Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells

Zou, Xiulan; Yu, Yong-Zhen; Yu, Honghua; Wang, Guan‐Feng; Xu, Zhe; Zhang, Chu; Zhou, Wenjie; Li, Dandan; Chen, Xuan-Ge; Zou, Yao

doi:10.18240/ijo.2019.08.05

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…However, in heat-stress conditions, ALA significantly inhibited the increase in Bax and caspase 3 protein levels and promoted Bcl-2 protein expression. This is consistent with previous reports that ALA protects cells and tissues against oxidative damage and cell death, such as renal tubular epithelial cell injury in humans [45] and oxidative damage of human retinal pigment epithelial cells [46], by regulating the expression of these ER stress-related apoptosis genes. Therefore, we infer that the down-regulation of Bcl-2 and the up-regulation of Bax and caspase 3 may be the mechanism whereby ALA ameliorated heat stress-induced histological changes and oxidative stress in chicken testes.…”

Section: Discussionsupporting

confidence: 93%

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

Xiong

Yin

et al. 2020

Animals

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Heat stress (HS) causes testicular injury, resulting in decreased fertility. Alpha-lipoic acid (ALA) is a well-known antioxidant. The aim of this study was to investigate the protective effects of ALA on HS-induced testicular damage in chickens. Histological changes; biomarkers of oxidative stress, including glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA); markers of endoplasmic reticulum (ER) stress, including glucose-regulated protein 78 (GRP78) and CCAAT/enhancer binding protein homologous protein (CHOP); apoptosis-related modulators, including Bax, Bcl-2, and caspase 3, in testicular tissue and serum testosterone levels were evaluated in chickens under heat stress. Heat stress induces spermatogenic cell abnormalities in chicken testes. Compared to the HS group, the histomorphological abnormalities in testicular tissue were visibly ameliorated, with significant increases in the enzyme activities of GPx, SOD, and CAT, increased serum testosterone concentration, and decreased MDA levels in the ALA + HS group. Consistent with these results, compared with the HS group, the protein levels of GRP78, CHOP, caspase 3, and Bax were significantly decreased, whereas Bcl-2, StAR, and 3β-HSD protein levels were increased in the ALA + HS group. Collectively, these findings suggest that ALA significantly ameliorates the heat-induced histomorphological abnormalities in the testes and decreased testosterone production by potentiating the activities of anti-oxidative enzymes (GPx, SOD, and CAT), inhibiting ER stress-related apoptotic pathways (Bax, Bcl-2, and caspase 3), and increasing steroidogenic gene (StAR and 3β-HSD) expression in chickens.

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Section: Discussionsupporting

confidence: 93%

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

Xiong

Yin

et al. 2020

Animals

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“…2a,c). As mentioned before, a growing body of evidence indicates that mitochondria in RPE cells might be strongly affected by blue light (16,41,63). Therefore, it is possible that blue light treatment of ARPE‐19 cells could lead to mitochondria impairment and thereby disturbance of phagocytic function, a process that demands a large supply of energy from ATP produced by mitochondria (69).…”

Section: Resultsmentioning

confidence: 99%

“…1a,b). Since mitochondria play a critical role in the RPE cell damage mechanism induced by blue light (16,63), cytotoxic effects of blue light treatment were concurrently analyzed by the MTT assay for metabolic activity of cells (Fig. 1c).…”

Section: Blue Light Irradiation Of Arpe-19 Cells: Effect On Specific ...mentioning

confidence: 99%

The Inhibitory Effect of Blue Light on Phagocytic Activity by ARPE‐19 Cells

Olchawa

Herrnreiter

Skumatz

et al. 2022

Photochem & Photobiology

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Chronic exposure of the retina to short wavelength visible light is a risk factor in pathogenesis of age-related macular degeneration. The proper functioning and survival of photoreceptors depends on efficient phagocytosis of photoreceptor outer segments (POS) by retinal pigment epithelium. The purpose of this study was to analyze the phagocytic activity of blue light-treated ARPE-19 cells, and to examine whether the observed effects could be related to altered levels of POS phagocytosis receptor proteins and/or to oxidation of cellular proteins and lipids. POS phagocytosis was measured by flow cytometry. Phagocytosis receptor proteins av and b5 integrin subunits and Mer tyrosine kinase (MerTK) were quantified by western blotting. The intact functional heterodimer avb5 was quantified by immunoprecipitation followed by immunoblotting. Cellular protein and lipid hydroperoxides were analyzed by coumarin boronic acid probe and iodometric assay, respectively. Cell irradiation induced reversible inhibition of specific phagocytosis and transient reductions in phagocytosis receptor proteins. Full recovery of functional heterodimer was apparent. Significant photooxidation of cellular proteins and lipids was observed. The results indicate that transient inhibition of specific phagocytosis by blue light could be related to the reduction in phagocytosis receptor proteins. Such changes may arise from oxidative modifications of cell phagocytic machinery components.

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“…Then, the medium was replaced with DMEM/F12 (2% FBS) and DMEM low glucose (1% FBS). Cells were divided into two groups-(1) control: cells were cultured in a humidified atmosphere of 95% air and 5% CO 2 at 37 • C and covered by aluminum foil; (2) blue light exposure: cells illuminated with the blue light source at a distance to keep the light intensity at 2500 lux [46] for 3 h, 6 h, and 16 h. [43,47]. For cell protective assay, DN-P, DN-PP, and ALA were pretreated for 1 h before blue light exposure for 6 h.…”

Section: Time Course Study Of Blue Light Exposure In Arpe-19 and 661w...mentioning

confidence: 99%

Dendrobium nobile Polysaccharide Attenuates Blue Light-Induced Injury in Retinal Cells and In Vivo in Drosophila

Hsu,

Sangkhathat,

et al. 2024

Antioxidants

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Blue light is the higher-energy region of the visible spectrum. Excessive exposure to blue light is known to induce oxidative stress and is harmful to the eyes. The stems of Dendrobium nobile Lindl. (Orchidaceae), named Jinchaishihu, have long been used in traditional Chinese medicine (TCM) for nourishing yin, clearing heat, and brightening the eyes. The polysaccharide is one of the major components in D. nobile. However, the effect on ocular cells remains unclear. This study aimed to investigate whether the polysaccharide from D. nobile can protect the eyes from blue light-induced injury. A crude (DN-P) and a partially purified polysaccharide (DN-PP) from D. nobile were evaluated for their protective effects on blue light-induced damage in ARPE-19 and 661W cells. The in vivo study investigated the electroretinographic response and the expression of phototransduction-related genes in the retinas of a Drosophila model. The results showed that DN-P and DN-PP could improve blue light-induced damage in ARPE-19 and 661W cells, including cell viability, antioxidant activity, reactive oxygen species (ROS)/superoxide production, and reverse opsin 3 protein expression in a concentration-dependent manner. The in vivo study indicated that DN-P could alleviate eye damage and reverse the expression of phototransduction-related genes, including ninaE, norpA, Gαq, Gβ76C, Gγ30A, TRP, and TRPL, in a dose-dependent manner in blue light-exposed Drosophila. In conclusion, this is the first report demonstrating that D. nobile polysaccharide pretreatment can protect retinal cells and retinal photoreceptors from blue light-induced damage. These results provide supporting evidence for the beneficial potential of D. nobile in preventing blue light-induced eye damage and improving eyesight.

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Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells

Cited by 5 publications

References 25 publications

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

The Inhibitory Effect of Blue Light on Phagocytic Activity by ARPE‐19 Cells

Dendrobium nobile Polysaccharide Attenuates Blue Light-Induced Injury in Retinal Cells and In Vivo in Drosophila

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