatine Protects PC12 Cells and Mice from Aβ25-35-Induced Oxidative Stress and Neuroinflammation via the Nrf2/HO-1 Pathway

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