Protective effects of leonurine hydrochloride on pyroptosis in premature ovarian insufficiency via regulating NLRP3/GSDMD pathway

Chi, Yan-Nan; Hai, Dong-Mei; Ma, Lin; Cui, Yanhong; Hu, Haitao; Liu, Ning; Juan-Du,; Lan, Xiao-Bing; Yang, Jiamei

doi:10.1016/j.intimp.2022.109520

Cited by 15 publications

(9 citation statements)

References 26 publications

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“…Therefore, it can be used as a quality marker for qualitative identification and content determination ( Xiong and Peng, 2016 ). It also has the pharmacological effects of diuresis, anti-platelet aggregation, anti-oxidation, inhibition of creatine kinase activity, and inhibition of the vascular smooth muscle contractile response to vasoconstrictors ( Chi et al, 2022 ; Shen et al, 2023 ). The results showed that the level of leonurine hydrochloride in the control group was significantly and much higher than the standard level, that in the non-fertilization group was just at the standard level, and that in the drought group was far below the content standard.…”

Section: Discussionmentioning

confidence: 99%

A biophoton method for identifying the quality states of fresh Chinese herbs

et al. 2023

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Introduction: The quality of Chinese herbs is the basis for ensuring their safety and efficacy. However, the quality evaluation system is imperfect. In particular, there is a lack of quality evaluation methods for fresh Chinese herbs during growth. The biophoton is a common phenomenon and provides complete information about the interior of the living system, which is consistent with the holistic concept of traditional Chinese medicine. Therefore, we aim to correlate the biophoton characteristics with the quality states to find the biophoton parameters that can characterize the quality states of fresh Chinese herbs.Methods: The biophoton characteristics of motherwort and safflower were measured and characterized by the counts per second (CPS) in the steady state and the initial intensity (I0) and coherent time (T) of delayed luminescence. The active ingredient content was measured by ultra-high-performance liquid chromatography (UPLC). The pigment content of motherwort leaves was measured by UV spectrophotometry. The t-test and correlation analysis were performed on the experimental results.Results: The CPS and I0 of motherwort and I0 of safflower showed a significant downward trend during the growth process, and their active ingredient content showed a trend that increased and then decreased. The CPS, I0, and the content of active ingredients and pigments in a healthy state were significantly higher than those in a poor state, while T showed the opposite results. The CPS and I0 were all significantly and positively correlated with the content of active ingredients and pigments, while the T of motherwort showed the opposite results.Conclusion: It is feasible to identify the quality states of fresh Chinese herbs by using their biophoton characteristics. Both CPS and I0 have better correlations with the quality states and can be considered characteristic parameters of the quality of fresh Chinese herbs.

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Section: Discussionmentioning

confidence: 99%

A biophoton method for identifying the quality states of fresh Chinese herbs

et al. 2023

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“…Furthermore, in the presence of pathogenic infection or the stimulation of danger signals, the occurrence of preterm PROM relies on fetal membrane cells initiating classical and/or nonclassical pathways of cell apoptosis. [ 26 , 27 ] In these pathways, the activation of Caspase-1 and Caspase-3 promotes the maturation of IL-1 and acts upon the substrate Gasdermin family D protein (GSDMD). The role of GSDMD in microbial-induced inflammatory responses has been extensively explored.…”

Section: Discussionmentioning

confidence: 99%

Exploratory study on the mechanism of necrotic effect of nourishing cells in the context of genital tract infection in premature rupture of membranes

Qian,

et al. 2023

Medicine

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To explore the mechanism of necrotic effect of nourishing cells in the context of genital tract infection in premature rupture of membranes (PROM). One hundred eight patients with PROM treated at our hospital from June 2020 to June 2022 were selected as the PROM group. Simultaneously, 108 cases of normal full-term pregnant women were chosen as the control group. Western blot analysis was performed to measure the relative expression levels of cysteinyl aspartate specific proteinase-1 (Caspase-1), cysteinyl aspartate specific proteinase-3 (Caspase-3), nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3), and interleukin (IL)-1β proteins, which are associated with necrosis of placental nourishing cells, in the placenta of both groups. TUNEL staining was used to detect the number of apoptotic placental nourishing cells. The differences in necrotic factors of placental nourishing cells were analyzed between full-term and preterm cases in the PROM group, as well as among patients with different genital tract infections. The apoptotic count of placental nourishing cells in the PROM group was 58.46 ± 11.26 cells/field, which was markedly higher than that of the control group (P < .05). The relative expression levels of the necrotic factors Caspase-1, Caspase-3, NLRP3, and IL-1β proteins in placental nourishing cells of the PROM group were 1.32 ± 0.26, 1.19 ± 0.30, 1.29 ± 0.28, and 1.23 ± 0.24, respectively. These values were significantly higher than those of the control group (P < .05). The relative expression levels of the necrotic factors Caspase-1, Caspase-3, NLRP3, and IL-1β proteins in placental nourishing cells were compared between full-term and preterm patients in the PROM group (P > .05). The relative expression levels of the necrotic factors Caspase-1, Caspase-3, NLRP3, and IL-1β proteins in placental nourishing cells were higher in patients with multiple genital tract infections compared to those with single infections or no infections in the PROM group (P < .05). PROM is associated with a significant upregulation of placental nourishing cell apoptosis and necrotic factors, including Caspase-1, Caspase-3, NLRP3, and IL-1β proteins. This upregulation is correlated with the presence of genital tract infections.

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“…Pyroptosis, triggered by inflammation and exacerbates inflammatory responses, is believed to play a crucial role in SIC [ 4 ]. The NOD-like receptor protein 3 (NLRP3) inflammasome/Caspase-1/ GSDMD pathway has been identified as a classical pathway involved in regulating pyroptosis [ 5 ], and recent studies have demonstrated that inhibition of GSDMD-dependent pyroptosis can improve cardiac dysfunction in septic cardiomyopathy [ 6 ]. In addition, the suppression of inflammation, cell apoptosis and pyroptosis by blocking Toll-like receptor 4 (TLR4) signaling and NLRP3 inflammasome pathways has been shown to alleviate myocardial dysfunction in sepsis [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

IP3R2-mediated Ca2+ release promotes LPS-induced cardiomyocyte pyroptosis via the activation of NLRP3/Caspase-1/GSDMD pathway

Wu,

Yang,

Zhang

et al. 2024

Cell Death Discov.

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Pyroptosis plays a crucial role in sepsis, and the abnormal handling of myocyte calcium (Ca2+) has been associated with cardiomyocyte pyroptosis. Specifically, the inositol 1,4,5-trisphosphate receptor type 2 (IP3R2) is a Ca2+ release channel in the endoplasmic reticulum (ER). However, the specific role of IP3R2 in sepsis-induced cardiomyopathy (SIC) has not yet been determined. Thus, this study aimed to investigate the underlying mechanism by which IP3R2 channel-mediated Ca2+ signaling contributes to lipopolysaccharide (LPS)—induced cardiac pyroptosis. The SIC model was established in rats by intraperitoneal injection of LPS (10 mg/kg). Cardiac dysfunction was assessed using echocardiography, and the protein expression of relevant signaling pathways was analyzed using ELISA, RT-qPCR, and western blot. Small interfering RNAs (siRNA) and an inhibitor were used to explore the role of IP3R2 in neonatal rat cardiomyocytes (NRCMs) stimulated by LPS in vitro. LPS-induced NLRP3 overexpression and GSDMD-mediated pyroptosis in the rats’ heart. Treatment with the NLRP3 inhibitor MCC950 alleviated LPS-induced cardiomyocyte pyroptosis. Furthermore, LPS increased ATP-induced intracellular Ca2+ release and IP3R2 expression in NRCMs. Inhibiting IP3R activity with xestospongin C (XeC) or knocking down IP3R2 reversed LPS-induced intracellular Ca2+ release. Additionally, inhibiting IP3R2 reversed LPS-induced pyroptosis by suppressing the NLRP3/Caspase-1/GSDMD pathway. We also found that ER stress and IP3R2-mediated Ca2+ release mutually regulated each other, contributing to cardiomyocyte pyroptosis. IP3R2 promotes NLRP3-mediated pyroptosis by regulating ER Ca2+ release, and the mutual regulation of IP3R2 and ER stress further promotes LPS-induced pyroptosis in cardiomyocytes.

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Protective effects of leonurine hydrochloride on pyroptosis in premature ovarian insufficiency via regulating NLRP3/GSDMD pathway

Cited by 15 publications

References 26 publications

A biophoton method for identifying the quality states of fresh Chinese herbs

A biophoton method for identifying the quality states of fresh Chinese herbs

Exploratory study on the mechanism of necrotic effect of nourishing cells in the context of genital tract infection in premature rupture of membranes

IP3R2-mediated Ca2+ release promotes LPS-induced cardiomyocyte pyroptosis via the activation of NLRP3/Caspase-1/GSDMD pathway

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