Protective effects of resveratrol and its analogs on age-related macular degeneration in vitro

Abstract

Damage of retinal pigment epithelial (RPE) cells by A2E may be critical for age-related macular degeneration (AMD) management. Accumulation and photooxidation of A2E are known to be one of the critical causes in AMD. Here, we evaluated the protective effect of resveratrol (RES), piceatannol (PIC) and RES glycones on blue-light-induced RPE cell death caused by A2E photooxidation. A2E treatment followed by blue light exposure caused significant damages on human RPE cells (ARPE-19). But the damages were attenuated by post- and pre-treatment of RES and PIC in our in vitro models. The results of cell free system and FAB-MS analysis clearly showed that the reduction of A2E by blue light exposure was significantly rescued, and that oxidized forms of A2E were significantly reduced by RES or PIC treatment. Besides, RES or PIC inhibited the intracellular accumulation of A2E. Not only RES and PIC but RES glycones showed protection of ARPE-19 cells against A2E and blue-light-induced photo-damage. These findings demonstrate that RES and its analogs may have protective effects against A2E and blue-light-induced ARPE-19 cell death through regulation of A2E accumulation as well as photooxidation of A2E. Thus RES and its analogs may be beneficial for AMD treatment.

Sirtuins and Their Role in the Aging Eye (Review)

“…и соавт. обнаружили, что ресвератрол и его аналоги защищают клетки сетчатки и вызванного HEV-излучением фото-повреждения [38]. В исследовании Richer и соавт.…”

Protective effect of resveratrol against light-induced retinal degeneration in aged SAMP8 mice

“…Those studies provided increasing evidence that RESV has beneficial effects in neurodegenerative diseases such as AD [ 26 ], Parkinson’s Disease (PD) [ 27 ], and Amyotropic Lateral Sclerosis (ALS) [ 28 , 29 ]. Recently, protective effects of RESV have been investigated in vitro on retinal pigment epithelium cell death resulting from blue-light-induced damage [ 30 ], and in vivo on the rat model of diabetic retinopathy [ 31 ]. In this study, the effect of RESV in light-induced retinal damage of SAMP8 mice was analyzed to understand the mechanism of restoration of function of the affected retina following RESV administration in an aging animal model.…”

PFN1 and integrin‐β1/mTOR axis involvement in cornea differentiation of fibroblast limbal stem cells

“…In vitro and in vivo experiments have provided evidence about the biological effects of some natural plant polyphenols, therefore proposed as complement treatment in ocular diseases. In particular, resveratrol (RSV), a grape stilbenoid, revealed a modest beneficial impact on numerous cellular pathways implicated in age‐related ocular disorders, such as oxidative stress, inflammation, mitochondrial dysfunction, apoptosis and survival . Recent studies have demonstrated that RSV acts in a dose‐ and time‐dependent manner on MSCs, including human primary keratinocytes .…”

“…In particular, resveratrol (RSV), a grape stilbenoid, revealed a modest beneficial impact on numerous cellular pathways implicated in age-related ocular disorders, such as oxidative stress, inflammation, mitochondrial dysfunction, apoptosis and survival. [24][25][26] Recent studies have demonstrated that RSV acts in a dose-and time-dependent manner on MSCs, including human primary keratinocytes. [27][28][29] In the last few years, our group has focused on the pluripotent and immune-modulator characteristics in f-LSCs defined by a proteomic profile especially enriched with remodelling and wound healing proteins, including pfn1 and integrin-1β.…”