Protective effects of glutamine preconditioning on ischemia-reperfusion injury in rats

Zhang, Wanxing; Zhou, Lifang; Zhang, Lei; Bao, Lei; Wang, Chuncheng; Meng, Hao; Yin, Wen

doi:10.1016/s1499-3872(11)60011-8

Cited by 18 publications

(16 citation statements)

References 26 publications

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“…This is evidenced by the improvement in hepatic and intestinal morphology, increases in serum TP and ALB levels, reduction in liver enzymes (markers of hepatocyte damage) and decreases in oxidative and inflammatory stress and apoptosis. The protective effects of Gln against hepatic I/R in rats are consistent with those found in previous studies [17,18]. Zhang et al [17] reported that Gln preconditioning effectively protected against hepatic I/R injury.…”

Section: Discussionsupporting

confidence: 89%

“…The protective effects of Gln against hepatic I/R in rats are consistent with those found in previous studies [17,18]. Zhang et al [17] reported that Gln preconditioning effectively protected against hepatic I/R injury. These protective effects were related to the dose of Gln and due to the reduction in intracellular calcium overload and improvements in the activity of Na + /K + ATPase and SOD.…”

Section: Discussionsupporting

confidence: 89%

“…For rodents, some reports have demonstrated that pretreatment with Gln helps to reduce I/R injury in a variety of organs by using different I/R animal models [11,17,18,19,20]. Nevertheless, few studies have been carried out on this topic using the obstructive jaundice hepatic I/R model.…”

Section: Introductionmentioning

confidence: 99%

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Preconditioning with Glutamine Protects against Ischemia/Reperfusion-Induced Hepatic Injury in Rats with Obstructive Jaundice

Dai

Peng

et al. 2014

Pharmacology

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Objective: To ascertain whether glutamine (Gln) pretreatment protects rats with obstructive jaundice from hepatic ischemia/reperfusion (I/R) injury and to determine the underlying molecular mechanisms. Methods: An obstructive jaundice rat model was developed by bile duct ligation. On the first day after the operation, all rats were randomized into two groups and received oral Gln or normal saline (NS) daily for 7 days. Then both groups underwent a 15-min liver ischemia via the Pringle maneuver. Blood samples as well as liver and intestinal tissues were harvested and measured after 1, 6 and 24 h of reperfusion. Results: The results showed that the histological morphology of the liver and intestinal tissues significantly improved in the Gln group after I/R injury compared with the NS group. Serum proteins and enzymes associated with hepatic function also significantly improved in the Gln group. The level of glutathione increased and the levels of malondialdehyde and myeloperoxidase decreased in the Gln group. The levels of interleukin-1β and tumor necrosis factor-α decreased in the Gln group. Moreover, bcl-2 protein expression was upregulated and intercellular adhesion molecule 1 and bax protein expression downregulated in the Gln group; the caspase 3 mRNA level significantly increased in the Gln group. Conclusions: The study demonstrates that preconditioning with Gln significantly improves hepatic structure and function after I/R injury in rats with obstructive jaundice. The protective effect of Gln was mediated by the inhibition of reactive oxygen species and inflammation as well as a reduction in hepatocyte apoptosis.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

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Preconditioning with Glutamine Protects against Ischemia/Reperfusion-Induced Hepatic Injury in Rats with Obstructive Jaundice

Dai

Peng

et al. 2014

Pharmacology

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“…Also, GLN is the precursor of glutathione (GSH), one of the most important antioxidants in the body 11 . An article published recently shows that preconditioning with GLN protects the liver from I/R damage, when subjected to ischemia for one hour followed by reperfusion for 2 hours 12 . Whereas the studies cited, this study was aimed to evaluate the effects of preconditioning with L-Ala-Gln in rat livers subjected to ischemia for 30 minutes followed by reperfusion for 60 minutes, on indicators of liver injury (ALT, LDH) and the expression of Caspase-3.…”

Section: Introductionmentioning

confidence: 99%

Preconditioning with L-alanyl-glutamine reduces hepatic ischemia-reperfusion injury in rats

Júnior¹,

Júnior²,

Vasconcelos³

et al. 2011

Acta Cir. Bras.

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“…The mechanism is still incompletely understood and may be partly related to the preservation of GSH content 15,16 . Another study has demonstrated that GLN preconditioning protects effectively against hepatic ischemiareperfusion injury 17 . Considering the known protective effects of GLN on ischemia/reperfusion, the aim of this study is therefore to evaluate the effect L-Ala-Gln preconditioning on metabolic and histopathological parameters in a gerbil model of acute cerebral ischemia/reperfusion injury.…”

mentioning

confidence: 99%

Preconditioning with L-alanyl-L-glutamine in a Mongolian Gerbil model of acute cerebral ischemia/reperfusion injury

Pires¹,

Souza

Guimarães³

et al. 2011

Acta Cir. Bras.

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PURPOSE:To investigate the effect of L-alanyl-L-glutamine (L-Ala-Gln) preconditioning in an acute cerebral ischemia/reperfusion (I/R) model in gerbils. METHODS: Thirty-six Mongolian gerbils (Meriones unguiculatus), (60-100g), were randomized in 2 groups (n=18) and preconditioned with saline 2.0 ml (Group-S) or 0.75g/Kg of L-Ala-Gln, (Group-G) administered into the femoral vein 30 minutes prior to I/R. Each group was divided into three subgroups (n=6). Anesthetized animals (urethane, 1.5g/Kg, i.p.) were submitted to bilateral occlusion of common carotid arteries during 15 minutes. Samples (brain tissue and arterial blood) were collected at the end of ischemia (T 0 ) and after 30 (T 30 ) and 60 minutes (T 60 ) for glucose, lactate, myeloperoxidase (MPO), thiobarbituric acid reactive substances (TBARS), glutathione (GSH) assays and histopathological evaluation. RESULTS: Glucose and lactate levels were not different in studied groups. However glycemia increased significantly in saline groups at the end of the reperfusion period. TBARS levels were significantly different, comparing treated (Group-G) and control group after 30 minutes of reperfusion (p<0.05) in cerebral tissue. Pretreatment with L-Ala-Gln promoted a significant increase in cerebral GSH contents in Group-G at T30 (p<0.001) time-point compared with Group-S. At T 30 and T 60 , increased levels of GSH occurred in both time-points. There were no group differences regarding MPO levels. Pyknosis, presence of red neurons and intracellular edema were significantly smaller in Group-G. CONCLUSION: Preconditioning with L-Ala-Gln in gerbils submitted to cerebral ischemia/reperfusion reduces oxidative stress and degeneration of the nucleus (pyknosis) and cell death (red neurons) in the cerebral tissue.

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Protective effects of glutamine preconditioning on ischemia-reperfusion injury in rats

Cited by 18 publications

References 26 publications

Preconditioning with Glutamine Protects against Ischemia/Reperfusion-Induced Hepatic Injury in Rats with Obstructive Jaundice

Preconditioning with Glutamine Protects against Ischemia/Reperfusion-Induced Hepatic Injury in Rats with Obstructive Jaundice

Preconditioning with L-alanyl-glutamine reduces hepatic ischemia-reperfusion injury in rats

Preconditioning with L-alanyl-L-glutamine in a Mongolian Gerbil model of acute cerebral ischemia/reperfusion injury

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