Protective effects of protopine on hydrogen peroxide-induced oxidative injury of PC12 cells via Ca2+ antagonism and antioxidant mechanisms

Xiao, Xianghua; Liu, Juntian; Hu, Jingwen; Zhu, Xiuping; Yang, Hua; Wang, Chaoyun; Zhang, Yuanhui

doi:10.1016/j.ejphar.2008.06.045

Cited by 106 publications

(56 citation statements)

References 25 publications

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“…Energy failure in oxygen and glucose deprivationinduced ischemia can evoke the accumulation of intracellular Ca 2+ by enhancing the entry of Ca 2+ through NMDA receptors or release of Ca 2+ from the mitochondria through the Na + -Ca 2+ exchanger, and interfering with the ATP-dependent extrusion and sequestration of Ca 2+ [24] . Stained elevated Ca 2+ levels induced by OGD-Rep may impair mitochondrial function preceded by the opening of a permeability transition pore in the inner mitochondrial membrane, excessive release of glutamate, activate phospholipase, protease and endonucleases, eventually leading to neuronal apoptosis and death [25,26] . Apoptosis is driven from the activation of a family of cysteine protease called caspases, which modulate the mitochondria-and death receptor-mediated pathways, respectively.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of ginsenoside Rb3 on oxygen and glucose deprivation-induced ischemic injury in PC12 cells

et al. 2010

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Aim: To investigate the protective effects of ginsenoside Rb 3 , a triterpenoid saponin isolated from the leaves of Panax notoginseng, on ischemic and reperfusion injury model of PC12 cells and elucidate the related mechanisms. Methods: PC12 cells exposed to oxygen and glucose deprivation (OGD) and restoration (OGD-Rep) were used as an in vitro model of ischemia and reperfusion. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) leakage were used to evaluate the protective effects of ginsenoside Rb 3 . Cellular apoptosis and mitochondrial membrane potential (MMP) were analyzed using flow cytometry. Intracellular calcium ion concentration ([Ca 2+ ] i ) was detected using fluorophotometer system. Caspase-3, -8, and -9 activities were measured using assay kits with an ELISA reader. Western blotting assay was used to evaluate the release of cytochrome c and expression of caspase-3, Bcl-2 and Bax proteins. Results: It was shown that ginsenoside Rb 3 (0.1-10 μmol/L) significantly increased cell viability and inhibited LDH release in a dosedependent manner on the ischemic model. In addition, ginsenoside Rb 3 also significantly inhibited ischemic injury-induced apoptosis, [Ca 2+ ] i elevation, and decrease of MMP. Meanwhile, pretreatment with ginsenoside Rb 3 significantly induced an increase of Bcl-2 protein expression and a decrease of cytosolic cytochrome c, cleaved-caspase 3 and Bax protein expression, the caspase-3, -8, and -9 activity were also inhibited. Conclusion:The results indicated that ginsenoside Rb 3 could markedly protected OGD-Rep induced ischemic injury and the mechanisms maybe related to its suppression of the intracellular Ca 2+ elevation and inhibition of apoptosis and caspase activity. Ginsenoside Rb 3 could be a promising candidate in the development of a novel class of anti-ischemic agent.

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Section: Discussionmentioning

confidence: 99%

Protective effects of ginsenoside Rb3 on oxygen and glucose deprivation-induced ischemic injury in PC12 cells

et al. 2010

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“…Protopine Antioxidant Enhanced the activity of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase and catalase [104] . Hepatopretective Inhibited microsomal lipid peroxidation [105] .…”

Section: Anxiolyticmentioning

confidence: 99%

Chelidonium majus L. (Greater celandine) – A Review on its Phytochemical and Therapeutic Perspectives

Maji¹,

Banerji²

2015

Int. J. Herb. Med.

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Chelidonium majus L. (Papaveraceae) is a medicinal herb used in various traditional systems of medicine to treat ulcer, cancer, oral infection, liver disorders, chronic bronchitis, asthma, etc. Different parts of this plant contain numerous therapeutically important alkaloidal constituents such as chelidonine, chelerythrine, sanguinarine, berberine and so on. The plant and its active compounds exhibit a wide range of pharmacological activities. The plants has long history of therapeutic use in medicines without any toxic effect. Today it is one of the important components of some pharmaceutical preparations. Consumption of C. majus preparations possesses toxic effects on the liver although the effect becomes controversial with its hepatoprotective effect. Numerous active constituents of C. majus interact with various drug metabolizing enzymes that mimic possible interactions of this herb with the conventional drugs. This review provides detailed phytochemical, pharmacological and toxicological information of C. majus along with mechanisms of action of its various active compounds on different aspects of pharmacology. This review also highlights plausible drug interaction of its various active compounds and the future prospect of this herb.

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“…PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. Differentiated PC12 cells induced by nerve growth factor (NGF) have the typical characteristics of the neurons in form and function, which therefore are widely used as a model in vitro for the neuron research, such as the apoptosis and the differentiation of neuron (Ishima et al, 2008;Xiao et al, 2008). The viability of the cells was observed by a 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays.…”

Section: Introductionmentioning

confidence: 99%

Effect of melamine on potassium currents in rat hippocampal CA1 neurons

Yang

Tian

Yang

et al. 2010

Toxicology in Vitro

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Protective effects of protopine on hydrogen peroxide-induced oxidative injury of PC12 cells via Ca2+ antagonism and antioxidant mechanisms

Cited by 106 publications

References 25 publications

Protective effects of ginsenoside Rb3 on oxygen and glucose deprivation-induced ischemic injury in PC12 cells

Protective effects of ginsenoside Rb3 on oxygen and glucose deprivation-induced ischemic injury in PC12 cells

Chelidonium majus L. (Greater celandine) – A Review on its Phytochemical and Therapeutic Perspectives

Effect of melamine on potassium currents in rat hippocampal CA1 neurons

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