Protective effects of polydatin on experimental testicular torsion and detorsion injury in rats

Abstract

Oxidative stress plays a critical role in the process of testicular torsion and detorsion (T/D). The purpose of the present study was to investigate the protective effect of polydatin (PD) on testicular T/D injury. Rats were randomly divided into three groups, a sham group, a group subjected to 2h torsion followed by 24h detorsion and a group subjected to T/D and injected i.p. with 20mgkg PD 30min before detorsion. Unilateral orchiectomy was performed after 24h of reperfusion. Half the testes were prepared for histological examination by haematoxylin-eosin staining and the terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) technique. In the remaining tissues, levels of malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) were determined, as was the expression of several apoptosis-related proteins. Compared with the T/D group, PD pretreatment significantly ameliorated the morphological damage, lowered the Cosentino histological score and increased the mean number of germ cell layers and Johnsen's testicular biopsy score. In addition, PD treatment markedly decreased MDA levels and upregulated CAT, GPx and SOD activity. Furthermore, PD decreased T/D-induced germ cell-specific apoptosis, attenuated the activation of caspase-3, caspase-8, caspase-9 and poly(ADP-ribose) polymerase and increased the Bcl-2/Bax ratio. The findings indicate that PD has a protective effect against testicular T/D injuries, especially at the histological, antioxidative stress and antiapoptotic levels.

FOXO1 targeting by capsaicin reduces tissue damage after testicular torsion

“…Ischaemia/reperfusion (IR) injury plays an important role in the pathophysiology of TT and affects the degree of atrophy and sperm count (Abdel‐Rahman, Mahmoud, Mostafa, Saleh, & Eissa, ; Mertoğlu et al., ). High content of polyunsaturated fatty acids in the plasma membrane of sperms increases their vulnerability to ROS and oxidative stress (Qiao et al., ; Rifaioglu et al., ; Yuluğ et al., ). Based on previous evidences, early detection and treatment can improve the results of the treatment and patients’ quality of life (Amani et al., ).…”

Protective Effects of Polydatin on Reproductive Injury Induced by Ionizing Radiation

“… 21 At the same time, polydatin can also reduce the damage to the testis of mice caused by ionizing radiation by inhibiting the ratio of apoptosis-related protein Bcl-2/Bax. 40 All the above studies have shown that polydatin has a significant radiation protection effect, but for reproductive damage induced by ionizing radiation, more experimental studies are still needed to demonstrate its effective dose and action mechanism.…”

Old dog, new tricks: Polydatin as a multitarget agent for current diseases

“…Testicular torsion desorption (T/D) is a common male disease, and the reduction of germ cells is inevitable. PD (20 mg/kg) significantly improved T/D‐related morphological injury, which increased the ratio of Bcl‐2/Bax to reduce apoptosis, reduced the level of MDA, and increased the expression of SOD to resist oxidation (Qiao et al, 2017). PD reduced arsenic‐induced lipid peroxidation and regenerated testicular tissue injury in rats (Ince et al, 2016).…”