Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice

Abstract

Objectives: The aim of the present study is to evaluate hepatoprotective effect of Picrorhizakurroaextracts (PKE) against Fumonisin b1 (Fb1) induced hepatotoxicity. Methods: Hepatoprotective analysis was done by estimating serum biochemical parameters Alanine aminotransferase (ALT) and aspartate aminotransferase (AST), antioxidant enzyme status lipid peroxidations, ROS generation, and protein carbonyls were also estimated to know the oxidative damage. Further protein expressions were confirmed by western blotting. Results: The mice treated with 100 and 200 mg/kg b.wt PKE, showed a significant reduction in serum AST and ALT to 175.41 ± 7.82, 68.52±1.57 and 126.64 ± 1.72, 57.41±1.28 units/ml of serum respectively. The decrease in antioxidant levels with Fb1 was significantly restored with 100 and 200 mg/kg by wt PKE. The lipid peroxidation products with Fb1 treatment was also reduced with PKE supplementation. The Fb1 induced ROS was reduced with 60 and 140% by 100 and 200 mg/kg/ by wt PKE administration. The PKE restored CAT and GPx enzyme/protein levels and further down regulated caspase 8 and caspase 3 expression. Conclusion: The present observations suggested that the treatment with PKE extract enhance the recovery from Fb1 induced hepatic damage due to its antioxidant and hepatoprotective property.

Protective effects of resveratrol against fumonisin B1-induced liver toxicity in mice

“…Our findings confirm reports of increased liver enzyme levels by the same FB 1 dose (2.25 mg/kg bw) ( 24 – 26 ). Administration of resveratrol in our study attenuated these effects to the levels no longer significantly higher than control.…”

The effects of resveratrol on SIRT2, SIRT3 expression levels and oxidative DNA damage in fumonisin-induced hepatotoxicity in BALB/c mice

“…The authors showed that ROS production was related to mitochondrial complex I, CYP450 and the NADPH oxidase system. KRUPASHREE et al (2018) reported that SOD, CAT, GSH-Px, and GSH levels decreased and MDA levels increased in hepatotoxicity induced by FB1 (2.25 mg) in BALB/c mice.…”

“…In this study, the effect of resveratrol on SIRT2, SIRT3 protein expressions, 8-OHdG, reduced GSH, nitric oxide and MDA levels were investigated in fumonisininduced hepatotoxicity in BALB/c mice. It has been reported that exposure to FB1 disrupts the redox-system and exerts this effect by increasing reactive oxygen species (ROS), lipid peroxidation, oxidative DNA damage and reducing antioxidant GSH content, GSH-Px and SOD levels (ABEL and GELDERBLOM, 1998;EL-NEKEETY et al, 2007;KRUPASHREE et al, 2018;MARY et al, 2012). The antioxidant approaches in in vivo and in vitro studies generally aim to reduce the toxic effects of FB1.…”

Nanoencapsulation of apocynin and vanillic acid extracted from Picrorhiza kurroa Royle ex Benth plant roots and its characterisation

“…picrosides I and II, Kutkins, kutkosides, cucurbitacin, sterols/triterpenes and simple phenols such as apocynin, vanillic acid most explored phytoactives from ethanolic and methanolic extracts [ 7 , 8 ]. The dried roots and rhizomes of the plant are commercially important parts, and they are used to treat a variety of ailments, including spleen disorders, liver diseases, and allergy issues [ 9 , 10 ]. The use of traditional medicinal plants in the form of functional foods and nutraceuticals has acquired significance in the modulation of numerous diseases, P. kurroa is one such therapeutic herb through which we could avoid the intake of chemical drugs to treat these disorders without any side effects.…”