Protective effects of<i>Bifidobacterium adolescentis</i>on collagen-induced arthritis in rats depend on timing of administration

Fan, Zhexin; Yang, Bo; Ross, R. Paul; Stanton, Catherine; Shi, Guo-Xun; Zhao, Jianxin; Zhang, Hao; Zhao, Jianxin

doi:10.1039/d0fo00077a

Cited by 38 publications

(30 citation statements)

References 53 publications

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“…The onset of RA leads to the release of pro-inflammatory cytokines, such as TNF-α, IL-6, and IL-1β (29). On the other hand, inflammatory cytokines [TNF-α, IL-6, IL-1β, or granulocyte-macrophage colony-stimulating factor (GM-CSF)] induce the synovitis inflammatory cascade, including synovial hypertrophy, and cartilage and bone invasion (30). Additionally, IL-1β mediates synoviocyte-induced cell proliferation and cartilage degradation (31).…”

Section: A B C D E Fmentioning

confidence: 99%

Ginkgolide B attenuates collagen-induced rheumatoid arthritis and regulates fibroblast-like synoviocytes-mediated apoptosis and inflammation

et al. 2020

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Background: Rheumatoid arthritis (RA) is a systemic disease characterized by chronic synovial infiltration and proliferation, cartilage destruction, and joint injury. Ginkgolide B (GB) is an extract of the leaves of Ginkgo biloba, and pharmacological studies have shown that it has anti-inflammatory and anti-apoptotic activities. The purpose of this study was to investigate the anti-RA properties of GB. Methods: In vivo, we established a collagen II-induced arthritis (CIA) mouse model. Mice were divided into five groups (n=10): sham, CIA, GB (10 µM), GB (20 µM), and GB (40 µM). We measured arthritis score, synovial histopathological change, and peripheral blood cytokine levels. In vitro, we used lipopolysaccharide (LPS)-induced-fibroblast-like synoviocytes (RA-FLSs) as the study subject. Cell viability, apoptosis, and inflammatory cytokines levels were detected by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, flow cytometry, and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), respectively. Finally, the protein expression of wingless-type family member 5A (Wnt5a), c-Jun N-terminal kinase (JNK), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 were detected by Western blot.Results: Arthritis scores, synovial hyperplasia, and cartilage and bone destruction were significantly ameliorated by GB. Additionally, GB decreased the serum levels of interleukin (IL)-1β, IL-6, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor alpha (TNF-α), matrix metalloproteinase (MMP)-3 and MMP-13, and increased IL-10. In vitro, we found that GB remarkably inhibited RA-FLSs viability at 24 or 48 h in a concentration-dependent manner. The apoptotic ratio was reduced by GB, and it increased the expression of cleaved-Caspase-3 and Bax while decreasing Bcl-2 expression in RA-FLSs. Furthermore, GB attenuated the progression of inflammation by mediating inflammatory cytokine release and MMPs gene expression. Meanwhile, GB inactivated the expression levels of Wnt5a, phosphorylated (p)-JNK, and p-P65 in the synovial tissues and RA-FLSs.Conclusions: This study was the first to demonstrate that the anti-RA effect of GB is related to reducing articular cartilage and bone destruction, inducing RA-FLSs apoptosis, and regulating inflammatory cytokine release and the Wnt5a/JNK/NF-κB axis. All the findings highlight that GB might provide a novel treatment approach for RA.

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Section: A B C D E Fmentioning

confidence: 99%

Ginkgolide B attenuates collagen-induced rheumatoid arthritis and regulates fibroblast-like synoviocytes-mediated apoptosis and inflammation

et al. 2020

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“…Early probiotic administration performed better in promoting SCFAs production, had significant higher Tregs cells frequency and lower levels of TNF compared to the late B. adolescentis treated group. Moreover, SCFA positively correlated with Tregs and negatively correlated with pro-inflammatory cytokines in the early treated group (Fan et al, 2020). These findings suggest that the introduction of B. adolescentis before arthritis can impact the onset of arthritic inflammation, and support that GM manipulating therapies should be provided at an early stage of the disease or even before disease occurrence.…”

Section: Studies With the Collagen-induced Arthritis Animal Modelmentioning

confidence: 57%

“…A study conducted by Fan et al compared the effects of a preventive and therapeutic treatment with Bifidobacterium adolescentis (B. adolescentis) in CIA rats (Fan et al, 2020). Preventive B. adolescentis administration had better results in reducing the clinical symptoms, rebalancing the pro-and antiinflammatory responses, and reversing the gut dysbiosis than late B. adolescentis treatment.…”

Section: Studies With the Collagen-induced Arthritis Animal Modelmentioning

confidence: 99%

“…We have considered both prophylactic (when probiotic administration started before immunization or before arthritis onset) and therapeutic (when dosing with study probiotic started after clinical signs of disease) treatment regimens with probiotic bacteria in CIA and AIA models. A number of preclinical studies reported the beneficial effects of probiotics via multiple pathways, including restoring the dysbiosis of GM in a prophylactic way (Achi et al, 2019;Pan et al, 2019;Fan et al, 2020). A study conducted by Liu et al, has reported significant differences in the microbiome composition of CIA-susceptible and CIA-resistant mice (Liu et al, 2016).…”

Section: Summary Of Evidence From Animal Modelsmentioning

confidence: 99%

“…These findings highlighted the importance of monitoring changes in microbial communities during disease progression and provided powerful evidence to explain the evolution of the GM in RA. Of interest, Fan et al reported the beneficial effects of early treatment in maintaining gut microbial communities (Fan et al, 2020).…”

Section: Summary Of Evidence From Animal Modelsmentioning

confidence: 99%

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Probiotic Supplementation for Rheumatoid Arthritis: A Promising Adjuvant Therapy in the Gut Microbiome Era

et al. 2021

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Rheumatoid arthritis (RA) is a chronic immune-mediated inflammatory disease that ultimately leads to joint destruction and functional disability. Although the exact etiology of RA is not fully understood, it is well established that gut microbiota (GM) plays a vital role in the pathogenesis of RA, with accumulating evidence suggesting that gut dysbiosis induces a chronic inflammatory response that may be linked to disease development. Of interest, patients with RA have significant changes in the intestinal microbiota compared to healthy controls, and several studies have suggested the use of probiotics as a possible adjuvant therapy for RA. Benefits of probiotic supplementation were reported in animal models of arthritis and human studies, but the current evidence regarding the effect of probiotic supplementation in the management of RA remains insufficient to make definite recommendations. Several different strains of Lactobacillus and Bifidobacteria, as single species or in mixed culture, have been investigated, and some have demonstrated beneficial effects on disease activity in RA human subjects. As of now, L.casei probiotic bacteria seems to be the strongest candidate for application as adjuvant therapy for RA patients. In this review, we highlight the role of GM in the development and progression of RA and summarize the current knowledge on the use of probiotics as a potential adjuvant therapy for RA. We also review the proposed mechanisms whereby probiotics regulate inflammation. Finally, the role of fermented foods is discussed as a possible alternative to probiotic supplements since they have also been reported to have health benefits.

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Impact of dietary live microbes and nondietary prebiotic/probiotic intake on osteoarthritis and rheumatoid arthritis development: Stratified findings from NHANES data

Liu,

Zhang,

Liang

et al. 2024

iMetaOmics

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We selected participants from the NHANES database from 2005 to 2016 for this cross‐sectional analysis. Logistic regression and other analytical methods were utilized to analyze the relationship between the intake of dietary live microbes and nondietary prebiotic/probiotic and the prevalence of osteoarthritis (OA) and rheumatoid arthritis (RA). The findings demonstrated a direct relationship between the consumption of nondietary prebiotic/probiotic and the prevalence of developing OA, whereas a greater consumption of dietary live microbes is associated with a lower occurrence of RA. (Graphical abstract was created with https://BioRender.com).

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Protective effects ofBifidobacterium adolescentison collagen-induced arthritis in rats depend on timing of administration

Abstract: Early probiotics adminitration is a key timing to alleviate inflammation in rats with arthritis.

Cited by 38 publications

References 53 publications

Ginkgolide B attenuates collagen-induced rheumatoid arthritis and regulates fibroblast-like synoviocytes-mediated apoptosis and inflammation

Ginkgolide B attenuates collagen-induced rheumatoid arthritis and regulates fibroblast-like synoviocytes-mediated apoptosis and inflammation

Probiotic Supplementation for Rheumatoid Arthritis: A Promising Adjuvant Therapy in the Gut Microbiome Era

Impact of dietary live microbes and nondietary prebiotic/probiotic intake on osteoarthritis and rheumatoid arthritis development: Stratified findings from NHANES data

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