Protective effects of propolis on methotrexate-induced testis injury in rat

Sönmez, Mehmet Fatih; Çilenk, Kübra Tuğçe; Karabulut, Derya; Ünalmış, Sunay; Deligönül, Erkan; Öztürk, İsmet; Kaymak, Emin

doi:10.1016/j.biopha.2016.02.002

Cited by 46 publications

(41 citation statements)

References 34 publications

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“…[ 31 ] In another study investigating the protective effects of propolis on MTX‐induced testis injury in rats, the researchers demonstrated a significant decrease in HSP70 expression in the interstitial space and a significant increase in HSP70 expression in the germinal cells. [ 2 ] In our study, we found a significant increase in HSP70 expression in the interstitial cells in epididymides and an increase in HSP70 expression in the Leydig cells in testes. However, an increase in HSP70 in testes was not significant.…”

Section: Discussionsupporting

confidence: 57%

“…Therefore, it is much more toxic than other pharmaceutical agents. [ 2 ] MTX is an effective anti‐inflammatory, antiproliferative, immunosuppressive, and antipsychotic folic acid antagonist. [ 3,4 ] Recently, MTX has been used to treat many diseases including rheumatoid arthritis, psoriasis, systemic lupus erythematosus, dermatomyositis, lymphoma, leukemia, osteosarcoma, and lung and breast cancer.…”

Section: Introductionmentioning

confidence: 99%

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Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats

Aslankoç

Özmen

Elli̇dağ

2020

J Biochem & Molecular Tox

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The aim of this study was to investigate the possible prophylactic effects of agomelatine (AGO) against testicular and epididymal damage induced by methotrexate (MTX) in rats. Twenty‐four male Wistar albino rats were divided into three groups: Group I (control group), Group II (MTX group: 20 mg/kg MTX, i.p, single dose), and Group III (MTX+AGO group: 20 mg/kg MTX, i.p, single dose+40 mg/kg AGO; gavage, 7 days). The rats were killed under anesthesia 24 hours after the last AGO application. Testicular and epididymal tissues were bilaterally removed for morphometric, biochemical, pathological, and immunohistochemical analyses. Body, testicular, and epididymal weights were measured. Malondialdehyde (MDA), superoxide dismutase, catalase, and glutathione peroxidase levels were measured in testes. Sperm count, hyperemia, edema, inflammatory reaction, degenerated and necrotic cells were evaluated by histopathological analysis. In addition, inducible nitric oxide synthase (iNOS), granulocyte colony‐stimulating factor (G‐CSF), osteopontin (OPN), and heat shock protein‐70 (HSP70) immune reactions were analyzed in testes and epididymides. Decreased epididymal weights, increased MDA levels, decreased sperm count, hyperemia, edema, inflammatory reaction, and degenerated and necrotic cells were observed in the MTX group. In addition, iNOS, HSP70, G‐CSF, and OPN immune reactions were increased. AGO improved morphometric, biochemical, histopathological, and immunohistochemical findings. The present study confirms that MTX induces testicular and epididymal damage both biochemically and immunohistochemically. However, AGO demonstrated ameliorative effects on both biochemical and pathological findings of the current study.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats

Aslankoç

Özmen

Elli̇dağ

2020

J Biochem & Molecular Tox

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“…The release of HSPs increases many types of cellular stress such as anoxia, viral infections, toxic compounds, oxidative stress and the absence of glucose (Sönmez et al, ). HSPs protect structures of destabilised or denatured proteins during stress.…”

Section: Discussionmentioning

confidence: 99%

The effects of high‐fructose corn syrup consumption on testis physiopathology—The ameliorative role of melatonin

Aslankoç

Özmen

2019

Andrologia

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This study investigated the ameliorative role of melatonin (MLT) and the effects of a long‐term intake of high‐fructose corn syrup (HFCS) on the male reproductive system. Thirty‐six male Sprague Dawley rats were randomly divided into 3 groups as follows: Control, HFCS and HFCS + MLT. Testis and epididymal weights were measured. Malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities, total testosterone levels, testicular histopathological damage scores were evaluated, and immunohistochemical analyses were performed on testicular tissue. Epididymal weights were significantly lower in the HFCS + MLT group than those of the control and HFCS groups. MDA was significantly increased, while SOD and CAT activities were reduced in the HFCS group compared with the control group. Administration of melatonin significantly increased SOD and CAT activities in the HFCS + MLT group. Histopathological evaluation revealed slight hyperaemia and oedema in the stromal tissue of rat testes in the HFCS group. Sperm count and Johnsen's testicular biopsy score (JTBS) were significantly decreased in the HFCS group. Immunohistochemical analysis revealed that HSP, iNOS, MDA, OPN and VEGF values were significantly increased in the HFCS group. However, melatonin ameliorated the immunohistochemical scoring. Our results showed that a long‐term intake of HFCS caused testicular damage. Melatonin may be a promising pharmacological agent against testicular toxicity induced by HFCS.

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“…The chilling is one of the detrimental factors that induce the production of ROS in extended semen of rabbits [5,6]. Propolis was used in this study as a natural additive to semen extender owing to its high contents of polyphenolic compounds, vitamins, minerals and other antioxidants in addition to its antinflammatory, antibacterial, immunoregulatory and strong cytoprotective effect against some toxic stimuli [48][49][50][51][52].…”

Section: Discussionmentioning

confidence: 99%

Preservability of rabbit semen after chilled storage in tris based extender enriched with different concentrations of Propolis ethanolic extract (PEE)

El-Seadawy

El-Nattat

El-Tohamy

et al. 2017

APJR

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Protective effects of propolis on methotrexate-induced testis injury in rat

Cited by 46 publications

References 34 publications

Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats

Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats

The effects of high‐fructose corn syrup consumption on testis physiopathology—The ameliorative role of melatonin

Preservability of rabbit semen after chilled storage in tris based extender enriched with different concentrations of Propolis ethanolic extract (PEE)

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