Protective effects of icariin on human vascular endothelial cells induced by oxidized low-density lipoprotein via modulating caspase-3 and Bcl-2

Hu, Yanwu; Li, Haitao; Liu, Kai; Zhang, Yang; Ren, Liqun; Zhang, Fan

doi:10.3892/mmr.2018.8717

Cited by 8 publications

(9 citation statements)

References 36 publications

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“…Consequently, it is cleaved and activated during late apoptotic events [27]. A previous study reported that ICA prevents apoptosis in human vascular endothelial cells following exposure to oxidized low-density lipoprotein by regulating expression of BCL-2 and CASP3 protein and mRNA [28]. It addition, ICA decreased the expression of pro-apoptotic protein (cleaved caspase3 and cleaved poly (ADP-ribose) polymerase), by inhibiting the apoptotic signaling pathway in K562 cell exposed to radiation [29].…”

Section: Wwwanimbiosciorg 553mentioning

confidence: 99%

The antioxidant icariin protects porcine oocytes from age-related damage in vitro

et al. 2021

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Objective: If fertilization does not occur within a specific period, the quality of unfertilized oocytes in the oviduct (in vivo aging) or in culture (in vitro aging) will deteriorate over time. Icariin (ICA), found in all species of Epimedium herbs, has strong antioxidant activity, and is thought to exert anti-aging effects in vitro. We asked whether ICA protects oocytes against age-related changes in vitro.Methods: We analyzed the reactive oxygen species (ROS) levels and expression of antioxidant, maternal, and estrogen receptor genes, and along with spindle morphology, and the developmental competence and quality of embryos in the presence and absence of ICA.Results: Treatment with 5 μM ICA (ICA-5) led to a significant reduction in ROS activity, but increased mRNA expression of glutathione and antioxidant genes (superoxide dismutase 1 [SOD1], SOD2, peroxiredoxin 5, and nuclear factor erythroid 2‐like 2), during aging in vitro. In addition, ICA-5 prevented defects in spindle formation and chromosomal alignment, and increased mRNA expression of cytoplasmic maturation factor genes (bone morphogenetic protein 15, cyclin B1, MOS proto‐oncogene, serine/threonine kinase, and growth differentiation factor‐9). It also prevented apoptosis, increased mRNA expression of antiapoptotic genes (BCL2-like 1 and baculoviral IAP repeat-containing 5), and reduced mRNA expression of pro-apoptotic genes (BCL2 antagonist/killer 1 and activation of caspase-3). Although the maturation and cleavage rates were similar in all groups, the total cell number per blastocyst and the percentage of apoptotic cells at the blastocyst stage were higher and lower, respectively, in the control and ICA-5 groups than in the aging group.Conclusion: ICA protects oocytes against damage during aging in vitro; therefore, it can be used to improve assisted reproductive technologies.

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Section: Wwwanimbiosciorg 553mentioning

confidence: 99%

The antioxidant icariin protects porcine oocytes from age-related damage in vitro

et al. 2021

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“…The study was designed to elucidate whether ER stress plays an essential role in ICA-induced protection of vascular endothelial dysfunction and explore possible signaling mechanisms involved under diabetes conditions. We used a rat model of STZ-induced dia- Several studies have reported that ICA has the effect of attenuating endothelial dysfunction (Hu et al, 2018;Sun et al, 2019;Tang et al, 2015;Xiao et al, 2015). Vascular reactivity study showed that ICA significantly improved Ach-induced vasodilation and vascular contraction responses to Phe in diabetic aortas compared with nontreated diabetic aortas, whereas SNP induced endotheliumindependent relaxation was similar among the groups.…”

Section: Discussionmentioning

confidence: 98%

“…Several studies have reported that ICA has the effect of attenuating endothelial dysfunction (Hu et al, 2018; Sun et al, 2019; Tang et al, 2015; Xiao et al, 2015). Vascular reactivity study showed that ICA significantly improved Ach‐induced vasodilation and vascular contraction responses to Phe in diabetic aortas compared with nontreated diabetic aortas, whereas SNP induced endothelium‐independent relaxation was similar among the groups.…”

Section: Discussionmentioning

confidence: 99%

“…A previous investigation showed that ICA could restrain human umbilical vein endothelial cells (HUVECs) to improve endothelial function by the regulation of the PRMT/ADMA/DDAH pathway (Xiao, Liu, Lu, Deng, & Luo, 2015). Hu et al (2018) proved that ICA improved HUVECs damage and apoptosis induced by ox‐LDL. Sun et al (2019) first reported that ICA treatment was beneficial to protect against high glucose (HG)‐induced endothelial dysfunction, through reducing apoptosis, oxidative stress, and inflammation.…”

Section: Introductionmentioning

confidence: 99%

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Icariin ameliorates endothelial dysfunction in type 1 diabetic rats by suppressing ER stress via the PPARα/Sirt1/AMPKα pathway

Yao

Wang

et al. 2020

Journal Cellular Physiology

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Icariin (ICA), as a flavonoid glycoside, is associated with the improvement of vascular complications in diabetes. However, its protective mechanisms remain to be wellestablished. Here, we tested the hypothesis that ICA attenuates vascular endothelial dysfunction by inhibiting endoplasmic reticulum (ER) stress in type 1 diabetes. In streptozotocin-induced diabetic rats, ICA positively affected acetylcholine-induced vasodilation and phenylephrine-induced vasoconstriction in aortas. ICA treatment significantly attenuated ER stress in diabetic rats and high-glucose induced human umbilical vein endothelial cells. Incubation with ICA in vitro attenuated vascular reactivity in diabetic rats, which was blocked by the ER stress inducer, and peroxisome proliferator-activated receptor α (PPARα), sirtuin1 (Sirt1), or AMP-activated protein kinase-α (AMPKα) inhibitors. Western blot showed that ICA activated the PPARα/Sirt1/AMPKα pathway, which contributed to reducing ER stress and activating endothelial nitric oxide synthase in vivo and vitro. Our results implicate that ICA normalizes ER stress to attenuate endothelial dysfunction by the regulation of the PPARα/Sirt1/AMPKα pathway.

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“…Pieces of evidence have shown that ICA appears to be beneficial for AS in vivo and in vitro models [9–11]. Our recent study demonstrated that ICA was a concentration-dependent agent in protecting human vascular endothelial cells (HUVECs) against ox-LDL-induced injury [12]. However, the detailed molecular mechanisms of its therapeutic capacities remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Effects of icariin on long noncoding RNA and mRNA expression profile in the aortas of apoE-deficient mice

Zhang

et al. 2019

Bioscience Reports

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Objective : The beneficial effects of icariin (ICA) in ameliorating atherosclerosis (AS) are well known, but the underlying protective mechanism has not been fully elucidated. The present study aimed to investigate altered long noncosing RNA (lncRNA) and mRNA expression profiles in ApoE−/− mice after ICA treatment. Method : The atherosclerotic plaque area was evaluated on high-fat diet (HFD)-induced ApoE−/− mice treated with either ICA or vehicle. LncRNA and mRNA integrated microarrays was performed on aortic tissues. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were utilized to explore the significant function and pathway of the differentially expressed (DE) mRNAs, global signal transduction network were constructed to select key mRNAs, and lncRNA–mRNA co-expression network was built to find out the interactions between lncRNA and mRNA. Quantitative real-time PCR (qPCR) was used to further validate the expressions of selected lncRNAs and mRNAs. Results : Administration of ICA significantly reduced plaque size after 12 weeks (P<0.05). A total of 1512 DE lncRNAs and 2059 DE mRNAs were identified. The mRNAs: protein kinase C, β (Prkcb), Cyp2c65, Mapk10, Calmodulin 5 (Calm5), Calmodulin-like 3 (Calml3) and Camk4 were selected as hub mRNAs, the correlated lncRNAs in co-expression network were identified as important regulatory lncRNAs. The identified target pairs such as lncRNA-NONMMUT000659/Prkcb may play critical roles in AS development mediated by ICA. Conclusion : Taken together, our study highlights a panel of DE lncRNAs and mRNAs that could explain the molecular mechanism of ICA’s anti-atherosclerotic effects. The work lays a foundation for subsequent genes functional researches, which could contribute to provide new therapeutic targets for AS.

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Protective effects of icariin on human vascular endothelial cells induced by oxidized low-density lipoprotein via modulating caspase-3 and Bcl-2

Cited by 8 publications

References 36 publications

The antioxidant icariin protects porcine oocytes from age-related damage in vitro

The antioxidant icariin protects porcine oocytes from age-related damage in vitro

Icariin ameliorates endothelial dysfunction in type 1 diabetic rats by suppressing ER stress via the PPARα/Sirt1/AMPKα pathway

Effects of icariin on long noncoding RNA and mRNA expression profile in the aortas of apoE-deficient mice

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