Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease

Zhu, Deliang; Xie, Mengyuan; Gademann, Fabian; Cao, Jixing; Wang, Peiyuan; Guo, Yanjie; Zhang, Lan; Su, Ting; Zhang, Jun; Chen, Jiansu

doi:10.1186/s13287-020-01608-8

Cited by 40 publications

(29 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Morphological evaluation also suggested that the transplanted RSCs were integrated into the host retina, implying its potential to substitute for the damaged cells. This observation was consistent with the previous reports [ 35 , 36 ]. The preliminary data from ERG further demonstrated the potential repair ability of RSCs, which was restricted in the outer layer of the damaged retina.…”

Section: Discussionsupporting

confidence: 94%

Transplanted embryonic retinal stem cells have the potential to repair the injured retina in mice

et al. 2021

View full text Add to dashboard Cite

Background Stem cell transplantation has been reported as one of the promising strategies to treat retinal degenerative diseases. But, the application and the role of retina stem cells (RSCs) in the treatment of patients with retinal degenerative diseases have not been fully revealed. This study aimed to investigate the potential role of transplantation of the embryo-derived RSCs into the vitreous cavity in repairing the damaged retina in mice. Methods RSCs were isolated from Kunming mice E17 embryonic retina and ciliary body tissues, and labeled with 5-bromo-2’-deoxyuridin (BrdU). Retinal optic nerve crush injury was induced in left eyes in male Kunming mice by ring clamping the optic nerve. The 6th -generation of BrdU-labeled RSCs were transplanted into the damaged retina by the intravitreal injection, and saline injected eyes were used as the control. Hematoxylin and eosin histological staining, and BrdU, Nestin and Pax6 immunostaining were performed. Electroretinogram (ERG) was used for assessing the electrical activity of the retina. Results Embryo-derived RSCs were identified by the positive stains of Pax6 and Nestin. BrdU incorporation was detected in the majority of RSCs. The damaged retina showed cellular nuclear disintegration and fragmentation in the retinal tissue which progressed over the periods of clamping time, and decreased amplitudes of a and b waves in ERG. In the damaged retina with RSCs transplantation, the positive staining for BrdU, Pax6 and Nestin were revealed on the retinal surface. Notably, RSCs migrated into the retinal ganglion cell layer and inner nuclear. Transplanted RSCs significantly elevated the amplitudes of a waves in retina injured eyes. Conclusions Embryonic RSCs have similar characteristics to neural stem cells. Transplantation of RSCs by intravitreal injection would be able to repair the damaged retina.

show abstract

Section: Discussionsupporting

confidence: 94%

Transplanted embryonic retinal stem cells have the potential to repair the injured retina in mice

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Morphological evaluation also suggests that the transplanted RSCs were integrated into the host retina, implying its potential to substitute for the damaged cells. This observation is consistent with previous reports [36,37]. However, differentiation of the transplanted RSCs was not distinguishable through light microscopy.…”

Section: Discussionsupporting

confidence: 93%

Transplanted Embryonic Retinal Stem Cells have the Potential to Repair the Injured Retina in Mice

Feng

Chen

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Stem cell transplantation has been reported as one of the promising strategies to treat retinal degenerative diseases, but this field is so complicated and still relatively new. This study investigated the potential role of transplantation of the embryo-derived retina stem cells (RSCs) into the vitreous cavity in repairing the damaged retina in mice.Methods: RSCs were isolated from Kunming mice E17 embryonic retina and ciliary body tissues. RSCs were cultured, differentiated, and labeled with 5-bromo-2’-deoxyuridin (BrdU). Retinal injury was induced in left eyes in male Kunming mice by ring clamping of the optic nerve. The 6th-generation of BrdU-labeled RSCs were transplanted into the damaged retina by the intravitreal injection, and saline injected eyes were used as the control. Hematoxylin and eosin histological staining, and BrdU, Nestin and Pax6 immunostaining were performed.Results: Embryo-derived RSCs were identified by the positive stains of Pax6 and Nestin. BrdU incorporation was detected in most of RSCs. The damaged retina showed diffuse edema, disordered and loose-arranged morphological changes in all retinal layers. In the damaged retina with RSCs transplantation, the positive staining for BrdU, Pax6 and Nestin were revealed on the retinal surface. Notably, a small amount of RSCs migrated into the retinal ganglion cell layer and inner nuclear.Conclusions: Embryonic RSCs have similar characteristics to neural stem cells. Transplantation of RSCs by intravitreal injection would be able to repair the damaged retina.

show abstract

“…Morphological evaluation also suggested that the transplanted RSCs were integrated into the host retina, implying its potential to substitute for the damaged cells. This observation was consistent with the previous reports [35,36]. The preliminary data from ERG further demonstrated the potential repair ability of RSCs, which was restricted in the outer layer of the damaged retina.…”

Section: Discussionsupporting

confidence: 93%

Transplanted embryonic retinal stem cells have the potential to repair the injured retina in mice

Feng

Chen

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Stem cell transplantation has been reported as one of the promising strategies to treat retinal degenerative diseases. But, the application and the role of retina stem cells (RSCs) in the treatment of patients with retinal degenerative diseases have not been fully revealed. This study aimed to investigate the potential role of transplantation of the embryo-derived RSCs into the vitreous cavity in repairing the damaged retina in mice. Methods: RSCs were isolated from Kunming mice E17 embryonic retina and ciliary body tissues, and labeled with 5-bromo-2’-deoxyuridin (BrdU). Retinal injury was induced in left eyes in male Kunming mice by ring clamping the optic nerve. The 6th-generation of BrdU-labeled RSCs were transplanted into the damaged retina by the intravitreal injection, and saline injected eyes were used as the control. Hematoxylin and eosin histological staining, and BrdU, Nestin and Pax6 immunostaining were performed. Electroretinogram (ERG) was used for assessing the electrical activity of the retina. Results: Embryo-derived RSCs were identified by the positive stains of Pax6 and Nestin. BrdU incorporation was detected in the majority of RSCs. The damaged retina showed cellular nuclear disintegration and fragmentation in the retinal tissue which progressed over the periods of clamping time, and decreased amplitudes of a and b waves in ERG. In the damaged retina with RSCs transplantation, the positive staining for BrdU, Pax6 and Nestin were revealed on the retinal surface. Notably, a small amount of RSCs migrated into the retinal ganglion cell layer and inner nuclear. Transplanted RSCs significantly elevated the amplitudes of a waves in retina injured eyes. Conclusions: Embryonic RSCs have similar characteristics to neural stem cells. Transplantation of RSCs by intravitreal injection would be able to repair the damaged retina.

show abstract

Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease

Cited by 40 publications

References 43 publications

Transplanted embryonic retinal stem cells have the potential to repair the injured retina in mice

Transplanted embryonic retinal stem cells have the potential to repair the injured retina in mice

Transplanted Embryonic Retinal Stem Cells have the Potential to Repair the Injured Retina in Mice

Transplanted embryonic retinal stem cells have the potential to repair the injured retina in mice

Contact Info

Product

Resources

About