Protective effects of polymethoxyflavone-rich cold-pressed orange peel oil against ultraviolet B-induced photoaging on mouse skin

Li, Guijie; Tan, Fang; Zhang, Qunlin; Tan, Anqun; Cheng, Yujiao; Zhou, Qi; Liu, Manjiao; Tan, Xiang; Huang, Linhua; Rouseff, Russell L.; Wu, Houjiu; Liang, Guolu; Zhao, Xiaochun

doi:10.1016/j.jff.2020.103834

Cited by 18 publications

(15 citation statements)

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“…Then, the PVDF membrane was sealed with 5% skim milk in Tris buffer containing Tween 20 (TBST) solution for 1 h. After sealing, the PVDF membrane was washed with TBST and incubated with primary antibodies against copper/zinc SOD (Cu/Zn-SOD), manganese SOD (Mn-SOD), catalase (CAT), GSH-Px, heme oxygenase-1 (HO-1), nuclear factor E2-related factor 2 (Nrf-2), COX-2, NO synthase (NOS) 1, NOS2, NOS3, and β-actin (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) at 4°C overnight. After that, the PVDF membrane was washed with TBST and then incubated with the corresponding secondary antibodies at 25°C for 1 h. According to the instructions of a SuperSignal West Pico Plus Kit, a chromogenic solution was prepared and used to completely cover the PVDF membrane; the membrane was then put into an iBright FL1000 imaging system (Thermo Fisher Scientific) for observation ( 18 ). In addition, ImageJ software was used to analyze the images, and β-actin was used as an internal reference protein to calculate the relative expression of the target proteins.…”

Section: Methodsmentioning

confidence: 99%

“…Then, 2 µL of cDNA template, 10 µL of SYBR Green PCR Master Mix (Thermo Fisher Scientific), 1 µL of upstream primer and 1 µL of downstream primer were mixed, and a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) was used. The PCR conditions were as follows: predenaturation at 95 • C for 3 min and 40 cycles of denaturation at 95 • C for 10 s, annealing at 57 • C for 30 s, and extension at 72 • C for 15 s. Using β-actin as a control, the relative transcription levels of mRNA were calculated by the 2 − CT method (18). Table 1 shows the primer sequence information for this study.…”

Section: Quantitative Real-time Pcr (Rt-qpcr) Assaymentioning

confidence: 99%

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Effects of Cold-Pressing and Hydrodistillation on the Active Non-volatile Components in Lemon Essential Oil and the Effects of the Resulting Oils on Aging-Related Oxidative Stress in Mice

Sha

Pan

et al. 2021

Front. Nutr.

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The aim of this study was to analyze the non-volatile composition and antioxidant differences of lemon essential oils (LEOs) obtained by cold-pressing vs. hydrodistillation. Pathological observations showed that LEO effectively inhibited liver injury caused by oxidative stress, and CPLEO was more effective than HDLEO. CPLEO increased serum T-AOC, SOD, GSH, and GSH-Px levels while decreasing NO, COX-2, IL-6, IL-1β, IFN-γ, and TNF-α levels in mice with oxidative damage. The effects of CPLEO were stronger than those of HDLEO and similar to those of vitamin C. CPLEO upregulated mRNA and protein expressions of Cu/Zn-SOD, Mn-SOD, CAT, HO-1, Nrf2, and NQO1 while downregulating nNOS, iNOS, IL-1β, COX-2, TNF-α, and NF-κB mRNA expression and nNOS, eNOS, iNOS, and COX-2 protein expression in mice with oxidative damage. The results demonstrate that LEO has good antioxidant effects and that CPLEO has a better antioxidant effect than HDLEO as it retains more active non-volatile substances.

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Section: Methodsmentioning

confidence: 99%

Section: Quantitative Real-time Pcr (Rt-qpcr) Assaymentioning

confidence: 99%

Effects of Cold-Pressing and Hydrodistillation on the Active Non-volatile Components in Lemon Essential Oil and the Effects of the Resulting Oils on Aging-Related Oxidative Stress in Mice

Sha

Pan

et al. 2021

Front. Nutr.

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“…Lemon has the effects of generating saliva, relieving heat and appetite, preventing cardiovascular diseases, clearing heat, and eliminating phlegm and has anti-bacterial, anti-inflammatory, and anti-aging effects (Zeng et al, 2018;Zhou et al, 2014;Zhu et al, 2018Zhu et al, , 2019. In addition to the pulp, lemon can be processed into juice and other food products, lemon leaves can be used for flavor extraction, fresh lemon fruit epidermis can be used to produce lemon essential oil, and lemon seeds can also be used for extraction edible oil for consumption (Li et al, 2020;Wang et al, 2019). However, only a small amount of lemon seed has been applied in the processing and application of lemon.…”

Section: Introductionmentioning

confidence: 99%

Effect of lemon seed flavonoids on the anti‐fatigue and antioxidant effects of exhausted running exercise mice

Zhang

Yang

2021

J. Food Biochem.

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In this research, mice were gavaged with different doses of lemon seed flavonoids (LSF) for 4 weeks, and vitamin C was used as a positive control to investigate its effects on anti-fatigue and antioxidant capacity in exhaustively exercised mice. The results obtained from the study indicated that both vitamin C and LSF could significantly increase the running exhaustion time of mice, and the exhaustion time of mice was prolonged with increasing LSF concentration. LSF can increase hepatic glycogen and the free fatty acid content and reduce the lactate and urea nitrogen contents in a dose-dependent manner in mice. Serum CK, AST, and ALT levels in mice decreased gradually with increasing LSF concentration. LSF increased SOD and CAT levels and decreased MDA levels in mice in a dose-dependent manner. LSF could also enhance nNOS, eNOS, and ASCT1 mRNA expression and decrease syncytin-1, iNOS and TNFα expression in the skeletal muscle of mice. By HPLC analysis, LSF was found to contain epigallocatechin, caffeic acid, epicatechin, vitexin, quercetin, and hesperidin, which are common flavonoids of this species. Thus, it was observed that LSF has good anti-fatigue and antioxidant capacities, and its anti-fatigue effect is related to improving the hepatic glycogen reserve capacity, increasing fat mobilization, and reducing lactate accumulation and protein decomposition. The antioxidant capacity of LSF is related to scavenging free radicals and reducing lipid peroxidation, and its antioxidant effect comes from its five antioxidant flavonoids. In conclusion, LSF has high development and application prospects in nutritional supplements. Practical applicationsLemon seed is the waste of lemon processing, which contains abundant flavonoids.The flavonoids in lemon seed can be used to exert its antioxidant effect and recover from exhausted exercise. Therefore, it can be concluded that lemon seed flavonoids are functional components that can be used as exercise recovery substances.

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“…The pulp and juice of lemon are commonly used, and lemon leaves can be used as a seasoning. The skin of fresh lemon fruit can be used to produce lemon essential oil, and edible oil can be obtained from squeezing lemon seeds [7,8]. However, lemon peels are not commonly used in the processing and application of lemons, and most of the lemon peels created during the industrial process of lemons are discarded as waste.…”

Section: Introductionmentioning

confidence: 99%

Effect of lemon peel flavonoids on anti-fatigue and anti-oxidation capacities of exhaustive exercise mice

2020

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In this study, lemon peel flavonoids (LPF) were administered to investigate its effect on the anti-fatigue and antioxidant capacity of mice that undergo exercise until exhaustion. LPF (88.36 min in LPFH group mice) significantly increased the exhaustion swimming time compare to the untreated mice (40.36 min), increased the liver glycogen and free fatty acid content in mice and reduce lactic acid and BUN content in a dose-dependent manner. As the concentration of lemon peel flavonoids increased, the serum creatine kinase, aspartate aminotransferase, and alanine aminotransferase levels of mice gradually decreased. LPF increases superoxide dismutase (SOD) and catalase (CAT) levels in mice and reduces malondialdehyde levels in a dose-dependent manner. And LPF raises hepatic tissue SOD, CAT activities and reduces skeletal muscle tissue iNOS, TNF-α levels of mice compared to the control group. LPF also enhanced the expression of copper/zinc-superoxide dismutase (Cu/Zn-SOD), manganese-superoxide dismutase (Mn-SOD), and CAT mRNA in mouse liver tissue. LPF also enhanced the expression of alanine/serine/cysteine/threonine transporter 1 (ASCT1) mRNA and attenuate the expression of syncytin-1, inducible nitric oxide synthase (iNOS), and tumor necrosis factor (TNF)-α in mouse skeletal muscle. According to high-performance liquid chromatography (HPLC) analysis, it was found that LPF contains flavonoids such as rutin, astragalin, isomangiferin, naringin, and quercetin. Our experimental data show that LPF has good anti-fatigue effects and anti-oxidation ability. In summary, LPF has high prospects to be developed and added to nutritional supplements.

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Protective effects of polymethoxyflavone-rich cold-pressed orange peel oil against ultraviolet B-induced photoaging on mouse skin

Cited by 18 publications

References 50 publications

Effects of Cold-Pressing and Hydrodistillation on the Active Non-volatile Components in Lemon Essential Oil and the Effects of the Resulting Oils on Aging-Related Oxidative Stress in Mice

Effects of Cold-Pressing and Hydrodistillation on the Active Non-volatile Components in Lemon Essential Oil and the Effects of the Resulting Oils on Aging-Related Oxidative Stress in Mice

Effect of lemon seed flavonoids on the anti‐fatigue and antioxidant effects of exhausted running exercise mice

Effect of lemon peel flavonoids on anti-fatigue and anti-oxidation capacities of exhaustive exercise mice

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