Protective effects of gomisin N against hepatic steatosis through AMPK activation

Yun, Ye‐Rang; Kim, Jihyun; Kim, Ji Ha; Jung, Myeong Ho

doi:10.1016/j.bbrc.2016.11.164

Cited by 30 publications

(28 citation statements)

References 14 publications

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“…Our results successfully demonstrate a beneficial effect of GN on reversing the devastating effect of a high-fat diet on the viability of affected animals. Recent reports indicate the pharmacological effect of GN on the prevention of hepatic steatosis in high-fat diet-fed mice [ 10 , 29 ]. Here, we provide another layer of experimental evidence to support the anti-obesity effect of GN by utilizing Drosophila , a well-established in vivo animal model to study molecular mechanisms underlying various human disorders.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated the various pharmacological activities of S. chinensis , including anti-obesity, anti-cancer, anti-inflammatory, anti-oxidant, hepato-protective, and neuroprotective effects [ 6 , 7 , 8 ]. Recently, several reports have shown the involvement of GN in hepatic steatosis and adipocyte differentiation [ 9 , 10 ]. Jang et al revealed that the high-fat diet (HFD)-fed obese mice displayed a higher total body and epididymal adipose tissue weight as well as elevated serum levels of glucose and triglycerides compared to normal diet-fed mice [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…GN was also capable of significantly inhibiting the differentiation of 3T3-L1 preadipocytes at an early adipogenic stage through impairment of mitotic clonal expansion caused by cell cycle arrest at the G1/S phase transition [ 9 ]. Moreover, Yun et al have recently demonstrated GN-dependent activation of the AMPK pathway, resulting in suppressed HFD-induced hepatic steatosis [ 10 ]. Despite recent efforts in deciphering molecular networks underlying the effects of GN on obesity in vitro, functional characterization of GN ultimately requires a systematic investigation in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Functional Characterization of Gomisin N in High-Fat-Induced Drosophila Obesity Models

Lee

Cheon

2020

IJMS

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Gomisin N (GN) is lignin derived from Schisandra chinensis that has been reported to exhibit hepato-protective, anti-cancer, and anti-inflammatory effects. However, its role in whole-body energetic homeostasis remains unclear. In this study, we employed Drosophila melanogaster as a diet-induced obese model to elucidate the effects of GN on lipid and glucose metabolism by measuring climbing activity, triglyceride levels, and lifespan under a rearing condition of a high-fat diet (HFD) containing 20% coconut oil, with or without GN. Constant exposure of flies to an HFD resulted in increased body weight and decreased climbing activity, along with a shortened life span. Importantly, the administration of GN to HFD groups lowered their body weight and induced a specific upregulation of lipid storage droplet (Lsd)-2 and hormone-sensitive lipase (Hsl), in addition to improved lifespan. Importantly, GN in HFD groups appeared to downregulate heat shock protein Hsp90 family member (dGRP94), a key regulator of the endoplasmic reticulum stress response, which may also contribute to improved life span in the presence of GN. Taken together, these in vivo findings suggest that GN could serve as a useful agent for the prevention and treatment of obesity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional Characterization of Gomisin N in High-Fat-Induced Drosophila Obesity Models

Lee

Cheon

2020

IJMS

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“…GN is a major lignan derived from SC, which has been widely used as a traditional herbal medicine in Asian countries. GN possesses known neuroprotective, hepatoprotective and anti-cancer properties [39][40][41]. A recent study suggested that SC suppresses obesity in high-fat diet-induced obese rats and 3T3-L1 cells by decreasing expression of C/EBPα and PPARγ.…”

Section: Discussionmentioning

confidence: 99%

Gomisin N from Schisandra chinensis Ameliorates Lipid Accumulation and Induces a Brown Fat-Like Phenotype through AMP-Activated Protein Kinase in 3T3-L1 Adipocytes

Lee

Kim

et al. 2020

IJMS

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Obesity results from an imbalance between energy intake and energy expenditure, in which excess fat is stored as triglycerides (TGs) in white adipocytes. Recent studies have explored the anti-obesity effects of certain edible phytochemicals, which suppress TG accumulation and stimulate a brown adipocyte-like phenotype in white adipocytes. Gomisin N (GN) is an important bioactive component of Schisandra chinensis, a woody plant endemic to Asia. GN has antioxidant, anti-inflammatory and hepatoprotective effects in vivo and in vitro. However, the anti-obesity effects of GN in lipid metabolism and adipocyte browning have not yet been investigated. In the present study, we aimed to determine whether GN suppresses lipid accumulation and regulates energy metabolism, potentially via AMP-activated protein kinase (AMPK), in 3T3-L1 adipocytes. Our findings demonstrate that GN inhibited adipogenesis and lipogenesis in adipocyte differentiation. Also, GN not only increased the expression of thermogenic factors, including uncoupling protein 1 (UCP1), but also enhanced fatty acid oxidation (FAO) in 3T3-L1 cells. Therefore, GN may have a therapeutic benefit as a promising natural agent to combat obesity.

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“…SREBP-1c and SREBP-2 are the preponderant isoforms and control the levels of ACC and HMGR in hepatic cells, which were the first enzymes recognized as downstream targets for AMPK and key enzymes in FA and TC synthesis [35]. SREBP-1c is a critical transcription factor for increasing the expression levels of lipogenesis genes and plays a significant part in the pathogenesis of hepatic steatosis [36,37]. SREBP-1c can up-regulate the expression level of lipogenic enzymes of ACC, which is responsible for the biosynthesis of malonyl-CoA as the rate-controlling enzyme.…”

Section: Effects Of Viapw (M7) and Irwww (M10) On The Expression Levementioning

confidence: 99%

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Wang

Pan

et al. 2020

Applied Sciences

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In this work, the hypolipidemic activities of two pentapeptides (VIAPW and IRWWW) from miiuy croaker (Miichthys miiuy) muscle on oleic acid (OA)-induced lipid accumulation in HepG2 cells were investigated. VIAPW and IRWWW could significantly inhibit lipid accumulation induced by OA and decreased intracellular levels of intracellular triglyceride (TG) and total cholesterol (TC) in a dose-effect dependence manner. At the concentration of 100 μm, the TG levels of VIAPW (0.201 ± 0.006 mm) and IRWWW (0.186 ± 0.005 mm) were very (p < 0.01) and extremely (p < 0.001) significantly lower than those (0.247 ± 0.004 mm) of the OA model group; the levels of TC of VIAPW (45.88 ± 0.74 μg/mg protein) and IRWWW (41.02 ± 0.14 μg/mg protein) were very (p < 0.01) and extremely (p < 0.001) significantly lower than that (53.45 ± 0.10μg/mg protein) of the OA model group (p < 0.01). The hypolipidemic mechanisms of VIAPW and IRWWW were to down-regulate the expression levels of genes of SREBP-1c, SREBP-2, FAS, ACC, and HMGR in lipid synthesis and to up-regulate the expression levels of genes of PPARα, ACOX-1, and CPT-1 in lipid oxidation. These results suggested that VIAPW and IRWWW could play their hypolipidemic activities in HepG2 cells through regulation of AMPK pathway and act as hypolipidemic nutrient ingredients applied in public healthy and functional foods.

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Protective effects of gomisin N against hepatic steatosis through AMPK activation

Cited by 30 publications

References 14 publications

Functional Characterization of Gomisin N in High-Fat-Induced Drosophila Obesity Models

Functional Characterization of Gomisin N in High-Fat-Induced Drosophila Obesity Models

Gomisin N from Schisandra chinensis Ameliorates Lipid Accumulation and Induces a Brown Fat-Like Phenotype through AMP-Activated Protein Kinase in 3T3-L1 Adipocytes

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

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