Protective effects of growth hormone on bacterial translocation and intestinal damage in rats with partial intestinal obstruction

Abstract

Abstract:Objective: One of the reasons of bacterial translocation (BT) is the complete or partial intestinal obstructions (PIO) of the gastrointestinal system. In this study, we aimed to investigate the effects of recombinant human Growth Hormone (rhGH) on BT in rats with partial intestinal obstruction (PIO). Material and methods:The rats were randomly divided into the 4 groups: Group I: Sham-operated (SO) (n = 12), Group II control PIO (n = 12), Group III: PIO with rhGH treatment for 5 days (n = 12), Group IV: PIO with rhGH treatment 5 days before PIO and 5 days after PIO (a total of 10 days) (n = 12). In the groups III and IV, the effects of 5 and 10 days administered rhGH were examined. Results: The level of serum and of intestinal fl uid IgA was signifi cantly higher in the Group IV compared to the Group I, Group II and Group III. In the Group IV, the number of small intestinal goblet and colonic goblet cells, and the lengths of intestinal mucosal villi and crypt depths were statistically signifi cantly higher than in Groups II and III. The rate of bacterial translocation was higher in the Group II: 100 % in MLNs, 41.6 % in blood culture and 50.8 % in the liver cultures, it was signifi cantly higher compared to the other groups (p < 0.01). Conclusions:The study results demonstrated that administration of rhGH to the rats with PIO for at least 10 days decreased bacterial translocation (Fig. 3, Ref. 25). Text in PDF www.elis.sk.

Preventing Bacterial Translocation in Patients with Leaky Gut Syndrome: Nutrition and Pharmacological Treatment Options

“…Current literature provides a growing number of evidence from both animal and human studies that gastric acid influences the composition of the intestinal microbiome. Inhibition of gastric acid secretion by omeprazole predispose to duodenal bacterial overgrowth, which might potentially favor BT [ 161 , 162 , 163 , 164 ]. Similar changes were also observed during H2-antagonists therapy such as cimetidine; however, the effects were weaker [ 165 ].…”

Characteristics of Gut Microbiota in Patients with GH-Secreting Pituitary Adenoma

“…The growth hormone (GH)/insulin-like growth factor 1 (IGF-1) axis plays a vital role in regulating intestinal homeostasis in an individual ( 9 ). Both clinical trials and animal experimentation have demonstrated that exogenous recombinant human GH (rhGH) administration improves macronutrient absorption and decreases intestinal damage and bacterial translocation under the condition of short bowel syndrome ( 10 – 13 ). Using an intestinal epithelial-specific GH receptor knockout mouse model, Young et al verified that disruption of GH affects the intestinal gross anatomy, morphology, and function ( 14 ).…”