Protective effects of quercetin on nicotine induced oxidative stress in ‘HepG2 cells’

Yarahmadi, Amir; Zal, Fatemeh; Bolouki, Ayeh

doi:10.1080/15376516.2017.1344338

Cited by 49 publications

(38 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, we investigated the direct effects of three doses (10 − 11 , 10 − 8 and 10 − 6 M) of nicotine on global genomic DNA methylation and DNMTs gene expression in HESC. The rationale for using 10 − 11 , 10 − 8 and 10 − 6 M concentrations were that we have reported previously that the nicotine doses below 10 − 6 M are safe for using in the in-vitro model of human cells [30,35]. Our results indicated that treatments of HESC with 10 − 11 and 10 − 8 M of nicotine significantly decreased the level of DNMT1 gene expression by 98 and 86.5% in comparison to control cells ( Fig.…”

Section: Discussionsupporting

confidence: 54%

Nicotine attenuates global genomic DNA methylation by influencing DNMTs gene expression in human endometrial stromal cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background: There is increasing evidence indicating an incidence of infertility and also the risk of endometrial cancers among smokers. However, the mechanism underlying nicotine adverse effect on female reproduction remains unclear. Growing evidence has suggested that environmental exposures such as nicotine could modulate the epigenome. No study has yet been published to evaluate the direct effect of nicotine on the epigenome profiling of human endometrial stromal cells (HESC). Herein, we decided to examine the direct effects of nicotine on global genomic DNA methylation status and DNA methyl-transferases (DNMTs) gene expression in HESC. HESC were treated with different doses of nicotine (0 or control, 10 − 11 , 10 − 8 and 10 − 6) M for 24 h and their genomic global DNA methylation and gene expression of DNMTs (DNMT1, DNMT3A, and DNMT3B) were investigated using ELISA and real-time PCR, respectively. Results: Nicotine treatments reduced the average level of DNMTs gene expression by 90, 79, and 73.4% in 10 − 11 , 10 − 8 and 10 − 6 M of nicotine treated cells as compared to control cells, respectively (p < 0.05). Also, 10 − 8 and 10 − 6 M of nicotine concentrations effectively reduced the amounts of 5-methylated cytosine (5-mC) by 1.09 and 1.87% compared to control cells, respectively (p < 0.05). The 5-mC percentages were positively correlated with the relative cellular DNMTs expression in HESC as verified by the Pearson correlation test. Conclusion: An interesting possibility raised by the current study is that the reduced genomic global DNA methylation level in HESC may be partly due to the suppression of DNMTs gene expression caused by nicotine in these cells.

show abstract

Section: Discussionsupporting

confidence: 54%

Nicotine attenuates global genomic DNA methylation by influencing DNMTs gene expression in human endometrial stromal cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Quercetin protects the nerves, brain, or other body cells from oxidation-induced damage by regulating the antioxidant levels [31]. Quercetin prevents free radicals and enhances the body's antioxidant defense systems and therefore reduces oxidative stress, including the production of nicotine-induced ROS for the treatment of diseases such as nicotine addiction [38]. In vivo studies have shown that quercetin has antioxidant and hepatoprotective activity against acute hepatic injury caused by tertiary butyl hydrogen peroxide.…”

Section: Antioxidant Activitymentioning

confidence: 99%

The Pharmacological Activity, Biochemical Properties, and Pharmacokinetics of the Major Natural Polyphenolic Flavonoid: Quercetin

Batiha

Beshbishy

Ikram

et al. 2020

Foods

472

245

View full text Add to dashboard Cite

Flavonoids are a class of natural substances present in plants, fruits, vegetables, wine, bulbs, bark, stems, roots, and tea. Several attempts are being made to isolate such natural products, which are popular for their health benefits. Flavonoids are now seen as an essential component in a number of cosmetic, pharmaceutical, and medicinal formulations. Quercetin is the major polyphenolic flavonoid found in food products, including berries, apples, cauliflower, tea, cabbage, nuts, and onions that have traditionally been treated as anticancer and antiviral, and used for the treatment of allergic, metabolic, and inflammatory disorders, eye and cardiovascular diseases, and arthritis. Pharmacologically, quercetin has been examined against various microorganisms and parasites, including pathogenic bacteria, viruses, and Plasmodium, Babesia, and Theileria parasites. Additionally, it has shown beneficial effects against Alzheimer’s disease (AD), and this activity is due to its inhibitory effect against acetylcholinesterase. It has also been documented to possess antioxidant, antifungal, anti-carcinogenic, hepatoprotective, and cytotoxic activity. Quercetin has been documented to accumulate in the lungs, liver, kidneys, and small intestines, with lower levels seen in the brain, heart, and spleen, and it is extracted through the renal, fecal, and respiratory systems. The current review examines the pharmacokinetics, as well as the toxic and biological activities of quercetin.

show abstract

“…Moreover, EGCG has been shown to chelate transition metals that increase ROS levels by the Fenton reaction and to indirectly upregulate the expression of phase II antioxidant enzymes [50]. Numerous other compounds present in the TE and LE extracts, such as the caffeic acid derivatives, quercetin, and its esters, have also been shown to have a protective effect against nicotine induced cytotoxicity [9,10,[51][52][53]. .…”

Section: Cytocompatibility and Cytoprotective Effect Of Le And Te Agamentioning

confidence: 99%

Phytochemical Profile and Biological Activities of Tendrils and Leaves Extracts from a Variety of Vitis vinifera L.

et al. 2020

View full text Add to dashboard Cite

Winery industry by-products have a great reuse potential in the pharmaceutical and cosmetic fields due to their bioactive compounds. This study investigates the phytochemical profile and the bioactivity of Vitis vinifera variety Fetească neagră tendrils extract (TE) and leaves extract (LE), intended to be used in oral hygiene products recommended in periodontal disease. The evaluation of the phenolic content was performed by colorimetric analysis. Liquid chromatography coupled with mass spectrometry was used to determine the chemical profile of the two extracts obtained from V. vinifera. Moreover, the antioxidant activity of the extracts was determined by spectrophotometric methods, as well as on human gingival fibroblasts (HGF) cell line. The cytocompatibility and cytoprotective effect against nicotine-induced cytotoxicity were tested, as well as the anti-inflammatory and antimicrobial effects. The TE showed higher total polyphenolic content, rich in rutin, compared to the leaves extract that displayed important amounts of isoquercitrin. The antioxidant effect was confirmed by both non-cellular and cellular tests. The cytocompatibility of the extracts was confirmed at a wide range of concentrations. The cytoprotective effect was demonstrated in HGF exposed to cytotoxic doses of nicotine; 300 µg/mL of both tested extracts decreased nicotine toxicity by approximately 20%. When challenged with E. coli polysaccharides, in HGF cells co-exposed to TE and LE, a reduction in the release of proinflammatory cytokines (IL-8, IL-6 and IL-1β) was observed. The extracts were both able to reduce the levels of reactive oxygen species and inflammatory cytokines, and had notable antimicrobial effects on pathogenic bacteria associated with periodontitis.

show abstract

Protective effects of quercetin on nicotine induced oxidative stress in ‘HepG2 cells’

Cited by 49 publications

References 49 publications

Nicotine attenuates global genomic DNA methylation by influencing DNMTs gene expression in human endometrial stromal cells

Nicotine attenuates global genomic DNA methylation by influencing DNMTs gene expression in human endometrial stromal cells

The Pharmacological Activity, Biochemical Properties, and Pharmacokinetics of the Major Natural Polyphenolic Flavonoid: Quercetin

Phytochemical Profile and Biological Activities of Tendrils and Leaves Extracts from a Variety of Vitis vinifera L.

Contact Info

Product

Resources

About