Protective Effects of Mogroside V on Oxidative Stress Induced by H2O2 in Skin Fibroblasts

Abstract

Purpose Damage caused by oxidative stress leads to the premature aging of cells. Mogrosides, the main active components of Siraitia grosvenorii , have strong antioxidant activity; however, it is unclear whether mogroside V (MV) exerts these effects in skin cells. This was investigated in the present study by evaluating the protective effects of MV against oxidative damage induced by hydrogen peroxide (H 2 O 2 ) in skin fibroblasts. Methods Mouse skin fibroblasts (MSFs) were treated with H 2 O 2 and cell viability, total antioxidant capacity, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, and antioxidant enzyme activity were assessed. Results Treatment with MV reduced the ROS level and MDA content in MSFs treated with H 2 O 2 . This was accompanied by increased superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities. Conclusion MV reduces H 2 O 2 -induced oxidative stress and enhances endogenous antioxidant activity in skin fibroblasts. Thus, MV can potentially be used as an ingredient in anti-aging cosmetic products.

Potential Anti-Alzheimer Properties of Mogrosides in Vitamin B12-Deficient Caenorhabditis elegans

“…Our mogrosides extract was composed of mogrosides IIe, III, IV and V, where the mogroside V was the most abundant in the mixture ( Figure 1 ). Mogroside V is known to have protective effects against oxidative stress in skin fibroblast [ 37 ] and neuronal damages in mice [ 38 ], which supports our gene expression results. Gene expression studies using RT-qPCR indicated that mogrosides up-regulated the expression of oxidative-related genes, including sod-1 ( Figure 4 A), gpx-1 ( Figure 4 B) and clt-1 ( Figure 4 C) in N2 fed with OP50/B12 − , which supports previous research findings regarding the antioxidant properties of mogrosides [ 7 ].…”

Siraitia grosvenorii As a Homologue of Food and Medicine: A Review of Biological Activity, Mechanisms of Action, Synthetic Biology, and Applications in Future Food

“…38 Mogroside V specifically reduces ROS and MDA in H 2 O 2 -treated skin fibroblasts, increasing SOD, GSH-Px, and CAT activities. 39 Additionally, SGP polysaccharide (molecular weight 1.93 × 10 3 kDa) exhibits effective DPPH radical scavenging and reduces ROS and cell damage in H 2 O 2 -damaged PC12 cells in a dosedependent manner. 10 Aging and Ovarian Function: Aging, marked by a decline in bodily functions, 40 particularly affects the ovaries, which show significant functional decline after 35 years.…”

A Review of the Phytochemistry and Pharmacology of the Fruit of Siraitia grosvenorii (Swingle): A Traditional Chinese Medicinal Food

“…Meanwhile, mogroside V ( 16 ) promotes the anti-oxidative stress capacity of skin fibroblasts by modulating the scavenging of free radicals with antioxidant enzymes, which could be used to prevent skin aging or lesions. In MSF treated with H 2 O 2 , mogrosides V ( 16 ) reduced ROS levels and MDA content, together with an increase in superoxide dismutase (SOD), glutathione peroxide (GSH-Px), and catalase (CAT) activities [ 47 ]. Furthermore, mogroside extracts (MGE) inhibited BSA glycation, as evidenced by the formation of fluorescent advanced glycation end products (AGEs) at 500 μg/ml with lower levels of protein carbonate and Ne-(carboxymethyl) lysine.…”