Protective effects of Monotheca buxifolia fruit on renal toxicity induced by CCl4 in rats

Abstract

BackgroundOxidative stress is believed to be a root cause of various degenerative and fibrotic disorders. Dietary foods enrich in antioxidants can cure or curtail the progression of oxidative stress induced disorders. Fruit of Monotheca buxifolia is used locally for digestive and urinary tract disorders. We have evaluated the protective potential of the methanol extract of M. buxifolia (MBM) in rat exposed to carbon tetrachloride (CCl4) toxicity.MethodsPowder of the dried fruit of M. buxifolia was extracted twice with 95 % methanol to get the extract (MBM). Presence of polyphenolic constituents was detected by HPLC-DAD (High Performance Liquid Chromatography with Diode Array Detection) analysis. Sprague-Dawley male rats were divided in to six groups with six rats in each. Animals of Group I were kept control, while rats of Group II – Group V were treated intraperitoneally with 1 ml/kg body weight (bw) of CCl4 (30 % v/v; olive oil) 15 dosages in 30 days. Animals of Group III were orally administered silymarin (50 mg/kg bw) while Group IV and V with 200 and 400 mg/kg of MBM on next day of CCl4 treatment. Rats of Group VI were administered only with 400 mg/kg bw of MBM. Biochemical markers of the urine and serum were analyzed. Level of antioxidant enzymes, DNA damages lipid peroxides (TBARS), H2O2 and nitrite was assessed in renal tissues of rat. Histopathological changes in renal tissues of rat were also recorded.ResultsHPLC-DAD analysis of MBM indicated the existence of gallic acid, catechin, caffeic acid and rutin. MBM administration significantly alleviated the toxic effect of CCl4 in rat and decreased the elevated level of RBCs, pus and epithelial cells, specific gravity, creatinine, urobilinogen, urea and albumin while increased the pH and urinary protein. Increase in the level of urobilinogen, blood urea nitrogen (BUN), urea and total bilirubin while decrease of albumin and total protein in serum was restored by the administration of MBM to CCl4 fed rat. Administration of MBM to CCl4 exposed rats significantly increased the activity level of phase I and phase II enzymes and GSH while decreased the level of TBARS, H2O2, nitrite and DNA damages in renal tissues of rat. Furthermore, histopathological alterations induced with CCl4 in renal tissues of rat were also diminished with the administration of MBM.ConclusionRestoration of various parameters induced with toxic insult of CCl4 in rat suggests the antioxidant and repairing potential of M. buxifolia fruit in kidney disorders.

Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats

“…Our results revealed a significant increase in the LPO marker (i.e., MDA) and a significant decrease in SOD, CAT and GPx enzyme activities in rat tissues following CCl 4 application. This is in agreement with previous studies (Szymonik-Lesiuk et al, 2003; Alqasoumi & Abdel-Kader, 2012; Jan & Khan, 2016; Noureen et al, 2017). Treatment with rutin significantly decreased MDA and increased SOD, CAT and GPx activities.…”

“…The degree of toxicity depends on the overproduction of reactive oxygen species (ROS), oxidative injury and the inflammatory process. CCl 4 intoxication generates free radicals such as nitric oxide and peroxynitrite that trigger a cascade of events resulting in liver and kidney damage in rats (Khan & Zehra, 2013; Jan & Khan, 2016). Accordingly, we found significant elevation of serum ALP, AST, ALT, and bilirubin levels, while protein and albumin levels were significantly decreased in the CCl 4 -treated group.…”

Protective Effect of Capparis spinosa Extract against Potassium Bromate Induced Oxidative Stress and Genotoxicity in Mice

“…Furthermore, there were a considerable number of reports recently about the protective effects of C. spinosa such as antioxidant, hepatoprotective, and nephroprotective effects in various model organisms in vivo and in vitro [ 23 , 33 , 35 , 36 ]. This is because the plants provide an alternative source to scavenge free radicals due to the presence of diverse nature of secondary metabolites [ 37 ]. The observed protective effects of C. spinosa might be by virtue of the high levels of polyphenolic compounds and flavonoids in the extract [ 36 , 38 ].…”

Phytochemical, acute toxicity and renal protective appraisal of Ajuga parviflora hydromethanolic leaf extract against CCl4 induced renal injury in rats

“…Thirty-six rats were divided into six groups (six animals in each group). Group A animals were considered as naïve group (received no treatment) while animals in remaining groups received 1 mL/kg body weight CCl 4 (Sigma Aldrich, USA) intraperitoneally in 30% v/v olive oil for induction of renal toxicity [26]. However, Group B was considered as positive control (received CCl 4 only) while Group C was treated with Silymarin (Sigma Aldrich, USA) oral solution at a dose of 100 mg/kg as standard treatment.…”