Protective effects of methanolic extract of Adhatoda vasica Nees leaf in collagen-induced arthritis by modulation of synovial toll-like receptor-2 expression and release of pro-inflammatory mediators

Adhikary, Rana; Majhi, Arnab; Mahanti, Sayantika; Bishayi, Biswadev

doi:10.1016/j.jnim.2015.11.001

Cited by 14 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, IL-10 ameliorates RA by downregulating proinflammatory cytokine expression [ 41 ]. Stimulation of PMNs enhances production of free radicals and proinflammatory cytokines in inflamed synovial tissue, possibly leading to elevated serum levels of proinflammatory cytokines [ 42 ]. IL-17 (Th 17) promotes recruitment of neutrophils/macrophages (infiltration) and thus results in synovial joint tissue damage and, subsequently, RA [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Salvianolic Acid B Suppresses Inflammatory Mediator Levels by Downregulating NF-κB in a Rat Model of Rheumatoid Arthritis

et al. 2018

View full text Add to dashboard Cite

BackgroundSalvianolic acid B (SB) is a major active phyto-component of the plant Radix Salvia miltiorrhiza, which is traditionally used to treat joint pain and arthritis. The present study examined the anti-rheumatoid arthritis efficacy of SB on collagen-induced rheumatoid arthritis (CIA) in a rat model.Material/MethodsForty-eight rats were divided into 4 groups: Control rats treated with saline (Group I), rats subjected to CIA induction by intradermal injection of bovine collagen II type at the tail (Group II), and rats subjected to CIA and supplemented with either 20 or 40 mg/kg of SB for 28 days (group III or IV).ResultsPaw swelling, edema, arthritis score, thymus and spleen indexes, and neutrophil infiltration were significantly decreased (p<0.01) by treatment with 20 or 40 mg/kg of SB. The levels of inflammatory cytokines (interleukin-1β, -6, and -17, and TNF-α) and anti-collagen II-specific immunoglobulins (IgG1 and IgG2a) were markedly decreased (p<0.01), and those of antioxidant enzymes (SOD, CAT, and GSH) were significantly increased (p<0.01) in SB-treated rats. Administration with SB (20 or 40 mg/kg) resulted in lower phosphorylated IκB-α and NF-κB p65 protein levels and markedly downregulated IκB-α expression. Furthermore, CIA rats revealed the presence of highly diffused polymorphonuclear cells (PMNs) infiltration with eroded cartilage; however, these phenomena were considerably ameliorated by SB.ConclusionsSB alleviates oxidative stress and inflammation in CIA rats, thus verifying its anti-rheumatoid arthritis property.

show abstract

Section: Discussionmentioning

confidence: 99%

Salvianolic Acid B Suppresses Inflammatory Mediator Levels by Downregulating NF-κB in a Rat Model of Rheumatoid Arthritis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…7 Many commercially available steroids and non-steroidal anti-inflammatory drugs have a boundless influence in the cure for inflammatory diseases, but due to their indeterminate side effects there arises a need for lead targets with the minimal adverse effect. 8,9 Folklore medicinal plants with traditional medicinal values provide enormous health care benefits with new therapies and fewer side effects. The World Health Organization (WHO) estimated that 80% of the world population depends on these customary herbal plants for their health essentials due to their abundance and varied bioactivities compared to other commercial available non-steroidal anti-inflammatory drugs.…”

Section: Cell Viability Assessment Of Alk-f Of a Vasica In Raw 2647mentioning

confidence: 99%

Presence of pyrroloquinazoline alkaloid in Adhatoda vasica attenuates inflammatory response through the downregulation of pro-inflammatory mediators in LPS stimulated RAW 264.7 macrophages

Reddy¹,

Sujatha²

2019

Bioimpacts

View full text Add to dashboard Cite

Introduction: Inflammation is the primary response caused due to harmful stimuli which are followed by the increased draining of plasma and immune cells from the body into the site of the injured tissue. A signaling cascade of growth factors and cytokines propagates and eventually matures in the inflammatory site involving the blood vessels and immune markers within the injured tissue in order to promote the renewal of the degenerated tissue. During a chronic disorder like diabetic foot ulcer, there is an obstinate inflammation which may act as a prime factor for limb amputation and upon persistent prevalence may even lead to death. Methods: This study focuses on the mode of action of ALK-F (alkaloid fraction) isolated from Adhatoda vasica in attenuating the nitric oxide production which was estimated by Griess assay, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) expression was analyzed by ELISA and expression of COX-2 and iNOS by RT-PCR and western blotting in LPS stimulated RAW 264.7 macrophages. Total intracellular ROS was analyzed by DCFH-DA probing and the presence of quinazoline alkaloid (vasicine) in the ALK-F was evidenced by high performance liquid chromatography (HPLC). Results:The ALK-F of A. vasica exhibited a significant inhibitory effect on LPS elicited nitrite production (13.2 ± 1.06 µM), iNOS, and COX-2 (2.6 and 3.3 fold) in a dose-dependent manner. There was a significant decrease in the generation of these pro-inflammatory cytokines TNF-α (1102 ± 1.02 pg/mL) and IL-6 (18 ± 0.87 ng/mL) and total intracellular ROS in the highest tested concentrations (1 µg and 10 µg) of ALK-F of A. vasica. HPLC analysis by the gradient elution method revealed the presence of 12% of quinazoline alkaloid vasicine in the crude alkaloid fraction. Conclusion: Thus this study communally suggests that attenuation of nitric oxide and the dysregulation of genes responsible for inflammation which deliberates A. vasica to conflict against inflammation and provide remedial benefits in diabetic wound care.

show abstract

“…It is observed that Toll like receptor (TLR-2) expression is suppressed in collagen induced arthritis in mice with the AVE administration. TLR-2 (component of innate immune system) is expressed on the macrophages and dendritic cell surfaces that is responsible for B and T-lymphocyte response generation under pathogenic incursion [21] .…”

Section: Adhatoda Vasica Neesmentioning

confidence: 99%

Perspective of Plant Medicine in Therapy of Rheumatoid Arthritis

Gautam¹,

Roy²,

THAPA³

et al. 2020

ijps

View full text Add to dashboard Cite

Gautam et al.: Plant Medicine in Therapy of Rheumatoid ArthritisRheumatoid Arthritis is a systemic autoimmune disease characterized by chronic, inflammatory condition. The adverse effects of long-term use of presently available anti-arthritic or non-steroidal anti-inflammatory drugs are gastrointestinal symptoms, cardiovascular complications, renal impairment, myelosuppression etc. and this requires continuous monitoring and eventually increasing the cost of treatment. Thus complementary and alternative medicines may fulfill the demand for patients suffering from this disease. Moreover, herbal therapy has been safe and effective enough to treat rheumatoid arthritis. With these backgrounds, the present review includes different 37 plants reported for anti-arthritic or anti-inflammatory effect. Also enlisted 37 bioactive principals reported for anti-arthritic effect with their source, mechanism of action and commercial herbal products available in the market for treatment of rheumatoid arthritis. The compiled information regarding plants and their role in the treatment of rheumatoid arthritis will help to justify the use of plant-derived medicine in the therapy of rheumatoid arthritis in future.

show abstract

Protective effects of methanolic extract of Adhatoda vasica Nees leaf in collagen-induced arthritis by modulation of synovial toll-like receptor-2 expression and release of pro-inflammatory mediators

Cited by 14 publications

References 32 publications

Salvianolic Acid B Suppresses Inflammatory Mediator Levels by Downregulating NF-κB in a Rat Model of Rheumatoid Arthritis

Salvianolic Acid B Suppresses Inflammatory Mediator Levels by Downregulating NF-κB in a Rat Model of Rheumatoid Arthritis

Presence of pyrroloquinazoline alkaloid in Adhatoda vasica attenuates inflammatory response through the downregulation of pro-inflammatory mediators in LPS stimulated RAW 264.7 macrophages

Perspective of Plant Medicine in Therapy of Rheumatoid Arthritis

Contact Info

Product

Resources

About