Protective effects of polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp on non-alcoholic fatty liver disease in high-fat diet rats via PPAR and AMPK signaling pathways

Zeng, Shunjiang; CHEN, Yuzi; Wei, Chenxi; Tan, Lei; Li, Chuan; Zhang, Yanjun; Xu, Fei; Zhu, Kexue; Wu, Gang; Cao, Jun

doi:10.1016/j.jff.2022.105195

Cited by 15 publications

(11 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SREBP1 is a transcription factor that stimulates hepatic fatty acid biosynthesis via upregulation of lipogenesisrelated genes, such as FAS (35). The uncontrolled activation of SREBP-1c is associated with hepatosteatosis, insulin resistance and pro-inflammatory signaling cascade (73). Circulating leptin levels are related to body fat mass.…”

Section: Discussionmentioning

confidence: 99%

Pediococcus pentosaceus PR-1 modulates high-fat-died-induced alterations in gut microbiota, inflammation, and lipid metabolism in zebrafish

et al. 2023

View full text Add to dashboard Cite

IntroductionObesity is a health issue worldwide. This study aimed to evaluate the beneficial effects of Pediococcus pentococcus PR-1 on the modulating of gut microbiota, inflammation and lipid metabolism in high-fat-diet (HFD)-fed zebrafish.MethodsAdult zebrafish were fed a commercial (C), high fat (H, 25% fat), probiotic (P, 106 CFU/g), or high fat with probiotic (HP) diets twice daily for 5 weeks. Gut microbiota were analysed using 16S rRNA gene sequencing. Gene expressions of intestinal cytokine, intestinal TJ protein, and liver lipid metabolism were analysed by quantitative real-time polymerase chain reaction. Biochemical and histological analysis were also performed.Results and discussionP. pentosaceus PR-1 reduced body weight and BMI, indicating its anti-obesity effect. The 16S rRNA sequencing results showed HFD induced a distinct gut microbiota structure from C group, which was restored by probiotic. P. pentosaceus PR-1 improved gut health by decreasing the abundance of Ralstonia and Aeromonas which were increased induced by HFD. Moreover, probiotic restored abundance of Fusobacteria, Cetobacterium and Plesiomonas, which were decreased in HFD-fed zebrafish. The results of quantitative real-time polymerase chain reaction showed probiotic suppressed HFD-induced inflammation by decreasing the expressions of IL-1b and IL-6. Levels of hepatic TNF-α, IL-1ß, and IL-6 were reduced by probiotic in HFD-fed zebrafish. Probiotic also ameliorated gut barrier function by increasing the expressions of occludin, Claudin-1, and ZO-1. Probiotic exerted anti-adipogenic activity through regulating the expressions of SREBP1, FAS and LEPTIN. Levels of hepatic triglyceride, total cholesterol, low density lipoprotein were also reduced by probiotic. Histological analysis showed probiotic alleviated liver steatosis and injury induced by HFD. P. pentosaceus PR-1 might be useful as a dietary health supplement, especially for reducing obesity.

show abstract

Section: Discussionmentioning

confidence: 99%

Pediococcus pentosaceus PR-1 modulates high-fat-died-induced alterations in gut microbiota, inflammation, and lipid metabolism in zebrafish

et al. 2023

View full text Add to dashboard Cite

show abstract

“…We can evaluate liver function by detecting the content of ALB. [26] Based on the targets and pathways analyses, the results showed that the potential mechanism of NBBP in the treatment of HLP might be most relevant to regulating the "NAFLD" pathway. This pathway mainly comprises fatty acid biosynthesis, PPAR, PI3K-Akt, TNF, and adipocytokine signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

“…The loss of substituent groups is the main characteristic of conjugated bile acids, such as À SO 3 H, À OH, À NH 2 . For example, compound 3 obtained quasi-molecular ion peak m/z 498.2895[M-H] À in negative ion mode, whose molecular formula is predicted to be C 26 Bile acids are the main component of NBBP and have an essential bioactive role in the protection against disorders of lipid metabolism. [18] Therefore, we chose bile acid as the active ingredient of NBBP for network pharmacology analysis.…”

Section: Identification Of Taurine-conjugated Bile Acidsmentioning

confidence: 99%

In Vivo and Network Pharmacological Studies of Natural Bear Bile Powder Against Hyperlipidemia

Wang,

Qin,

Tian

et al. 2023

ChemistrySelect

View full text Add to dashboard Cite

Natural bear bile powder (NBBP), a traditional Chinese medicine, has been clinically used to treat liver dysfunction, cardiovascular and cerebrovascular diseases. Recently it has been proved that NBBP has a good effect on regulating lipid disorders. However, the active ingredients and the anti‐hyperlipidemic effects are unclear. The high‐fat diet (HFD) induced hyperlipidemic model rats were treated with NBBP for 4 weeks and were examined for, lipid profile, liver function parameters, hepatic antioxidant status and histopathology of liver, epididymal adipose tissues. The NBBP treatment effectively improved serum lipid levels and normalized antioxidant status. A total of 16 constituents of NBBP were charactered via UHPLC‐Q‐TOF‐HRMS. We screened eight potentially active compounds and corresponded to 75 anti‐hyperlipidemic target proteins. PPI analysis showed that ALB, TNF, EGFE, PPARG, and PPARA are core proteins. Pathway enrichment analysis showed that NBBP might treat hyperlipidemia (HLP) by regulating a series of signaling pathways involving immune system, inflammatory response, and metabolism. Molecular docking revealed that gene had high affinities to NBBP compounds. In conclusion, UHPLC‐Q‐TOF‐HRMS analysis, network pharmacology, and in vivo experiments in this study provided essential references for revealing the potential mechanism of NBBP in the prevention and treatment of HLP.

show abstract

“…A polysaccharide from jackfruit pulp (JFP-Ps) was found to improve liver function in high-fat diets (HFD) -induced mice by modulating the expression of genes involved in lipid metabolism, such as peroxisome proliferator activated receptor alpha (PPARα), hormonesensitive lipase (HSL), carnitine palmitoyltransferase 1A (CPT1), lipoprotein lipase (LPL), acetyl-CoA carboxylase alpha (ACC), fatty acid synthase (FAS) and sterol regulatory element binding transcription factor (SREBP-1c) (12). The activation of PPAR and AMP-activated protein kinase (AMPK) signaling pathways may be responsible for its therapeutic effect.…”

Section: Therapeutic Effects Of Polysaccharides On Nafldmentioning

confidence: 99%

Research progress on the therapeutic effects of polysaccharides on non-alcoholic fatty liver diseases

Ma²,

Dong³

et al. 2023

Front. Nutr.

View full text Add to dashboard Cite

Non-alcoholic fatty liver disease (NAFLD) has become the most common chronic liver disease and is a leading cause of cirrhosis and hepatocellular carcinoma. Due to its complex pathophysiology, there is currently no approved therapy. Polysaccharide, a kind of natural product, possesses a wide range of pharmacological activities. Numerous preclinical studies have confirmed that polysaccharides could interfere with the occurrence and development of NAFLD at multiple interrelated levels, such as improvement of glucose and lipid metabolism, antioxidation, anti-inflammation, and regulation of gut-liver axis, thus showing great potential as novel anti-NAFLD drugs. In this paper, we reviewed the polysaccharides with anti-NAFLD effect in recent years, and also systematically analyzed their possible pharmacological mechanisms.

show abstract

Protective effects of polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp on non-alcoholic fatty liver disease in high-fat diet rats via PPAR and AMPK signaling pathways

Cited by 15 publications

References 45 publications

Pediococcus pentosaceus PR-1 modulates high-fat-died-induced alterations in gut microbiota, inflammation, and lipid metabolism in zebrafish

Pediococcus pentosaceus PR-1 modulates high-fat-died-induced alterations in gut microbiota, inflammation, and lipid metabolism in zebrafish

In Vivo and Network Pharmacological Studies of Natural Bear Bile Powder Against Hyperlipidemia

Research progress on the therapeutic effects of polysaccharides on non-alcoholic fatty liver diseases

Contact Info

Product

Resources

About