Protective effects of Oviductus Ranae-containing serum on oxidative stress-induced apoptosis in rat ovarian granulosa cells

Ling, Xiaomei; Zhang, Xuhui; Tan, Yan; Yang, Jingjing; Jiang, Bo; Xin-rong, WU; Yi, Yankui; Liu, Liang

doi:10.1016/j.jep.2017.05.035

Cited by 34 publications

(21 citation statements)

References 38 publications

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“…Previous studies have reported that treatment with H 2 O 2 150 μM could induce damage in a variety of cell types [29,30]. Consistently, in this study, we also found that treatment with H 2 O 2 150 μM for 24 hr could inhibit the proliferation and promote the apoptosis in CPCs.…”

Section: Discussionsupporting

confidence: 91%

Pre-Treatment with Melatonin Enhances Therapeutic Efficacy of Cardiac Progenitor Cells for Myocardial Infarction

Ma¹,

He²,

Ding³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Melatonin possesses many biological activities such as antioxidant and anti-aging. Cardiac progenitor cells (CPCs) have emerged as a promising therapeutic strategy for myocardial infarction (MI). However, the low survival of transplanted CPCs in infarcted myocardium limits the successful use in treating MI. In the present study, we aimed to investigate if melatonin protects against oxidative stress-induced CPCs damage and enhances its therapeutic efficacy for MI. Methods: TUNEL assay and EdU assay were used to detect the effects of melatonin and miR-98 on H₂O₂-induced apoptosis and proliferation. MI model was used to evaluate the potential cardioprotective effects of melatonin and miR-98. Results: Melatonin attenuated H₂O₂-induced the proliferation reduction and apoptosis of c-kit⁺ CPCs in vitro, and CPCs which pretreated with melatonin significantly improved the functions of post-infarct hearts compared with CPCs alone in vivo. Melatonin was capable to inhibit the increase of miR-98 level by H₂O₂ in CPCs. The proliferation reduction and apoptosis of CPCs induced by H₂O₂ was aggravated by miR-98. In vivo, transplantation of CPCs with miR-98 silencing caused the more significant improvement of cardiac functions in MI than CPCs. MiR-98 targets at the signal transducer and activator of the transcription 3 (STAT3), and thus aggravated H₂O₂-induced the reduction of Bcl-2 protein. Conclusions: Pre-treatment with melatonin protects c-kit+ CPCs against oxidative stress-induced damage via downregulation of miR-98 and thereby increasing STAT3, representing a potentially new strategy to improve CPC-based therapy for MI.

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Section: Discussionsupporting

confidence: 91%

Pre-Treatment with Melatonin Enhances Therapeutic Efficacy of Cardiac Progenitor Cells for Myocardial Infarction

Ma¹,

He²,

Ding³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…However, pretreatment with these compounds, except for 11, increased the levels of Bcl‐2. Bcl‐2 is an antiapoptotic protein that triggers mitochondrial depolarization in the apoptosis pathway (Ling et al., ).…”

Section: Resultsmentioning

confidence: 99%

Comparison of Antioxidant Constituents of Agriophyllum squarrosum Seed with Conventional Crop Seeds

Zheng

Zhang

et al. 2018

Journal of Food Science

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“…In aging rats, compound Renshen-Hamayou capsule exhibited antioxidant effects through increasing GSH-Px activity and decreasing MDA level [88], while in mice of X-ray-induced oxidative stress, intragastric administration of compound Linwayou granule in doses of 100 and 200 mg/kg BW for 7 days can also remarkably elevate SOD and GSH-Px activities [89]. Ling et al explored the molecular mechanisms of the antioxidant activities of OR and observed that OR-containing serum elicits protective effects on oxidative stress-induced apoptosis in rat ovarian granulosa cells through decreasing apoptosis via reducing ROS production and improving mitochondrial membrane potential via downregulating p53, Bax, caspase-3, and caspase-9 and upregulating Bcl-2, as well as weakening the phosphorylation of JNK and p38 MAPK, and enhancing the phosphorylation of ERK1/2 [83]. At the gene level, oral administration of OR can upregulate the expression of antioxidant-related gene Cu/Zn-SOD both in young and in aging mice in different stages.…”

Section: Biological Functions Of Oviductus Ranaementioning

confidence: 99%

Traditional Uses, Bioactive Constituents, Biological Functions, and Safety Properties of Oviductus ranae as Functional Foods in China

Zhang

Wang

et al. 2019

Oxidative Medicine and Cellular Longevity

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Oviductus ranae is an animal-based traditional Chinese material widely used as tonics in China for hundreds of years. Various bioactive components are present in OR including proteins, amino acids, steroids, fatty acids, phospholipids, nucleosides, vitamins, hydantoins, and mineral elements. These constituents exert a myriad of biological functions such as immunomodulatory, antioxidant, antifatigue, antiaging, estrogen-like, hepatoprotective, hypolipidemic, antiosteoporotic, antidepressant, antitumor, antitussive, expectorant, anti-inflammatory, and antiasthmatic activities. Unlike other traditional Chinese crude drugs recorded in Chinese Pharmacopoeia, OR is seldom prescribed as medicine but often consumed as nutraceuticals to optimize health. In this review, the traditional uses, bioactive constituents, biological functions, and safety properties of OR as functional foods in China were summarized and discussed. It is expected that this review will provide useful information for anyone who is interested in OR.

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Protective effects of Oviductus Ranae-containing serum on oxidative stress-induced apoptosis in rat ovarian granulosa cells

Cited by 34 publications

References 38 publications

Pre-Treatment with Melatonin Enhances Therapeutic Efficacy of Cardiac Progenitor Cells for Myocardial Infarction

Pre-Treatment with Melatonin Enhances Therapeutic Efficacy of Cardiac Progenitor Cells for Myocardial Infarction

Comparison of Antioxidant Constituents of Agriophyllum squarrosum Seed with Conventional Crop Seeds

Traditional Uses, Bioactive Constituents, Biological Functions, and Safety Properties of Oviductus ranae as Functional Foods in China

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