Protective effects of recombinant lactoferrin with different iron saturations on enteritis injury in young mice

Fan, L L; Yao, Qianqian; Wu, Haoming; Wen, F; Wang, J Q; Li, H Y; Zheng, N

doi:10.3168/jds.2021-21428

Cited by 19 publications

(15 citation statements)

References 40 publications

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“…On the other hand, iron absorption was reduced during enteritis ( Naveh et al., 2000 ). However, the supplement lactoferrin has prevented intestinal inflammation ( Fan et al., 2022 ), emphasizing a role for Deferribacteres. In comparison with the N group, Bacteroidetes and Actinobacteria proportions were lower in the intestinal microbiota in the mice of the TB group, which was consistent with the results of a previous study ( Yan et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium

Kong

Shang²,

Liu³

et al. 2023

Front. Cell. Infect. Microbiol.

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Terminalia bellirica (Gaertn.) Roxb. (TB) is a traditional herbal combination used in Chinese medicine for the treatment of a broad range of diseases. In this study, thirty KM mice were randomly divided into control (N), infection group (NS), and the TB protection group (HS). Based on its digestive feature, intestinal physical barrier, immunological barrier and gut microbiota effects in vivo on challenged with S.typhimurium mice were investigated after oral administration of 600 mg/kg b.wt of TB for 13 days. The results show that the extract could improve the level of serum immunoglobulins (IgA and IgG), decrease the intestinal cytokine secretion to relieve intestinal cytokine storm, reinforce the intestinal biochemical barrier function by elevating the sIgA expression, and strengthen the intestinal physical barrier function. Simultaneously, based on the V3–V4 region of the 16S rRNA analyzed, the results of the taxonomic structure of the intestinal microbiota demonstrated that the TB prevention effect transformed the key phylotypes of the gut microbiota in S. Typhimurium-challenged mice and promoted the multiplication of beneficial bacteria. Furthermore, the abundance of Firmicutes and Deferribacteres increased, while that of Bacteroidetes and Actinobacteria decreased. At the genus level, the abundance of Ruminococcus and Oscillospira was substantially enhanced, while the other dominant genera showed no significant change between the vehicle control groups and the TB prevention groups. In summary, these results provide evidence that the administration of TB extract can prevent S. Typhimurium infection by alleviating the intestinal physical and immunological barriers and normalizing the gut microbiota, highlighting a promising application in clinical treatment. Thus, our results provide new insights into the biological functions of TB for the preventive effect of intestinal inflammation.

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Section: Discussionmentioning

confidence: 99%

Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium

Kong

Shang²,

Liu³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Oral administration of liposomal bovine lactoferrin (LbLf) effectively prevents the progression of rheumatoid arthritis (RA) in mice by inhibiting tumor necrosis factor (TNF-α) production in the pancreas ( 37 ). In addition, Fan et al found that apo-LF, compared to holo-LF, could significantly attenuate LPS-induced inflammatory bowel injury by modulating the PPAR-γ/PFKFB3/NF-κB inflammatory pathway ( 38 ).…”

Section: Physicochemical Properties and Applications Of Lactoferrinmentioning

confidence: 99%

Lactoferrin: A glycoprotein that plays an active role in human health

Cao

Ren

et al. 2023

Front. Nutr.

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Lactoferrin (Lf), existing widely in human and mammalian milk, is a multifunctional glycoprotein with many functions, such as immune regulation, anti-inflammation, antibacterial, antiviral, and antioxidant. These extensive functions largely attribute to its ability to chelate iron and interfere with the cellular receptors of pathogenic microorganisms and their hosts. Moreover, it is non-toxic and has good compatibility with other supplements. Thus, Lf has been widely used in food nutrition, drug carriers, biotechnology, and feed development. Although Lf has been continuously explored and studied, a more comprehensive and systematic compendium is still required. This review presents the recent advances in the structure and physicochemical properties of Lf as well as clinical studies on human diseases, with the aim of providing a reference for further research of Lf and the development of its related functional products.

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“…Four treatment groups were set up, control group, apo-LF alone treatment group, LPS alone treatment group, and apo-LF and LPS combined treatment group. According to a previous study, we chose the concentrations of apo-LF and LPS [ 20 ]. The upper chamber was supplemented with FBS-free DMEM/F12 medium containing 10 g/L apo-LF, 1 mg/L LPS and 10 g/L apo-LF + 1 mg/L LPS, respectively.…”

Section: Methodsmentioning

confidence: 99%

The Protective Effects of Iron Free Lactoferrin on Lipopolysaccharide-Induced Intestinal Inflammatory Injury via Modulating the NF-κB/PPAR Signaling Pathway

Fan

Gao

et al. 2022

Foods

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Research evidence shows that effective nutritional intervention could prevent or reduce intestinal inflammatory injury in newborn infants. Iron free lactoferrin (apo-LF), one of the main types of lactoferrin (LF), is a bioactive protein in milk that plays a vital role in maintaining intestinal health. The potential mechanism by which apo-LF modulates intestinal inflammation is, however, still unclear. In the study we first explored key genes and pathways in vitro by transcriptome date analysis and then validated them in vivo to reveal the underlying molecular mechanism. The results showed that apo-LF pretreatment effectively inhibited lipopolysaccharide (LPS)-induced primary intestinal epithelial cells (IECs) inflammation in the co-culture system (primary IECs and immune cells), which was specifically manifested as the reduction of the concentration of TNF-α, IL-6 and IL-1β and increased the concentration of IFN-γ. In addition, transcriptome data analysis revealed that the key pathway for apo-LF to exert anti-inflammatory effects was the NF-κB/PPAR signaling pathway. Further validation was performed using western blotting in colonic tissues of young mice and it was found that the major proteins of NF-κB signaling pathway (NF-κB, TNF-α and IL-1β) were inhibited by apo-LF and the target proteins of PPAR signaling pathway (PPAR-γ and PFKFB3) were activated by apo-LF. Taken together, this suggests that apo-LF has a protective effect against LPS-induced intestinal inflammatory injury via modulating the NF-κB/PPAR signaling pathway, which provides new insights for further anti-inflammatory study of apo-LF.

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Protective effects of recombinant lactoferrin with different iron saturations on enteritis injury in young mice

Cited by 19 publications

References 40 publications

Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium

Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium

Lactoferrin: A glycoprotein that plays an active role in human health

The Protective Effects of Iron Free Lactoferrin on Lipopolysaccharide-Induced Intestinal Inflammatory Injury via Modulating the NF-κB/PPAR Signaling Pathway

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