Protective effects of green tea polyphenol against cisplatin-induced nephrotoxicity in rats

Abstract

ObjectiveThis study is to compare the effects of green tea polyphenol (GTP) pre-treatment with those of GTP post-treatment on cisplatin (CP)-induced nephrotoxicity in rat.MethodsMale Sprague-Dawley rats were randomly divided into six groups. Animals in the control group received 0.9% saline (intraperitoneal); animals in the GTP group received 0.9% saline and GTP (0.2% GTP as their sole source of drinking water); the CP group received only CP (7 mg/kg, intraperitoneal); the CP+preGTP group received GTP from two days before CP to four days after CP and the CP+postGTP group received GTP for four days after CP. CP-induced renal toxicity was evaluated by plasma creatinine and blood urea nitrogen (BUN) concentrations; kidney tissue γ-glutamyl transpeptidase (GGT) and alkaline phosphatase (AP) activities and histopathological examinations.ResultsHigh serume creatinine and BUN concentrations were observed in CP treated rats. The GGT and AP activites were lower in kidney of CP treated rats compared to control rats. In addition, treatment with CP resulted in development of a marked tubular necrosis, and tubular dilation in kidney of rats. Pretreatment with GTP resulted in markedly reduced elevation of serum creatinine and BUN amounts and changes of GGT and AP activity in kidney induced by CP. CP-induced histopathological changes, including tubular necrosis and dilation, were ameliorated in GTP pre-treated rats, compared to CP alone or GTP post-treated rats.ConclusionThese results demonstrate that GTP might have some protective effect against CP-induced nephrotoxicity in rat, and GTP pre-treatment was more effective than GTP post-treatment on reduction of CP-induced renal dysfunction.

The protective effect of pomegranate extract against cisplatin toxicity in rat liver and kidney tissue

“…These findings, suggestive of focal acute tubular necrosis, were compatible with the results of previous studies involving kidney damage induced with cisplatin (Ateşşahín et al, 2005(Ateşşahín et al, , 2007Ç ayır, 2011;Ahn et al, 2014).…”

“…Many fruit and vegetables are well-known for their powerful anti-oxidant activities. For that reason, various anti-oxidant agents have been investigated in red pepper (Shimeda et al, 2005), ginkgo biloba extract (Gulec et al, 2006), melatonin (Kilic et al, 2013), vitamin E (Kanter et al, 2007), vitamin C (Chen et al, 2014), lycopene (Atessahin et al, 2005, thymol (thyme oil) (Hosseinimehr et al, 2015), black tea (tannic acid) (Ahmed & Sultana, 2012), turmeric (curcumin, Indian saffron) (Sahin et al, 2014) and green tea (Ahn et al, 2014) for their protective effects against nephrotoxicity.…”

Potential of Pure Phytoconstituents and Herbs in Protection of Drug Induced Nephrotoxicity

“…The compounds such as catechin, epigallocatechin and epigallocatechin gallate (EGCG) found in green tea have been reported to be effective against nephrotoxicity. 12 A study carried out on mice with cisplatin-treatment showed that pre-treatment with polyphenols (EGCG and epigallocatechin) led to decline the toxic changes, cisplatin-induced side effects and improved renal function in mice 13 thus, it was also found that administration of 20-40 mg/kg of catechin and EGCG led to decline in tumour necrosis factor alpha (TNF-α) and MDA (malondialdehyde) and enhanced glutathione level.…”

Phytotherapy of nephrotoxicity-induced by cancer drugs: an updated review

“…Catechin, epigallocatechin-gallate, and epigallocatechin: The compounds found in green tea such as catechin, epigallocatechin gallate (EGCG), and epigallocatechin have been reported to be effective in protection against nephrotoxicity ( 45 ). A study on cisplatin-treated mice demonstrated that pretreatment with green tea polyohenols (EGCG, and epigallocatechin) decreased toxic changes and cisplatin-induced side effects and improved renal function in the mice under treatment ( 46 ). In addition, El-Mowafy et al found that administration of 20-40 mg/kg of green tea catechin and EGCG led to decrease in tumor necrosis factor alpha (TNF-α) and MDA and increased glutathione level.…”